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Theoretical and Applied Genetics

, Volume 118, Issue 1, pp 165–175 | Cite as

Identification of QTLs for resistance to powdery mildew and SSR markers diagnostic for powdery mildew resistance genes in melon (Cucumis melo L.)

  • Nobuko FukinoEmail author
  • Takayoshi Ohara
  • Antonio J. Monforte
  • Mitsuhiro Sugiyama
  • Yoshiteru Sakata
  • Miyuki Kunihisa
  • Satoru Matsumoto
Original Paper

Abstract

Powdery mildew caused by Podosphaera xanthii is an important foliar disease in melon. To find molecular markers for marker-assisted selection, we constructed a genetic linkage map of melon based on a population of 93 recombinant inbred lines derived from crosses between highly resistant AR 5 and susceptible ‘Earl’s Favourite (Harukei 3)’. The map spans 877 cM and consists of 167 markers, comprising 157 simple sequence repeats (SSRs), 7 sequence characterized amplified region/cleavage amplified polymorphic sequence markers and 3 phenotypic markers segregating into 20 linkage groups. Among them, 37 SSRs and 6 other markers were common to previous maps. Quantitative trait locus (QTL) analysis identified two loci for resistance to powdery mildew. The effects of these QTLs varied depending on strain and plant stage. The percentage of phenotypic variance explained for resistance to the pxA strain was similar between QTLs (R 2 = 22–28%). For resistance to pxB strain, the QTL on linkage group (LG) XII was responsible for much more of the variance (41–46%) than that on LG IIA (12–13%). The QTL on LG IIA was located between two SSR markers. Using an independent population, we demonstrated the effectiveness of these markers. This is the first report of universal and effective markers linked to a gene for powdery mildew resistance in melon.

Keywords

Quantitative Trait Locus Linkage Group Powdery Mildew Melon Composite Interval Mapping 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by Green Technology Project (grant DM-1607) from the Ministry of Agriculture, Forestry and Fisheries of Japan and by KAKENHI 19580043 from the Ministry of Education, Culture, Sports, Science and Technology. We are grateful to Dr. M. Kuzuya, Plant Biotechnology Institute, Ibaraki Agricultural Center, for kindly providing powdery mildew. We also thank Drs. Y. Yoshioka and K. Yamashita for their support in statistical analysis.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Nobuko Fukino
    • 1
    Email author
  • Takayoshi Ohara
    • 1
  • Antonio J. Monforte
    • 2
  • Mitsuhiro Sugiyama
    • 1
  • Yoshiteru Sakata
    • 1
  • Miyuki Kunihisa
    • 1
  • Satoru Matsumoto
    • 1
  1. 1.National Institute of Vegetable and Tea Science (NIVTS)TsuJapan
  2. 2.IRTA Centro de Recerca en AgrigenómicaCabrilsSpain

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