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Theoretical and Applied Genetics

, Volume 116, Issue 6, pp 881–890 | Cite as

Genetic dissection and pyramiding of quantitative traits for panicle architecture by using chromosomal segment substitution lines in rice

  • Tsuyu Ando
  • Toshio Yamamoto
  • Takehiko Shimizu
  • Xiu Fang Ma
  • Ayahiko Shomura
  • Yoshinobu Takeuchi
  • Shao Yang Lin
  • Masahiro Yano
Original Paper

Abstract

To understand the genetic basis of yield-related traits of rice, we developed 39 chromosome segment substitution lines (CSSLs) from a cross between an average-yielding japonica cultivar, Sasanishiki, as the recurrent parent and a high-yielding indica cultivar, Habataki, as the donor. Five morphological components of panicle architecture in the CSSLs were evaluated in 2 years, and 38 quantitative trait loci (QTLs) distributed on 11 chromosomes were detected. The additive effect of each QTL was relatively small, suggesting that none of the QTLs could explain much of the phenotypic difference in sink size between Sasanishiki and Habataki. We developed nearly isogenic lines for two major QTLs, qSBN1 (for secondary branch number on chromosome 1) and qPBN6 (for primary branch number on chromosome 6), and a line containing both. Phenotypic analysis of these lines revealed that qSBN1 and qPBN6 contributed independently to sink size and that the combined line produced more spikelets. This suggests that the cumulative effects of QTLs distributed throughout the genome form the major genetic basis of panicle architecture in rice.

Keywords

Epistatic Interaction Panicle Length Major QTLs Japonica Cultivar Morphological Component 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The seeds and genotype information of the CSSLs are available from the Rice Genome Resource Center (http://www.rgrc.dna.affrc.go.jp/index.html) of NIAS. This work was supported by a grant from the Ministry of Agriculture, Forestry, and Fisheries of Japan (Green Technology Project DM-1003 and QT-1005).

Supplementary material

122_2008_722_MOESM1_ESM.pdf (129 kb)
(PDF 128 kb)

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Tsuyu Ando
    • 1
  • Toshio Yamamoto
    • 2
  • Takehiko Shimizu
    • 1
  • Xiu Fang Ma
    • 2
    • 3
  • Ayahiko Shomura
    • 1
  • Yoshinobu Takeuchi
    • 1
    • 4
  • Shao Yang Lin
    • 1
    • 5
  • Masahiro Yano
    • 2
  1. 1.Institute of the Society for Techno-Innovation of Agriculture, Forestry, and FisheriesTsukuba, IbarakiJapan
  2. 2.National Institute of Agrobiological SciencesTsukuba, IbarakiJapan
  3. 3.North Japonica Hybrid Rice Research and Development Center, Liaoning Rice Research InstitutionShenyang, LiaoningChina
  4. 4.National Institute of Crop ScienceTsukuba, IbarakiJapan
  5. 5.Honda Research Institute JapanKisarazu, ChibaJapan

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