Genetic analysis of nuclear-cytoplasmic incompatibility in pea associated with cytoplasm of an accession of wild subspecies Pisum sativum subsp. elatius (Bieb.) Schmahl.
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The genetic basis of nuclear-cytoplasmic incompatibility was examined using the wild pea (Pisum sativum subsp. elatius) accession VIR320. When this accession is used as the female parent in crosses with domesticated peas (Pisum sativum subsp. sativum) the F1 is highly sterile and displays chlorophyll deficiency, chlorophyll variegation, reduction of leaflets and stipulae while the reciprocal cross produces hybrids that appear normal. A mapping recombinant inbred line (RIL) population was established based on a cross in a compatible direction of a tester line WL1238 with VIR320. The ability to cause nuclear-cytoplasmic conflict was analysed by crossing individual RIL plants as pollen parents with VIR320 as donor of cytoplasm and scoring each F1 for major signs of the conflict. It is concluded that two unlinked nuclear genes are involved in the genetic control of the observed incompatibility. One of the genes, denoted as Scs1, is closely linked to the PhlC gene on linkage group III and the other, denoted as Scs2, is closely linked to the gp gene on linkage group V. Alleles of both genes in WL1238 are dominant and appear to be lethal in the homozygous condition in the VIR320 cytoplasm background.
KeywordsRecombinant Inbred Line Recombinant Inbred Line Population Cleave Amplify Polymorphic Sequence Alloplasmic Line Wild Parent
The authors are grateful to Dr. N·F.Weeden for helpful comments on the manuscript and revising English language, to the anonymous referees for valuable suggestions and to Mrs. L.P. Romkina for excellent technical assistance. Sequencing reactions were performed at DNA Sequencing Center (ICG-ICBFM SD RAS). This work was supported by Russian Foundation for Fundamental Research, grant number 07-04-00111-a.
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