Theoretical and Applied Genetics

, Volume 118, Issue 6, pp 1121–1131 | Cite as

Development and genetic mapping of microsatellite markers from genome survey sequences in Brassica napus

  • Xiaomao Cheng
  • Jinsong Xu
  • Shu Xia
  • Jianxun Gu
  • Yuan Yang
  • Jie Fu
  • Xiaoju Qian
  • Shunchang Zhang
  • Jiangsheng Wu
  • Kede Liu
Original Paper

Abstract

Microsatellite or simple sequence repeat (SSR) markers are routinely used for tagging genes and assessing genetic diversity. In spite of their importance, there are limited numbers of SSR markers available for Brassica crops. A total of 627 new SSR markers (designated BnGMS) were developed based on publicly available genome survey sequences and used to survey polymorphisms among six B. napus cultivars that serve as parents for established populations. Among these SSR markers, 591 (94.3%) successfully amplified at least one fragment and 434 (73.4%) detected polymorphism among the six B. napus cultivars. No correlation was observed between SSR motifs, repeat number or repeat length with polymorphism levels. A linkage map was constructed using 163 newly developed BnGMS marker loci and anchored with 164 public SSRs in a doubled haploid population. These new markers are evenly distributed over all linkage groups (LGs). Given that the majority of these SSRs are derived from bacterial artificial chromosome (BAC) end sequences, they will be useful in the assignment of their cognate BACs to LGs and facilitate the integration of physical maps with genetic maps for genome sequencing in B. napus.

Notes

Acknowledgments

This research was supported by the National Key Basic Research and Development Plan of China (2006CB101600) and the National Science Foundation of China (30623012). We thank M. Mazourek for critical review of this manuscript.

Supplementary material

122_2009_967_MOESM1_ESM.xls (362 kb)
Supplementary material 1 (XLS 362 kb)

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Xiaomao Cheng
    • 1
  • Jinsong Xu
    • 1
  • Shu Xia
    • 1
  • Jianxun Gu
    • 1
  • Yuan Yang
    • 1
  • Jie Fu
    • 1
  • Xiaoju Qian
    • 1
  • Shunchang Zhang
    • 1
  • Jiangsheng Wu
    • 1
  • Kede Liu
    • 1
  1. 1.National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene ResearchHuazhong Agricultural UniversityWuhanChina

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