Theoretical and Applied Genetics

, Volume 118, Issue 6, pp 1173–1180 | Cite as

Inheritance and mapping of powdery mildew resistance gene Pm43 introgressed from Thinopyrum intermedium into wheat

  • Runli He
  • Zhijian Chang
  • Zujun Yang
  • Zongying Yuan
  • Haixian Zhan
  • Xiaojun Zhang
  • Jianxia Liu
Original Paper


Powdery mildew resistance from Thinopyrum intermedium was introgressed into common wheat (Triticum aestivum L.). Genetic analysis of the F1, F2, F3 and BC1 populations from powdery mildew resistant line CH5025 revealed that resistance was controlled by a single dominant allele. The gene responsible for powdery mildew resistance was mapped by the linkage analysis of a segregating F2 population. The resistance gene was linked to five co-dominant genomic SSR markers (Xcfd233, Xwmc41, Xbarc11, Xgwm539 and Xwmc175) and their most likely order was Xcfd233Xwmc41Pm43Xbarc11Xgwm539Xwmc175 at 2.6, 2.3, 4.2, 3.5 and 7.0 cM, respectively. Using the Chinese Spring nullisomic-tetrasomic and ditelosomic lines, the polymorphic markers and the resistance gene were assigned to chromosome 2DL. As no powdery mildew resistance gene was previously assigned to chromosome 2DL, this new resistance gene was designated Pm43. Pm43, together with the identified closely linked markers, could be useful in marker-assisted selection for pyramiding powdery mildew resistance genes.


Powdery Mildew Fusarium Head Blight Powdery Mildew Resistance Barley Yellow Dwarf Virus Powdery Mildew Resistance Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors are grateful to Drs. Bernd Friebe, Chengdao Li and Robert McIntosh for critical reviews of this manuscript, and to Dr. Shubing Liu for technical guidance in the SSR analyses. This project was funded by National Natural Science Foundation (30671299 and 39870398) and Shanxi Key Technologies R and D Program of China.


  1. Autrique E, Singh RP, Tanksley SD, Sorrells ME (1995) Molecular markers for four leaf rust resistance genes introgressed into wheat from wild relatives. Genome 38:75–83PubMedGoogle Scholar
  2. Ayala L, Henry M, Gonzalez-de-Leon D, van Ginkel M, Mujeeb-Kazi A, Keller B, Khairallah M (2001) A diagnostic molecular marker allowing the study of Th. intermedium-derived resistance to BYDV in bread wheat segregating populations. Theor Appl Genet 102:942–949CrossRefGoogle Scholar
  3. Blanco A, Gadaleta A, Cenci A, Carluccio AV, Abdelbacki AMM, Simeone R (2008) Molecular mapping of the novel powdery mildew resistance gene Pm36 introgressed from Triticum turgidum var. dicoccoides in durum wheat. Theor Appl Genet 117:135–142PubMedCrossRefGoogle Scholar
  4. Bougot Y, Lemoine J, Pavoine MT, Barloy D, Doussinault G (2002) Identification of a microsatellite associated with Pm3 resistance alleles to powdery mildew in wheat. Plant Breed 121:325–329CrossRefGoogle Scholar
  5. Cauderon Y, Saigne B, Dauge M (1973) The resistance to wheat rusts of Agropyron intermedium and its use in wheat improvement. In: Sears ER, Sears LMS (eds) Proc 4th Int Wheat Genet Symp. University of Missouri Agricutural Research Station, Columbia, pp 401–407Google Scholar
  6. Chang ZJ (1999) Production and molecular cytogenetic characterization of several Thinopyrum intermedium-derived wheat germplasm lines. PhD Dissertation, Sichuan Agricultural University, ChinaGoogle Scholar
  7. Chang ZJ, Yuan ZY, Guo XR, Yang ZJ, Ren ZL (2001) Production and genome analysis of a new 56-chromosome line derived from wheat × Agropyron intermedium. Proc Int Wheat Genetics and Breeding Symp, May 9–11, 2001, Zhengzhou, Henan. China Agricultural Scientech Press, Beijing, 237–241Google Scholar
  8. Chen Q (2005) Detection of alien chromatin introgression from Thinopyrum into wheat using S genomic DNA as a probe-a landmark approach for Thinopyrum genome research. Cytogenet Genome Res 109:350–359PubMedCrossRefGoogle Scholar
  9. Chen Q, Conner RL, Laroche A, Thomas JB (1998) Genome analysis of Thinopyrum intermedium and Th. ponticum using genomic in situ hybridization. Genome 41:580–586PubMedCrossRefGoogle Scholar
  10. Chen Q, Conner RL, Laroche A, Ahmad F (2001) Molecular cytogenetic evidence for a high level of chromosome pairing among different genomes in Triticum aestivum-Thinopyrum intermedium hybrids. Theor Appl Genet 102:847–852CrossRefGoogle Scholar
  11. Chen Q, Conner RL, Li HJ, Sun SC, Ahmad F, Laroche A, Graf RF (2003) Molecular cytogenetic discrimination and reaction to wheat streak mosaic virus and the wheat curl mite in the Zhong series of wheat-Thinopyrum intermedium partial amphiploids. Genome 46:135–145PubMedCrossRefGoogle Scholar
  12. Chen XM, Luo YH, Xia XC, Xia LQ, Chen X, Ren ZL, He ZH, Jia JZ (2005) Chromosomal location of powdery mildew resistance gene Pm16 in wheat using SSR marker analysis. Plant Breed 124:225–228CrossRefGoogle Scholar
  13. Dong YS, Bu XL, Luan YS, He MY, Liu B (2004) Molecular characterization of a cryptic wheat–Thinopyrum intermedium translocation line: evidence for genomic instability in nascent allopolyploid and aneuploid lines. Genet Mole Bio 27:237–241Google Scholar
  14. Fedak G (1999) Molecular aids for integration of alien chromatin through wide crosses. Genome 42:584–591CrossRefGoogle Scholar
  15. Fedak G, Han F (2005) Characterization of derivatives from wheat-Thinopyrum wide crosses. Cytogenet Genome Res 109:350–359CrossRefGoogle Scholar
  16. Friebe B, Jiang J, Raupp WJ, McIntosh RA, Gill BS (1996) Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status. Euphytica 91:59–87CrossRefGoogle Scholar
  17. Gupta PK, Varshney RK, Sharma PC, Ramesh B (1999) Molecular markers and their applications in wheat breeding. Plant Breed 118:369–390CrossRefGoogle Scholar
  18. Gupta PK, Balyan HS, Edwards KJ, Isaac P, Korzun V, Röder M, Gautier MF, Joudrier P, Schlatter AR, Dubcovsky J, De la Pena RC, Khairallah M, Penner G, Hayden MJ, Sharp P, Keller B, Wang RCC, Hardouin JP, Jack P, Leroy P (2002) Genetic mapping of 66 new microsatellite (SSR) loci in bread wheat. Theor Appl Genet 105:413–422PubMedCrossRefGoogle Scholar
  19. Han FP, Fedak G, Benabdelmouna A, Armstrong KC, Ouellet T (2003) Characterization of six wheat × Thinopyrum intermedium derivatives by GISH, RFLP and multicolor GISH. Genome 46:490–495PubMedCrossRefGoogle Scholar
  20. Huang XQ, Röder MS (2004) Molecular mapping of powdery mildew resistance genes in wheat: a review. Euphytica 137:203–223CrossRefGoogle Scholar
  21. Huang XQ, Hsam SLK, Zeller FJ, Wenzel G, Mohler V (2000) Molecular mapping of wheat powdery mildew resistance gene Pm24 and marker validation for molecular breeding. Theor Appl Genet 101:407–414CrossRefGoogle Scholar
  22. Huang XQ, Wang LX, Xu MX, Röder MS (2003) Microsatellite mapping of the powdery mildew resistance gene Pm5e in common wheat (Triticum aestivum L.). Theor Appl Genet 106:858–865PubMedGoogle Scholar
  23. Huang XQ, Hsam SLK, Mohler V, Röder MS, Zeller F (2004) Genetic mapping of three alleles at the Pm3 locus conferring powdery mildew resistance in common wheat (Triticum aestivum L.). Genome 47:1130–1136PubMedCrossRefGoogle Scholar
  24. Järve K, Peusha HO, Tsybalova J, Tamm S, Devos KM, Enno TM (2000) Chromosomal location of a Triticum timopheevi derived powdery mildew resistance gene transferred to common wheat. Genome 43:377–381PubMedCrossRefGoogle Scholar
  25. Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175Google Scholar
  26. Kuraparthy V, Sood S, Chhuneja P, Dhaliwal HS, Kaur S, Bowder RL, Gill BS (2007) A cryptic wheat–Aegilops triuncialis translocation with leaf rust resistance gene Lr58. Crop Sci 47:1995–2003CrossRefGoogle Scholar
  27. Larkin PJ, Banks PM, Lagudah ES, Appels R, Chen X, Xin XY, Ohm HW, McIntosh RA (1995) Disomic Thinopyrum intermedium addition lines in wheat with barley yellow dwarf virus resistance and with rust resistance. Genome 38:385–394PubMedCrossRefGoogle Scholar
  28. Li ZS, Rong S, Chen SY, Zhong GC, Mu SM (1985) Wheat wide hybridization. Chinese Scientific Press, Beijing, pp 52–58Google Scholar
  29. Lillemo M, Asalf B, Singh RP, Huerta-Espino J, Chen XM, He ZH, Bjørnstad Å (2008) The adult plant rust resistance loci Lr34/Yr18 and Lr46/Yr29 are important determinants of partial resistance to powdery mildew in bread wheat line Saar. Theor Appl Genet 116:1155–1166CrossRefPubMedGoogle Scholar
  30. Lincoln SE, Daly MJ, Lander ES (1993) Constructing linkage maps with MAPMAKER/Exp Version 3.0. A tutorial reference manual, 3rd edn. Whitehead Institute for Medical Res, CambridgeGoogle Scholar
  31. Liu SB, Wang HG (2005) Characterization of a wheat–Thinopyron intermedium substitution line with resistance to powdery mildew. Euphytica 143:229–233CrossRefGoogle Scholar
  32. Liu J, Liu D, Tao W, Li W, Wang S, Chen P, Cheng S, Gao D (2000) Molecular marker-facilitated pyramiding of different genes for powdery mildew resistance in wheat. Plant Breed 119:21–24CrossRefGoogle Scholar
  33. Liu ZY, Sun QX, Ni ZF, Nevo E, Yang TM (2002) Molecular characterization of a novel powdery mildew resistance gene Pm30 in wheat originating from wild emmer. Euphytica 123:21–29CrossRefGoogle Scholar
  34. Liu SB, Wang HG, Zhang XY, Li XF, Li DY, Duan XY, Zhou YL (2005) Molecular Cytogenetic identification of a wheat-Thinopyron intermedium (Host) Barkworth and DR Dewey partial amphiploid resistant to powdery mildew. J Integr Plant Biol 47:726–733CrossRefGoogle Scholar
  35. Ma ZQ, Wei JB, Chen SH (2004) PCR based markers for the powdery mildew resistance gene Pm4a in wheat. Theor Appl Genet 109:140–145PubMedCrossRefGoogle Scholar
  36. McIntosh RA, Yamazaki Y, Dubcovsky J, Rogers J, Morris C, Somers DJ, Appels R, Devos KM (2008) Catalogue of Gene Symbols for Wheat. Proc 11th Int Wheat Genet Symp, University of Sydney Press, Australia.
  37. Michelmore RM, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832PubMedCrossRefGoogle Scholar
  38. Miranda LM, Murphy JP, Leath S, Marshall DS (2006) Pm34: a new powdery mildew resistance gene transferred from Aegilops tauschii Coss. to common wheat (Triticum aestivum L.). Theor Appl Genet 113:1497–1504PubMedCrossRefGoogle Scholar
  39. Miranda LM, Murphy JP, Marshall DS, Cowger C, Leath S (2007) Chromosomal location of Pm35, a novel Aegilops tauschii derived powdery mildew resistance gene introgressed into common wheat (Triticum aestivum L.). Theor Appl Genet 114:1451–1456PubMedCrossRefGoogle Scholar
  40. Paillard S, Schnurbusch T, Winzeler M, Messmer M, Sourdille P, Abderhalden O, Keller B, Schachermayr G (2003) An integrative genetic linkage map of wheat. Theor Appl Genet 107:1235–1242PubMedCrossRefGoogle Scholar
  41. Perugini LD, Murphy JP, Marshall D, Brown-Guedira G (2008) Pm37, a new broadly effective powdery mildew resistance gene from Triticum timopheevii. Theor Appl Genet 116:417–425PubMedCrossRefGoogle Scholar
  42. Pestova E, Ganal MW, Röder MS (2000) Isolation and mapping of microsatellite markers specific for the D genome of bread wheat. Genome 43:689–697CrossRefGoogle Scholar
  43. Plaschke JB, Börner A, Wendehake K, Ganal MW, Röder MS (1996) The use of aneuploids for the chromosomal assignment of microsatellite loci. Euphytica 89:33–40CrossRefGoogle Scholar
  44. Qi L, Friebe B, Zhang P, Gill BS (2007) Homoeologous recombination, chromosome engineering and crop improvement. Chromosome Res 15:3–19PubMedCrossRefGoogle Scholar
  45. Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023PubMedGoogle Scholar
  46. Sharp PJ, Kreis M, Shewry PR, Gale MD (1988) Location of β-amylase sequence in wheat and its relatives. Theor Appl Genet 75:289–290CrossRefGoogle Scholar
  47. Sheng BQ, Duan XY, Zhang XX (1986) The improved adult resistance scales of wheat powdery mildew. Plant Prot Sin 3:44–45Google Scholar
  48. Shi QM, Zhang XX, Duan XY (1987) Identification of isolates of Blumeria graminis f. sp. tritici. Sci Agric Sin 20:64–70Google Scholar
  49. Singrün CH, Hsam SL, Zeller FJ, Mohler V (2003) Powdery mildew resistance gene Pm22 is a member of the complex Pm1 locus in common wheat (Triticum aestivum L). Theor Appl Genet 106:1420–1424PubMedGoogle Scholar
  50. Somers DJ, Isaac P, Edwards K (2004) A high density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114PubMedCrossRefGoogle Scholar
  51. Spielmeyer W, Singh RP, McFadden H, Wellings CR, Huerta-Espino J, Kong X, Appels R, Lagudah ES (2008) Fine scale genetic and physical mapping using interstitial deletion mutants of Lr34/Yr18: a disease resistance locus effective against multiple pathogens in wheat. Theor Appl Genet 116:481–490PubMedCrossRefGoogle Scholar
  52. Stephenson P, Bryan G, Kirby J, Collins A, Devos K, Busso C, Gale M (1998) Fifty new microsatellite loci for the wheat genetic map. Theor Appl Genet 100:564–568Google Scholar
  53. Sun SC (1981) The approach and methods of breeding new varieties and new species from Agrotriticum hybrids. Acta Agron Sin 7:51–58Google Scholar
  54. Wang RR-C, van Bothmer R, Dvórak R, Fedak G, Linde-Laursen I, Muramatsu M (1994) Genome symbols in the Triticeae (Poaceae). In: Wang RR-C, Jensen KB, Jaussi C (eds) Proc 2nd Int Triticeae Symp. Utah State University Press, Logan, pp 29–34Google Scholar
  55. Wang ZL, Li LH, He ZH, Duan XY, Zhou YL, Chen XM, Lillemo M, Singh RP, Wan H, Xia XC (2005) Seedling and adult plant resistance to powdery mildew in Chinese bread wheat cultivars and lines. Plant Dis 89:457–463CrossRefGoogle Scholar
  56. Xiang QJ, Sheng BQ, Zhou YL, Duan XY, Zhang KC (1994) Analyses of resistance genes of three differential varieties to the isolates of Blumeria graminis f. sp. tritici in wheat. Acta Agric Boreali-Sin 9:94–97Google Scholar
  57. Xie CJ, Sun QX, Ni ZF, Yang ZM, Nevo E, Fahima T (2003) Chromosomal location of a Triticum dicoccoides-derived powdery mildew resistance gene in common wheat by using microsatellite markers. Theor Appl Genet 106:341PubMedGoogle Scholar
  58. Yahiaoui N, Srichumpa P, Dudler R, Keller B (2004) Genome analysis at different ploidy levels allows cloning of the powdery mildew resistance Pm3b from hexaploid wheat. Plant J 34:528–538CrossRefGoogle Scholar
  59. Zhang XY, Koul A, Petroski R, Ouellet T, Fedak G, Dong YS, Wang RR-C (1996) Molecular verification and characterization of BYDV-resistant germplasms derived from hybrids of wheat with Thinopyrum ponticum and Th. intermedium. Theor Appl Genet 93:1033–1039CrossRefGoogle Scholar
  60. Zhou R, Zhu Z, Kong X, Huo N, Tian Q, Li C, Jin P, Dong Y, Jia J (2005) Development of wheat near-isogenic lines for powdery mildew resistance. Theor Appl Genet 110:640–648PubMedCrossRefGoogle Scholar
  61. Zhu ZD, Zhou RH, Kong XY, Dong YC, Jia JZ (2005) Microsatellite markers linked to two genes conferring resistance to powdery mildew in common wheat introgressed from Triticum carthlicum accession PS5. Genome 48:585–590PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Runli He
    • 1
  • Zhijian Chang
    • 1
    • 2
  • Zujun Yang
    • 3
  • Zongying Yuan
    • 4
  • Haixian Zhan
    • 2
  • Xiaojun Zhang
    • 2
  • Jianxia Liu
    • 1
  1. 1.School of Life Science and TechnologyShanxi UniversityTaiyuanChina
  2. 2.Institute of Crop GeneticsShanxi Academy of Agricultural SciencesTaiyuanChina
  3. 3.School of Life Science and TechnologyUniversity of Electronic Science and Technology of ChinaChengduChina
  4. 4.Institute of Plant ProtectionShanxi Academy of Agricultural SciencesTaiyuanChina

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