Molecular mapping of soybean rust (Phakopsora pachyrhizi) resistance genes: discovery of a novel locus and alleles

  • Alexandre Garcia
  • Éberson Sanches Calvo
  • Romeu Afonso de Souza Kiihl
  • Arlindo Harada
  • Dario Minoru Hiromoto
  • Luiz Gonzaga Esteves Vieira
Original Paper

Abstract

Soybean production in South and North America has recently been threatened by the widespread dissemination of soybean rust (SBR) caused by the fungus Phakopsora pachyrhizi. Currently, chemical spray containing fungicides is the only effective method to control the disease. This strategy increases production costs and exposes the environment to higher levels of fungicides. As a first step towards the development of SBR resistant cultivars, we studied the genetic basis of SBR resistance in five F2 populations derived from crossing the Brazilian-adapted susceptible cultivar CD 208 to each of five different plant introductions (PI 200487, PI 200526, PI 230970, PI 459025, PI 471904) carrying SBR-resistant genes (Rpp). Molecular mapping of SBR-resistance genes was performed in three of these PIs (PI 459025, PI 200526, PI 471904), and also in two other PIs (PI 200456 and 224270). The strategy mapped two genes present in PI 230970 and PI 459025, the original sources of Rpp2 and Rpp4, to linkage groups (LG) J and G, respectively. A new SBR resistance locus, rpp5 was mapped in the LG-N. Together, the genetic and molecular analysis suggested multiple alleles or closely linked genes that govern SBR resistance in soybean.

Notes

Acknowledgments

The authors would like to thank Marcelo S. Baço and Adriano A. Marino for technical assistance with plant analysis and the anonymous reviewers who contributed to improvement of the manuscript. This project was financially supported by FAPEMAT (Fundação de Apoio a Pesquisa do Estado de Mato Grosso) and FINEP (Financiadora de Estudos e Projetos – Ação Estruturante Transversal). Alexandre Garcia received a scholarship from Fundação Araucária.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Alexandre Garcia
    • 1
    • 2
  • Éberson Sanches Calvo
    • 2
  • Romeu Afonso de Souza Kiihl
    • 2
  • Arlindo Harada
    • 2
  • Dario Minoru Hiromoto
    • 3
  • Luiz Gonzaga Esteves Vieira
    • 4
  1. 1.State University of Londrina (UEL)LondrinaBrazil
  2. 2.Tropical Melhoramento e Genética Ltda (TMG)CambéBrazil
  3. 3.Fundação de Apoio a Pesquisa Agropecuária do Estado de Mato Grosso (Fundação MT)RondonópolisBrazil
  4. 4.Instituto Agronômico do Paraná (IAPAR)LondrinaBrazil

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