Theoretical and Applied Genetics

, 117:1281

Long distance pollen-mediated flow of herbicide resistance genes in Lolium rigidum

  • Roberto Busi
  • Qin Yu
  • Robert Barrett-Lennard
  • Stephen Powles
Original Paper

DOI: 10.1007/s00122-008-0862-8

Cite this article as:
Busi, R., Yu, Q., Barrett-Lennard, R. et al. Theor Appl Genet (2008) 117: 1281. doi:10.1007/s00122-008-0862-8

Abstract

Gene flow promotes genetic exchange among plant populations mediating evolutionary dynamics; yet, the importance of gene flow at distance via pollen movement is poorly understood. A field experiment at the landscape level was conducted with Lolium rigidum herbicide-susceptible individuals (population VLR1) placed into an otherwise Lolium-free bushland environment at increasing distances from adjacent large commercial crop fields infested with herbicide-resistant L. rigidum. Herbicide resistance was used as a marker to quantify the distance and the rate of pollen-mediated gene flow. About 21,245 seeds were produced on the isolated, susceptible mother plants of which 3,303 seedlings were tested for herbicide resistance and 664 seedlings were found to be resistant. Pollen-mediated gene flow occurred at 3,000 m (maximum tested distance). Both Mendelian and molecular analyses (sequencing and CAPS markers) confirmed the introgression of herbicide resistance genes. This is the first documented case of long-distance gene flow in L. rigidum. The results are important for future modeling simulations of herbicide resistance evolution and subsequent mobility. The adoption of integrated agronomic strategies, the control of potential receptor plants on fields’ margins and conservative use of herbicides can be realistic options to minimize herbicide resistance spread.

Supplementary material

122_2008_862_MOESM1_ESM.doc (139 kb)
Partial sequencing chromatogram of ALS fragment amplified by primer pair ALS197F/ALS197R and ALS574F/ALS574R from resistant plants of progeny P1. Heterozygosity at the Pro197 codon and the Trp574 codon is arrowed. Y: nucleotide C/T, K: G/T. (DOC 139 kb)

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Roberto Busi
    • 1
  • Qin Yu
    • 1
  • Robert Barrett-Lennard
    • 1
  • Stephen Powles
    • 1
  1. 1.Western Australian Herbicide Resistance Initiative, School of Plant Biology, Faculty of Natural and Agricultural SciencesThe University of Western AustraliaCrawleyAustralia

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