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Identification of differentially expressed proteins between hybrid and parents in wheat (Triticum aestivum L.) seedling leaves

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Abstract

In spite of commercial use of heterosis in agriculture, the molecular basis of heterosis is poorly understood. To gain a better understanding of the molecular basis of wheat heterosis, we carried out a comparative proteomic analysis in seedling leaves between wheat hybrid and parents. Common wheat (Triticum aestivum L., 2n = 6x = 42, AABBDD) Line 3338 and spelt wheat (Triticum spelta L., 2n = 6x = 42, AABBDD) Line 2463 were used to produce a heterotic F1 hybrid. The expression patterns of the total proteins were compared in seedling leaves between hybrid and its parents by using two-dimensional gel electrophoresis with two pH ranges for the first dimension separation. Among ~900 protein spots reproducibly detected, 49 protein spots were identified as being differentially expressed between hybrid and its parental lines (P < 0.05) for more than 1.5-folds. Six possible modes of differential expression were observed, including high- and low-parent dominance, underdominance, and overdominance, uniparent silencing and uniparent dominance. Moreover, 30 of the 49 differentially expressed protein spots were identified, which were involved in metabolism, signal transduction, energy, cell growth and division, disease and defense, secondary metabolism. These results indicated that wheat hybridization can cause protein expression differences between hybrid and its parents; these proteins were involved in diverse physiological process pathways, which might be responsible for the observed heterosis.

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Abbreviations

ACN:

Acetonitrile

ADP:

Adenosine diphosphate

ATP:

Adenosine triphosphate

BPH:

Best-parent heterosis

CHA:

Chemical hybridizing agent

CHAPS:

3-[(3-Cholamidopropyl)-dimethylammonio]-1-propane sulfonate

CHCA:

A-cyano-4-hydroxycinnamic acid

DDRT:

Differential display reverse transcript

DRH:

Down-regulated in hybrid

DTT:

1,4-Dithio-DL-threitol

FBA:

Fructose-1,6-bisphosphate aldolase

GO:

Gene ontology

HDH:

High-dominant in hybrid

IEF:

Isoelectric focusing

LDH:

Low-dominant in hybrid

LDW:

Leaf dry weight

LFW:

Leaf fresh weight

LRR:

Leucine-rich repeat protein

MPH:

Mid-parent heterosis

MS:

Mass spectrometry

PMF:

Peptide map fingerprinting

PTM:

Post translational modification

RcbA:

Rubisco activase

RcbL:

Rubisco large subunit

SSH:

Suppression subtractive hybridization

2DE:

Two-dimensional gel electrophoresis

TFA:

Trifluoroacetic acid

TIR:

Toll/interleukin-1 receptor

TLN:

Total leaf number

TTN:

Total tiller number

UPF1:

Dominant expression of uniparental proteins in hybrids

UPnF1:

Dominant expression of uniparental proteins but not in hybrids

URH:

Up-regulated in hybrid

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Acknowledgments

The authors wish to thank Mr. Jidong Feng for his assistance in mass spectrometry analysis. This work was financially supported by National Basic Research Program of China (2007CB109000), National Natural Science Foundation of China (30671297), Program for New Century Excellent Talents in University (No. NCET-05-0131) and 863 Project of China.

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Authors and Affiliations

  1. Key Laboratory of Crop Heterosis and Utilization (MOE), China Agricultural University, Yuanmingyuan Xi Road No. 2, Haidian district, 100193, Beijing, China

    Xiao Song, Zhongfu Ni, Yingyin Yao, Yinhong Zhang & Qixin Sun

  2. State Key Laboratory for Agrobiotechnology, China Agricultural University, 100193, Beijing, China

    Xiao Song, Zhongfu Ni, Yingyin Yao, Yinhong Zhang & Qixin Sun

  3. Key Laboratory of Crop Genomics and Genetic Improvement (MOA), China Agricultural University, 100193, Beijing, China

    Xiao Song, Zhongfu Ni, Yingyin Yao, Yinhong Zhang & Qixin Sun

  4. Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, 100193, Beijing, China

    Xiao Song, Zhongfu Ni, Yingyin Yao, Yinhong Zhang & Qixin Sun

  5. National Plant Gene Research Centre (Beijing), 100193, Beijing, China

    Xiao Song, Zhongfu Ni, Yingyin Yao, Yinhong Zhang & Qixin Sun

Authors
  1. Xiao Song
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  2. Zhongfu Ni
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  3. Yingyin Yao
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  4. Yinhong Zhang
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Corresponding authors

Correspondence to Zhongfu Ni or Qixin Sun.

Additional information

Communicated by M. Kearsey.

X. Song and Z. Ni have equally contributed to this work.

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122_2008_890_MOESM1_ESM.doc

Supplementary Table 1 t-test for the protein spot accumulation differences between wheat hybrid and parents (DOC 592 kb)

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Song, X., Ni, Z., Yao, Y. et al. Identification of differentially expressed proteins between hybrid and parents in wheat (Triticum aestivum L.) seedling leaves. Theor Appl Genet 118, 213–225 (2009). https://doi.org/10.1007/s00122-008-0890-4

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  • DOI: https://doi.org/10.1007/s00122-008-0890-4

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