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QTL identification and microphenotype characterisation of the developmentally regulated yellow rust resistance in the UK wheat cultivar Guardian

  • J. P. E. Melichar
  • S. Berry
  • C. Newell
  • R. MacCormack
  • L. A. Boyd
Original Paper

Abstract

Yellow rust (causal agent: Puccinia striiformis f.sp. tritici) resistance in the UK wheat cultivar Guardian is developmentally regulated, resistance increasing as the plant matures. Yellow rust resistance was assessed under field conditions on plants after ear emergence to ensure maximum expression of resistance. Three quantitative trait loci (QTL) for yellow rust resistance were identified, being located on chromosomes 1B (QPst.jic-1B), 2D (QPst.jic-2D) and 4B (QPst.jic-4B). The largest resistance effect, QPst.jic-1B located to the same position on the long arm of chromosome 1B as the known durable source of yellow rust resistance, Yr29. Microscopic studies were carried out to determine what effect the resistance in Guardian had on the development of P. striiformis f.sp. tritici. While the adult plant resistance in Guardian did not prevent germinated urediniospores from establishing an effective infection site, the growth of hyphae within flag leaf tissue was significantly inhibited, slowing the development of microcolonies. 3,3-diaminabenzadine (DAB) and trypan blue staining indicated that this inhibition of hyphal growth was not associated with hydrogen peroxide accumulation or extensive plant cell death.

Keywords

Durable resistance Puccinia striiformis QTL mapping Stripe rust Triticum aestivum 

Notes

Acknowledgments

This work was supported in part by a CASE PhD studentship funded by BBSRC and Nickerson-Advanta, UK, and by European Union funding through the EU FP6 Integrated Project, BioExploit.

References

  1. Bayles RA, Flath K, Hövmoller MS, de Vallavieille-Pope C (2000) Breakdown of the Yr17 resistance to yellow rust of wheat in northern Europe. Agronomie 20:805–811CrossRefGoogle Scholar
  2. Boyd LA (2005) Centenary review: can Robigus defeat an old enemy?—Yellow rust of wheat. J Agric Sci 143:233–243CrossRefGoogle Scholar
  3. Boyd LA (2006) Perspective: can the durability of resistance be predicted? J Sci Food Agric 86:2523–2526CrossRefGoogle Scholar
  4. Boyd LA, Minchin PN (2001) Wheat mutants showing altered adult plant disease resistance. Euphytica 122:361–368CrossRefGoogle Scholar
  5. Boyd LA, Smith PH, Wilson AH, Minchin PN (2002) Mutations in wheat showing altered field resistance to yellow and brown rust. Genome 45:1035–1040PubMedCrossRefGoogle Scholar
  6. Broers LHM, Cuesta Subias X, López Atilano RM (1996) Field assessment of quantitative resistance to yellow rust in ten spring bread wheat cultivars. Euphytica 90:9–16Google Scholar
  7. Bryan GJ, Collins AJ, Stephenson P, Orry A, Smith JB, Gale MD (1997) Isolation and characterisation of microsatellites from hexaploid bread wheat. Theor Appl Genet 94:557–563CrossRefGoogle Scholar
  8. Cartwright DW, Russell GE (1981) Development of Puccinia striiformis in a susceptible winter wheat variety. Trans Br Mycol Society 76:197–204Google Scholar
  9. Garrood JM (2001) The interaction of Puccinia striiformis with wheat and barley. PhD thesis, University of East AngliaGoogle Scholar
  10. Genstat 5 Committee (2005) Genstat for Windows Release 8.2. VSN International. Wilkinson House, OxfordGoogle Scholar
  11. Goddard MV (1976) Cytological studies of Puccinia striiformis (yellow rust of wheat). Trans Br Mycol Soc 66:433–437CrossRefGoogle Scholar
  12. Görg R, Hollricher K, Schulze-Lefert P (1993) Functional analysis and RFLP mediated mapping of the Mlg resistance locus in barley. Plant J. 3:857–866CrossRefGoogle Scholar
  13. Kolmer JA (1996) Genetics of resistance to wheat leaf rust. Ann Rev Phytopathol 34:435–445CrossRefGoogle Scholar
  14. Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:173–175Google Scholar
  15. Lamb C, Dixon RA (1997) The oxidative burst in plant disease resistance. Ann Rev Plant Physiol Plant Mol Biol 48:251–275CrossRefGoogle Scholar
  16. Ma H, Singh RP (1996) Expression of adult plant resistance to stripe rust at different growth stages of wheat. Plant Dis 80:375–379Google Scholar
  17. Mares DJ (1979) Microscopic study of the development of yellow rust (Puccinia striiformis) in a wheat cultivar showing adult plant resistance. Physiol Plant Pathol 15:289–296CrossRefGoogle Scholar
  18. Mares DJ, Cousen S (1977) The interaction of yellow rust (Puccinia striiformis) with winter wheat cultivars showing adult plant resistance: macroscopic and microscopic events associated with the resistance reaction. Physiol Plant Pathol 10:257–274Google Scholar
  19. McIntosh RA, Wellings CR, Park RF (1995) Wheat rusts: an atlas of resistance genes. Kluwer, DordrechtGoogle Scholar
  20. McNeal FH, Konzak CF, Smith EP, Tate WS, Russell TS (1971) A uniform system for recording and processing cereal research data. U.S. Dept. of Agriculture, Agricultural research service, Montana Agricultural Experimental Station 135:34–121Google Scholar
  21. Moldenhauer J, Moerschbacher BM, van der Westhuizen AJ (2006) Histological investigation of stripe rust (Puccinia striiformis f.sp. tritici) development in resistant and susceptible wheat cultivars. Plant Pathol 55:469–474CrossRefGoogle Scholar
  22. Moldenhauer J, Pretorius ZA, Moerschbacher BM, Prins R, van der Westhuizen AJ (2007) Histopathology and PR-protein markers provide insight into adult plant resistance to stripe rust of wheat. Molecular Plant Pathol 9:137–145CrossRefGoogle Scholar
  23. Murray GM, Ellison PJ, Watson A (1995) Effects of stripe rust on the wheat plant. Australas Plant Pathol 24:261–270CrossRefGoogle Scholar
  24. Pretorius ZA, Rijkenberg FHJ, Wilcoxson RD (1988) Efects of growth stage, leaf position and temperature on adult-plant resistance of wheat infected by Puccinia recondita f.sp. tritici. Plant Pathol 37:36–44CrossRefGoogle Scholar
  25. Ramburan VP, Pretorius ZA, Louw JH, Boyd LA, Smith PH, Boshoff WHP, Prins R (2004) A genetic analysis of adult plant resistance to stripe rust in the wheat cultivar Kariega. Theor Appl Genet 108:1426–1433PubMedCrossRefGoogle Scholar
  26. Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier M-H, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023PubMedGoogle Scholar
  27. Rosewarne GM, Singh RP, Huerta-Espino J, William HM, Bouchet S, Cloutier S, McFadden H, Lagudah ES (2006) Leaf tip necrosis, molecular markers and ß1-proteasome subunits associated with the slow rusting resistance genes Lr46/Yr29. Theor Appl Genet 112:500–508PubMedCrossRefGoogle Scholar
  28. Singh RP, Huerta-Espino J, Rajaram S (2000) Achieving near-immunity to leaf and stripe rust in wheat by combining slow rusting resistance genes. Acta Phytopathol Entomol Hung 35:133–139Google Scholar
  29. Singh RP, Huerto-Espino J, William M (2001) Slow rusting gene based resistance to leaf and yellow rust in wheat: genetics and breeding at CIMMYT. In: Proceedings of the 10th Assembly of the Wheat Breeding Society of Australia Inc., Mildura, Australia, 16–21 September 2001. Breeding Society of Australia Inc., Australia, pp 103–108Google Scholar
  30. Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114PubMedCrossRefGoogle Scholar
  31. Stubbs RW, Prescott JM, Saari EE, Dubin HJ (1986) Cereal disease methodology manual. CIMMYT, Mexico, p 46Google Scholar
  32. Thordal-Christensen H, Zhang Z, Wei Y, Collinge DB (1997) Subcellular localization of H2O2 in plants: H2O2 accumulation in papillae and hypersensitive response during the barley-powdery mildew interaction. Plant J 11:1187–1194CrossRefGoogle Scholar
  33. van Ooijen JW (2004) MapQTL® 5. Software for the mapping of quantitative trait loci in experimental populations. Kyazma B.V, WageningenGoogle Scholar
  34. van Ooijen JW, Voorips RE (2001) JoinMap® 3.0. Software for the calculation of genetic linkage maps. Plant Research International, WageningenGoogle Scholar
  35. William HM, Singh RP, Huerta-Espino J, Ortiz Islas S, Hoisington D (2003) Molecular marker mapping of leaf rust resistance gene Lr46 and its association with stripe rust resistance gene Yr29 in wheat. Phytopathol 93:153–159CrossRefGoogle Scholar
  36. Zadoks JC, Chang TT, Konzak CF (1974) A decimal code for the growth stages of cereals. Weed Res 14:415–421CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • J. P. E. Melichar
    • 1
    • 2
  • S. Berry
    • 3
  • C. Newell
    • 3
  • R. MacCormack
    • 1
  • L. A. Boyd
    • 1
  1. 1.Department of Disease and Stress BiologyJohn Innes CentreNorwichUK
  2. 2.Syngenta Seeds LtdWhittlesfordUK
  3. 3.Nickerson-Advanta UKDockingUK

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