Theoretical and Applied Genetics

, Volume 117, Issue 5, pp 759–767 | Cite as

Mapping of isolate-specific QTLs for clubroot resistance in Chinese cabbage (Brassica rapa L. ssp. pekinensis)

  • Koji SakamotoEmail author
  • Atsuo Saito
  • Nobuaki Hayashida
  • Goro Taguchi
  • Etsuo Matsumoto
Original Paper


A number of clubroot resistant (CR) Chinese cabbage cultivars have been developed in Japan using resistant genes from CR European fodder turnips (B. rapa ssp. rapifera). Clubroot resistance in European fodder turnips are known to be controlled by the combined action of several dominant resistance genes. We have developed three Chinese cabbage clubroot-resistant doubled haploid (DH) lines—T136-8, K10, and C9—which express resistance in different manners against two isolates of Plasmodiophora brassicae, M85 and K04. Depending on the isolates, we identified two CR loci, CRk and CRc. CRk was identified by quantitative trait loci (QTL) analysis of an F2 population derived from a cross between K10 and Q5. This locus showed resistance to both isolates and is located close to Crr3 in linkage group R3. The other locus, CRc was identified by QTL analysis of an F2 population derived from a cross between C9 and susceptible DH line, 6R. This locus was mapped to linkage group R2 and is independent from any published CR loci. We developed sequence-tagged site markers linked to this locus.


Quantitative Trait Locus Amplify Fragment Length Polymorphism Simple Sequence Repeat Marker Double Haploid Chinese Cabbage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors thank Dr. S. Matsumoto of National Institute of Vegetable and Tea Science (NIVTS) for offering information on the SSR markers of B. rapa (BRMS).


  1. Basten CJ, Weir BS, Zeng ZB (2005) QTL cartographer: a reference manual and tutorial for QTL mapping. North Carolina State University, Department of Statistics, RaleighGoogle Scholar
  2. Buczacki ST, Toxopeus H, Mattusch P, Johnston TD, Dixon GR, Hobolth LA (1975) Study of physiologic specialization in Plasmodiophora brassicae: proposals for attempted rationalization through an international approach. Trans Br Mycol Soc 65:295–303CrossRefGoogle Scholar
  3. Crute IR, Gray AR, Crisp P, Buczacki ST (1980) Variation in Plasmodiophora brassicea and resistance to clubroot disease in Brassica and allied crops. A critical review. Plant Breed Abstr 50:91–104Google Scholar
  4. Hayashida N, Takabatake Y, Nakazawa N, Aruga D, Nakanishi H, Taguchi G, Sakamoto K, Matsumoto E (2008) Construction of a practical SCAR marker linked to clubroot resistance in Chinese cabbage, with intensive analysis of HC352b genes. J Jpn Soc Hortic Sci 77:150–154CrossRefGoogle Scholar
  5. Hirai M (2006) Genetics analysis of clubroot resistance in Brassica crops. Breed Sci 56:223–229CrossRefGoogle Scholar
  6. Hirai M, Harada T, Kubo N, Tsukada M, Suwabe K, Matsumoto S (2004) A nobel locus for clubroot resistance in Brassica rapa and its linkage markers. Theor Appl Genet 108:639–643PubMedCrossRefGoogle Scholar
  7. Kim JS, Chung TY, King GJ, Jin M, Yang TJ, Jin YM, Kim HI, Park BS (2006) A sequence-tagged linkage map of Brassica rapa. Genetics 174:29–39PubMedCrossRefGoogle Scholar
  8. Kosambi DD (1944) The estimation of map distance from recombination values. Ann Eugen 12:172–175Google Scholar
  9. Kuginuki Y, Ajisaka H, Yui M, Yoshikawa H, Hida K, Hirai M (1997) RAPD markers linked to a clubroot-resistance locus in Brassica rapa L. Euphytica 98:149–154CrossRefGoogle Scholar
  10. Kuginuki Y, Yoshikawa H, Hirai M (1999) Variation in virulence of Plasmodiophora brassicea in Japan tested with clubroot-resistant cultivars of Chinese cabbage (Brassica rapa L. ssp. pekinensis). Eur J Plant Pathol 105:327–332CrossRefGoogle Scholar
  11. Lowe AJ, Moule C, Trick M, Edwards KJ (2004) Efficient large-scale development of microsatellites for marker and mapping applications in Brassica crop species. Theor Appl Genet 108:1103–1112PubMedCrossRefGoogle Scholar
  12. Manzanares-Dauleux MJ, Delourme R, Baron F, Thomas G (2000) Mapping of one major gene and of QTLs involved in resistance to clubroot in Brassica napus. Theor Appl Genet 101:885–891CrossRefGoogle Scholar
  13. Matsumoto E, Yasui C, Ohi M, Tsukada M (1998) Linkage analysis of RFLP markers for clubroot resistance and pigmentation in Chinese cabbage (Brassica rapa ssp. pekinensis). Euphytica 104:79–86CrossRefGoogle Scholar
  14. Matsumoto E, Hayashida N, Sakamoto K, Ohi M (2005) Behavior of DNA markers linked to a clubroot resistance gene in segregating populations of Chinese cabbage (Brassica rapa ssp. pekinensis). J Jpn Soc Hortic Sci 74(5):367–373CrossRefGoogle Scholar
  15. Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832PubMedCrossRefGoogle Scholar
  16. Piao ZY, Deng YQ, Choi SR, Park YJ, Lim YP (2004) SCAR and CAPS mapping of CRb, a gene conferring resistance to Plasmodiophora brassicae in Chinese cabbage (Brassica rapa ssp. pekinensis). Theor Appl Genet 108:1458–1465PubMedCrossRefGoogle Scholar
  17. Rocherieux J, Glory P, Giboulot A, Boury S, Barbeyron G, Thomas G, Manzanares-Dauleux MJ (2004) Isolation-specific and broad-spectrum QTLs are involved in the control of clubroot in Brassica oleracea. Theor Appl Genet 108:1555–1563PubMedCrossRefGoogle Scholar
  18. Saito M, Kubo N, Matsumoto S, Suwabe K, Tsukada M, Hirai M (2006) Fine mapping of the clubroot resistance gene, Crr3, in Brassica rapa. Theor Appl Genet 114:81–91PubMedCrossRefGoogle Scholar
  19. Sakamoto K, Nishio T (2001) Distribution of S haplotypes in commercial cultivars of Brassica rapa. Plant Breed 120:155–161CrossRefGoogle Scholar
  20. Suwabe K, Ikehashi H, Nunome T, Kage T, Hirai M (2002) Isolation and characterization of microsatellites in Brassica rapa L. Theor Appl Genet 104:1092–1098PubMedCrossRefGoogle Scholar
  21. Suwabe K, Tsukazaki H, Iketani H, Hatakeyama K, Fujimura M, Nunome T, Fukuoka H, Matsumoto S, Hirai M (2003) Identification of two loci for resistance to clubroot (Plasmodiophora brassicae Woronin) in Brassica rapa L. Theor Appl Genet 107:997–1002PubMedCrossRefGoogle Scholar
  22. Suwabe K, Tsukazaki H, Iketani H, Hatakeyama K, Kondo M, Fujimura M, Nunome T, Fukuoka H, Hirai M, Matsumoto S (2006) Simple sequence repeat-based comparative genomics between Brassica rapa and Arabidopsis thaliana: the genetic origin of clubroot resistance. Genetics 173:309–319PubMedCrossRefGoogle Scholar
  23. Tjallingii F (1965) Testing clubroot-resistance of turnips in the Netherlands and the physiologic specialization of Plasmodiophora brassicae. Euphytica 14:1–22Google Scholar
  24. Toxopeus H, Janssen AMP (1975) Clubroot resistance in turnip II. The slurry screening method and clubroot races in the Netherlands. Euphytica 24:751–755CrossRefGoogle Scholar
  25. Williams PH (1966) A system for the determination of races of Plasmodiophora brassicae that infect cabbage and rutabaga. Phytopathology 56:624–626Google Scholar
  26. Wit F (1964) Inheritance of reaction to clubroot in turnips. Hortic Res 5:47–49Google Scholar
  27. Wit F, Van De Weg M (1964) Clubroot-resistance in turnips (Brassica campestris L.). I. Physiologic races of the parasite and their identification in mixtures. Euphytica 13:9–18CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Koji Sakamoto
    • 1
    Email author
  • Atsuo Saito
    • 1
  • Nobuaki Hayashida
    • 2
  • Goro Taguchi
    • 3
  • Etsuo Matsumoto
    • 4
  1. 1.Takii Plant Breeding and Experiment StationKonanJapan
  2. 2.Division of Gene ResearchShinshu UniversityUedaJapan
  3. 3.Faculty of Textile Science and TechnologyShinshu UniversityUedaJapan
  4. 4.Nagano Vegetable and Ornamental Crops Experiment StationMatsushiroJapan

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