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Theoretical and Applied Genetics

, Volume 116, Issue 8, pp 1155–1166 | Cite as

The adult plant rust resistance loci Lr34/Yr18 and Lr46/Yr29 are important determinants of partial resistance to powdery mildew in bread wheat line Saar

  • M. Lillemo
  • B. Asalf
  • R. P. Singh
  • J. Huerta-Espino
  • X. M. Chen
  • Z. H. He
  • Å. Bjørnstad
Original Paper

Abstract

Powdery mildew, caused by Blumeria graminis f. sp. tritici is a major disease of wheat (Triticum aestivum L.) that can be controlled by resistance breeding. The CIMMYT bread wheat line Saar is known for its good level of partial and race non-specific resistance, and the aim of this study was to map QTLs for resistance to powdery mildew in a population of 113 recombinant inbred lines from a cross between Saar and the susceptible line Avocet. The population was tested over 2 years in field trials at two locations in southeastern Norway and once in Beijing, China. SSR markers were screened for association with powdery mildew resistance in a bulked segregant analysis, and linkage maps were created based on selected SSR markers and supplemented with DArT genotyping. The most important QTLs for powdery mildew resistance derived from Saar were located on chromosomes 7DS and 1BL and corresponded to the adult plant rust resistance loci Lr34/Yr18 and Lr46/Yr29. A major QTL was also located on 4BL with resistance contributed by Avocet. Additional QTLs were detected at 3AS and 5AL in the Norwegian testing environments and at 5BS in Beijing. The population was also tested for leaf rust (caused by Puccinia triticina) and stripe rust (caused by P. striiformis f. sp. tritici) resistance and leaf tip necrosis in Mexico. QTLs for these traits were detected on 7DS and 1BL at the same positions as the QTLs for powdery mildew resistance, and confirmed the presence of Lr34/Yr18 and Lr46/Yr29 in Saar. The powdery mildew resistance gene at the Lr34/Yr18 locus has recently been named Pm38. The powdery mildew resistance gene at the Lr46/Yr29 locus is designated as Pm39.

Keywords

Quantitative Trait Locus Powdery Mildew Simple Sequence Repeat Marker Leaf Rust Quantitative Trait Locus Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by grants from the Norwegian Research Council, The International Collaboration Project from Ministry of Agriculture of China (2006-G2), and GRDC, Australia. The helpful assistance of Anne Guri Marøy with molecular marker genotyping and Yalew Tarkegne for the field trials is greatly acknowledged. We also thank Alireza Navabi for providing the seed of the Avocet × Saar population that was used to produce the F6 lines used in the study, and Martin Ganal at TraitGenetics Inc., Gatersleben, Germany and Atushi Torada at Hokuren Agricultural Research Institute, Hokkaido, Japan for providing unpublished primer sequences.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • M. Lillemo
    • 1
  • B. Asalf
    • 1
  • R. P. Singh
    • 2
  • J. Huerta-Espino
    • 3
  • X. M. Chen
    • 4
  • Z. H. He
    • 4
    • 5
  • Å. Bjørnstad
    • 1
  1. 1.Department of Plant and Environmental SciencesNorwegian University of Life SciencesÅsNorway
  2. 2.CIMMYTMéxicoMexico
  3. 3.Campo Experimental Valle de Mexico-INIFAPChapingo, Edo. de MexicoMexico
  4. 4.Institute of Crop Sciences/National Wheat Improvement CenterChinese Academy of Agricultural SciencesBeijingPeople’s Republic of China
  5. 5.CIMMYT China Officec/o Chinese Academy of Agricultural SciencesBeijingPeople’s Republic of China

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