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Segregation distortion in Lolium: evidence for genetic effects

  • U. C. M. Anhalt
  • P. (J. S.) Heslop-Harrison
  • S. Byrne
  • A. Guillard
  • S. BarthEmail author
Original Paper

Abstract

Segregation distortion (SD) is the deviation of genetic segregation ratios from their expected Mendelian fraction and is a common phenomenon found in most genetic mapping studies. In this study two segregating Lolium perenne populations were used to construct two genetic maps: an ‘F2 biomass’ consisting of 360 genotypes and an ‘F1 late flowering’ sibling based population consisting of 182 genotypes. Additionally two parental maps were generated for the ‘F1 late flowering’ population. SD was detected and p-values for SD were calculated for each marker locus. The ‘F1 late flowering’ map had only half of the extent of SD (32%) compared to the map based on the ‘F2 biomass’ population (63%). Molecular marker data have been supplemented with genomic in situ hybridization (GISH) data to show non major non-recombined segments of Fescue chromosomes within the parental inbred ryegrass lines with a Festuca × Lolium pedigree. We conclude that SD in our study is more likely caused by genetic effects rather than by population structure and marker types. Two new L. perenne mapping populations including their genetic maps are introduced; one of them is the largest reported Lolium mapping population consisting of 360 individuals.

Keywords

Linkage Group Amplify Fragment Length Polymorphism Mapping Population Simple Sequence Repeat Marker Cytoplasmic Male Sterility 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

UCMA was financed under a Teagasc Walsh Fellowship. We acknowledge the National Development Plan for financial contributions. We are grateful for the help of Ms. Kim Tögemann, Ms. Evelyn Fricke, Ms. Maryline Vernet and Ms. Rosanna Hennessy in DNA extractions and genotyping.

Supplementary material

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • U. C. M. Anhalt
    • 1
    • 2
  • P. (J. S.) Heslop-Harrison
    • 2
  • S. Byrne
    • 1
  • A. Guillard
    • 1
  • S. Barth
    • 1
    Email author
  1. 1.Teagasc Crops Research CentreCarlowIreland
  2. 2.Department of BiologyUniversity of LeicesterLeicesterUK

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