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Theoretical and Applied Genetics

, Volume 117, Issue 1, pp 11–18 | Cite as

Fine mapping of a recessive genic male sterility gene (Bnms3) in rapeseed (Brassica napus) with AFLP- and Arabidopsis-derived PCR markers

  • Junping He
  • Liping Ke
  • Dengfeng Hong
  • Yanzhou Xie
  • Guichun Wang
  • Pingwu Liu
  • Guangsheng Yang
Original paper

Abstract

9012AB, a recessive genic male sterility (RGMS) line developed from spontaneous mutation in Brassica napus (Chen et al. in Acta Agron Sin 24:431–438, 1998), has been playing an increasing role in hybrid cultivar development in China. The male sterility of 9012AB is controlled by two recessive genes (designated Bnms3 and Bnms4) interacting with one recessive epistatic suppressor gene (esp). Previous study has identified seven AFLP markers, six of which were co-segregated with the Bnms3 gene in a small population (Ke et al. in Plant Breed 124:367–370, 2005). By cloning these AFLP markers and their flanking sequences, five of the six co-segregated markers were successfully converted into sequence characterized amplified region (SCAR) markers. For fine mapping of the Bnms3 gene, these SCAR markers were analyzed in a NIL population of 4,136 individuals. The Bnms3 gene was then genetically mapped to a region of 0.56 cM, with 0.15 cM from marker SEP8 and 0.41 from marker SEP4, respectively. BLAST analysis with these SCAR marker sequences identified a collinear genomic region in Arabidopsis chromosome 5, from which two specific PCR markers further narrowed the Bnms3 locus from an interval of 0.56 to 0.14 cM. These results provide additional information for map-based cloning of the Bnms3 gene and will be helpful for marker-assisted selection (MAS) of elite RGMS lines and maintainers.

Keywords

Male Sterility Cytoplasmic Male Sterility AFLP Marker Sequence Characterize Amplify Region Marker Arabidopsis Chromosome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This research was supported by National Natural Science Foundation of China (30670123), National “973” Project (No. 2007CB109006), National “863” Project (2006AA10Z1B8), and Program for Changjiang Scholars and Innovation Research Team in University (No. IRT0442).

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Junping He
    • 1
  • Liping Ke
    • 1
    • 2
  • Dengfeng Hong
    • 1
  • Yanzhou Xie
    • 1
  • Guichun Wang
    • 1
  • Pingwu Liu
    • 1
  • Guangsheng Yang
    • 1
  1. 1.National Key Laboratory of Crop Genetic Improvement, National Center of Rapeseed Improvement (Wuhan Branch)Huazhong Agricultural UniversityWuhanChina
  2. 2.Institute of Virology and BiotechnologyZhejiang Academy of Agricultural ScienceHangzhouChina

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