Characterization of low-molecular-weight glutenin subunit Glu-B3 genes and development of STS markers in common wheat (Triticum aestivum L.)
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Low-molecular-weight glutenin subunit (LMW-GS) Glu-B3 has a significant influence on the processing quality of the end-use products of common wheat. To characterize the LMW-GS genes at the Glu-B3 locus, gene-specific PCR primers were designed to amplify eight near-isogenic lines and Cheyenne with different Glu-B3 alleles (a, b, c, d, e, f, g, h and i) defined by protein electrophoretic mobility. The complete coding regions of four Glu-B3 genes with complete coding sequence were obtained and designated as GluB3-1, GluB3-2, GluB3-3 and GluB3-4. Ten allele-specific PCR markers designed from the SNPs present in the sequenced variants discriminated the Glu-B3 proteins of electrophoretic mobility alleles a, b, c, d, e, f, g, h and i. These markers were validated on 161 wheat varieties and advanced lines with different Glu-B3 alleles, thus confirming that the markers can be used in marker-assisted breeding for wheat grain processing quality.
KeywordsCommon Wheat Glutenin Subunit Complete Code Sequence Advanced Line Protein Allele
The authors are very grateful to Prof. Robert McIntosh, University of Sydney, for reviewing this manuscript. Aroona and its NILs were kindly provided by Dr. Marie Appelbee and Prof. Ken Shepherd, SARDI Grain Quality Research Laboratory, Adelaide, South Australia, Cheyenne was provided by Dr. Baoyun Li, China Agricultural University, Beijing, and Chinese Spring and nulli-tetrasomic lines N1A-T1D, N1B-T1A, N1B-T1D and N1D-T1B were provided by Prof. Robert McIntosh. The study was supported by the National Science Foundation of China (30671296 and 30830072), National Basic Research Program (2009CB118300) and National 863 Programs (2006AA10Z1A7 and 2006AA100102).
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