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Theoretical and Applied Genetics

, Volume 117, Issue 4, pp 523–529 | Cite as

Exploiting synteny in Cucumis for mapping of Psm: a unique locus controlling paternal mitochondrial sorting

  • Sulieman Al-Faifi
  • Jenelle D. F. Meyer
  • Jordi Garcia-Mas
  • Antonio J. Monforte
  • Michael J. HaveyEmail author
Original Paper

Abstract

The three genomes of cucumber show different modes of transmission, nuclear DNA bi-parentally, plastid DNA maternally, and mitochondrial DNA paternally. The mosaic (MSC) phenotype of cucumber is associated with mitochondrial DNA rearrangements and is a valuable tool for studying mitochondrial transmission. A nuclear locus (Psm) has been identified in cucumber that controls sorting of paternally transmitted mitochondrial DNA. Comparative sequencing and mapping of cucumber and melon revealed extensive synteny on the recombinational and sequence levels near Psm and placed this locus on linkage group R of cucumber and G10 of melon. However, the cucumber genomic region near Psm was surprisingly monomorphic with an average of one SNP every 25 kb, requiring that a family from a more diverse cross is produced for fine mapping and eventual cloning of Psm. The cucumber ortholog of Arabidopsis mismatch repair (MSH1) was cloned and it segregated independently of Psm, revealing that this candidate gene is not Psm.

Keywords

Single Nucleotide Polymorphism Linkage Group Melon Simple Sequence Repeat Marker Plant Introduction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Sulieman Al-Faifi
    • 1
    • 2
  • Jenelle D. F. Meyer
    • 1
  • Jordi Garcia-Mas
    • 3
  • Antonio J. Monforte
    • 3
  • Michael J. Havey
    • 4
    Email author
  1. 1.Department of HorticultureUniversity of WisconsinMadisonUSA
  2. 2.Department of Plant ProductionCollege of Food Sciences and Agriculture, King Saud UniversityRiyadhSaudi Arabia
  3. 3.Centre de Recerca en Agrigenòmica CSIC-IRTA-UABIRTACabrils (Barcelona)Spain
  4. 4.USDA/ARS, Department of HorticultureUniversity of WisconsinMadisonUSA

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