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Theoretical and Applied Genetics

, 117:1213 | Cite as

Construction of SSR-based chromosome map in bunching onion (Allium fistulosum)

  • Hikaru Tsukazaki
  • Ken-ichiro Yamashita
  • Shigenori Yaguchi
  • Shinichi Masuzaki
  • Hiroyuki Fukuoka
  • Junichi Yonemaru
  • Hiroyuki Kanamori
  • Izumi Kono
  • Tran Thi Minh Hang
  • Masayoshi Shigyo
  • Akio Kojima
  • Tadayuki Wako
Original Paper

Abstract

We have constructed a linkage map of bunching onion (Allium fistulosum L., 2= 16) using an F2 population of 225 plants. The map consists of 17 linkage groups with 212 bunching onion SSR markers and 42 bulb onion (A. cepa L.) SSR, InDel, CAPS or dCAPS markers, covering 2,069 cM. This is the first report of a linkage map mainly based on SSR markers in the genus Allium. With the 103 anchor markers [81 bunching onion SSRs, 11 bulb onion SSRs and 11 bulb onion non-SSRs (1 InDel, 9 CAPSs and 1 dCAPS)] whose chromosome assignments were identified in A. cepa and/or A. fistulosum, via the use of several kinds of Allium alien addition lines, 16 of the 17 linkage groups were connected to the 8 basic chromosomes of A. cepa.

Keywords

Linkage Group Simple Sequence Repeat Marker Cleave Amplify Polymorphic Sequence Bulb Onion Cleave Amplify Polymorphic Sequence Marker 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We are grateful to Ms. K. Tanaka, K. Wakita and S. Negoro for the technical assistance. This work was supported by the project “Green Technology Project (DM-1604)”, Ministry of Agriculture, Forestry and Fisheries, Japan and Grant-in-Aid for Young Scientists (B) (19780010), Japan Society for the Promotion of Science. The authors wish to thank Ms. N. Matsubara, Y. Kousabara and M. Anraku (former students in Yamaguchi University) for their contributions to this study.

Supplementary material

122_2008_849_MOESM1_ESM.pdf (97 kb)
Supplementary Tables (PDF 97.3 kb)

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Hikaru Tsukazaki
    • 1
  • Ken-ichiro Yamashita
    • 1
  • Shigenori Yaguchi
    • 2
  • Shinichi Masuzaki
    • 2
    • 3
  • Hiroyuki Fukuoka
    • 1
  • Junichi Yonemaru
    • 4
    • 5
  • Hiroyuki Kanamori
    • 6
  • Izumi Kono
    • 6
  • Tran Thi Minh Hang
    • 2
    • 7
  • Masayoshi Shigyo
    • 2
  • Akio Kojima
    • 1
  • Tadayuki Wako
    • 1
  1. 1.National Institute of Vegetable and Tea Science (NIVTS)NAROMieJapan
  2. 2.Department of Biological and Environmental Science, Faculty of AgricultureYamaguchi UniversityYamaguchiJapan
  3. 3.Forensic Science LaboratoryYamaguchi Pref. Police HeadquartersYamaguchiJapan
  4. 4.National Agricultural Research Center for Tohoku Region (NARCT)NAROIwateJapan
  5. 5.QTL Genomics Research CenterNational Institute of Agrobiological Sciences (NIAS)IbarakiJapan
  6. 6.Institute of the Society for Techno-innovation of Agriculture, Forestry and Fisheries (STAFF)IbarakiJapan
  7. 7.Department of Horticulture, Faculty of AgronomyHanoi Agricultural UniversityHanoiVietnam

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