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Theoretical and Applied Genetics

, Volume 116, Issue 3, pp 313–324 | Cite as

Mapping of adult plant stripe rust resistance genes in diploid A genome wheat species and their transfer to bread wheat

  • Parveen Chhuneja
  • Satinder Kaur
  • Tosh Garg
  • Meenu Ghai
  • Simarjit Kaur
  • M. Prashar
  • N. S. Bains
  • R. K. Goel
  • Beat Keller
  • H. S. Dhaliwal
  • Kuldeep Singh
Original Paper

Abstract

Stripe rust, caused by Puccinia striiformis West. f.sp. tritici, is one of the most damaging diseases of wheat worldwide. Forty genes for stripe rust resistance have been catalogued so far, but the majority of them are not effective against emerging pathotypes. Triticum monococcum and T. boeoticum have excellent levels of resistance to rusts, but so far, no stripe rust resistance gene has been identified or transferred from these species. A set of 121 RILs generated from a cross involving T. monococcum (acc. pau14087) and T. boeoticum (acc. pau5088) was screened for 3 years against a mixture of pathotypes under field conditions. The parental accessions were susceptible to all the prevalent pathotypes at the seedling stage, but resistant at the adult plant stage. Genetic analysis of the RIL population revealed the presence of two genes for stripe rust resistance, with one gene each being contributed by each of the parental lines. A linkage map with 169 SSR and RFLP loci generated from a set of 93 RILs was used for mapping these resistance genes. Based on phenotypic data for 3 years and the pooled data, two QTLs, one each in T. monococcum acc. pau14087 and T. boeoticum acc. pau5088, were detected for resistance in the RIL population. The QTL in T. monococcum mapped on chromosome 2A in a 3.6 cM interval between Xwmc407 and Xwmc170, whereas the QTL from T. boeoticum mapped on 5A in 8.9 cM interval between Xbarc151 and Xcfd12 and these were designated as QYrtm.pau-2A and QYrtb.pau-5A, respectively. Based on field data for 3 years, their R 2 values were 14 and 24%, respectively. T. monococcum acc. pau14087 and three resistant RILs were crossed to hexaploid wheat cvs WL711 and PBW343, using T. durum as a bridging species with the objective of transferring these genes into hexaploid wheat. The B genome of T. durum suppressed resistance in the F1 plants, but with subsequent backcrossing one resistance gene could be transferred from one of the RILs to the hexaploid wheat background. This gene was derived from T. boeoticum acc. pau5088 as indicated by co-introgression of T. boeoticum sequences linked to stripe rust resistance QTL, QYrtb.pau-5A. Homozygous resistant progenies with 40–42 chromosomes have been identified.

Keywords

Rust Resistance Hexaploid Wheat Stripe Rust Leaf Rust Resistance Stripe Rust Resistance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was carried under Indo-Swiss collaboration in Biotechnology. The financial support provided by the Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India, Swiss Agency for Development and Cooperation (SDC; to KS and BK) and the Swiss National Science Foundation (3100-105620 (BK) is gratefully acknowledged. We are thankful to the staff of the wheat breeding section of the department for the rust inoculum.

Supplementary material

References

  1. Aghaee-Sarbarzeh M, Dhaliwal HS, Chhuneja P, Singh H (2001) Suppression of rust resistance genes from distantly related species in Triticum durum-Aegilops amphiploids. Wheat Inf Ser 92:12–16Google Scholar
  2. Bai D, Knott DR (1992) Suppression of rust resistance in bread wheat (Triticum aestivum L.) by D-genome chromosomes. Genome 35:276–282Google Scholar
  3. Börner A, Röder MS, Unger O, Meinel A (2000) The detection and molecular mapping of a major gene for non-specific adult-plant disease resistance against stripe rust (Puccinia striiformis) in wheat. Theor Appl Genet 100:1095–1099CrossRefGoogle Scholar
  4. Bossolini E, Krattinger SG, Keller B (2006) Development of simple sequence repeat markers specific for the Lr34 resistance region of wheat using sequence information from rice and Aegilops tauschii. Theor Appl Genet 113:1049–1062PubMedCrossRefGoogle Scholar
  5. Chague V, Fahima T, Dahan A, Sun GL, Korol AB, Ronin YI, Grama A, Röder MS, Nevo E (1999) Isolation of microsatellite and RAPD markers flanking the Yr15 gene of wheat using NILs and bulked segregant analysis. Genome 42:1050–1056PubMedCrossRefGoogle Scholar
  6. Churchill GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971PubMedGoogle Scholar
  7. Dhaliwal HS, Chhuneja P, Singh I, Ghai M, Goel RK, Garg M, Keller B, Röder M, Singh K (2003) Triticum monococcum—a novel source for transfer and exploitation of disease resistance in wheat. In: Proceedings of the 10th international wheat genetics symposium, Paestum, Italy, pp 346–349Google Scholar
  8. Dhaliwal HS, Singh H, Singh KS, Randhawa HS (1993) Evaluation and cataloguing of wheat germplasm for disease resistance and quality. In: Damania AB (eds) Biodiversity and wheat improvement. Wiley, London, pp 123–140Google Scholar
  9. Dvorak J, diTerlizzi P, Zhang HB, Resta P (1993) The evolution of polyploidy wheats: identification of the A genome donor species. Genome 36:21–31CrossRefPubMedGoogle Scholar
  10. Dyck PL, Bartos P (1994) Attempted transfer of leaf rust resistance from Triticum monococcum and durum wheat to hexaploid wheat. Can J Plant Sci 74:733–736Google Scholar
  11. Feldman M, Sears ER (1981) The wild gene resources of wheat. Sci Am 244:98–109CrossRefGoogle Scholar
  12. Friebe B, Jiang J, Raupp WJ, McIntosh RA, Gill BS (1996) Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status. Euphytica 91:59–87CrossRefGoogle Scholar
  13. Gill RS, Multani DS, Dhaliwal HS (1986) Transfer of isoproturon resistance from T. monococcum to T. durum. Crop Improv 13:200–203Google Scholar
  14. Hussien T, Bowden RL, Gill BS, Cox TS, Marshall DS (1997) Performance of four new leaf rust resistance genes transferred to common wheat from Aegilops tauschii and Triticum monococcum. Plant Dis 81:582–586CrossRefGoogle Scholar
  15. Jiang J, Friebe B, Gill BS (1994) Recent advances in alien gene transfer in wheat. Euphytica 73:199–212CrossRefGoogle Scholar
  16. Johnson R (1988) Durable resistance to yellow (stripe) rust in wheat and its implications in plant breeding. In: Simmonds NW, Rajaram S (eds) Breeding strategies for resistance to the rusts of wheat, CIMMYT, Mexico, pp 63–75Google Scholar
  17. Kema GHJ, Lange W, Van Silfhout CH (1995) Differential suppression of stripe rust resistance in synthetic wheat hexaploids derived from Triticum turgidum subsp. dicoccoides and Aegilops squarrosa. Phytopathology 85:425–429CrossRefGoogle Scholar
  18. Kerber ER (1983) Suppression of rust resistance in amphiploidies of Triticum. In: Sakamoto S (eds) Proceedings of the 6th international wheat genetics symposium, Kyoto, Japan, 28 November–3 December, pp 813–817Google Scholar
  19. Kerber ER, Dyck PL (1973) Inheritance of stem rust resistance transferred from diploid wheat (Triticum monococcum) to tetraploid and hexaploid wheat and chromosome location of the gene involved. Can J Genet Cytol 15:397–409Google Scholar
  20. Kerber ER, Green GJ (1980) Suppression of stem rust resistance in hexaploid wheat cv. Canthatch by chromosome 7DL. Can J Bot 58:1347–1350Google Scholar
  21. Knott DR (2000) Inheritance of resistance to stem rust in Medea durum wheat and the role of suppressors. Crop Sci 40:98–102CrossRefGoogle Scholar
  22. Kuraparthy V, Chhuneja P, Dhaliwal HS, Kaur S, Bowden RL, Gill BS (2007) Characterization and mapping of cryptic alien introgression from Aegilops geniculata with new leaf rust and stripe rust resistance genes Lr57 and Yr40 in wheat. Theor Appl Genet 114:1379–1389PubMedCrossRefGoogle Scholar
  23. Lin F, Chen XM (2007) Genetics and molecular mapping of genes for race-specific all-stage resistance and non-race-specific high-temperature adult-plant resistance to stripe rust in spring wheat cultivar Alpowa. Theor Appl Genet 114:1277–1287PubMedCrossRefGoogle Scholar
  24. Loegering WQ (1959) Methods for recording cereal rust data. USDA, International spring wheat rust nurseryGoogle Scholar
  25. Ma H, Singh RP, Mujeeb-Kazi A (1995) Suppression/expression of resistance to stripe rust in synthetic hexaploid wheat (T. turgidum X T. tauschii). Euphytica 83:87–93CrossRefGoogle Scholar
  26. Ma H, Singh RP, Mujeeb-Kazi A (1997) Resistance to stripe rust in durum wheats, A-genome diploids, and their amphiploids. Euphytica 94:279–286CrossRefGoogle Scholar
  27. Manly KF, Cudmore RH Jr, Meer JM (2001) Map Manager QTX, cross-platform software for genetic mapping. Mammal Genome 12:930–932CrossRefGoogle Scholar
  28. Mao Y, Xu S (2004) Mapping QTLs for traits measured as percentages. Genet Res 83:159–168PubMedCrossRefGoogle Scholar
  29. Marais GF, McCallum B, Marais AS (2006) Leaf rust and stripe rust resistance genes derived from Aegilops sharonensis. Euphytica 149:373–380CrossRefGoogle Scholar
  30. Marais GF, McCallum B, Snyman JE, Pretorius ZA, Marais AS (2005a) Leaf rust and stripe rust resistance genes Lr54 and Yr37 transferred to wheat from Aegilops kotschyi. Plant Breed 124:538–541CrossRefGoogle Scholar
  31. Marais GF, Pretorius ZA, Wellings CR, McCallum B, Marais AS (2005b) Leaf rust and stripe rust resistance genes transferred to common wheat from Triticum dicoccoides. Euphytica 143:115–123CrossRefGoogle Scholar
  32. McIntosh RA, Devos KM, Dubcovsky J, Rogers WJ, Morris CF, Appels R, Anderson OD (2005) Catalogue of gene symbols: 2005 supplement. In: KOMUGI–Integrated Wheat Science Database. (http://www.grs.nig.ac.jp/wheat/komugi)
  33. McIntosh RA, Dyck PL, The TT, Cusick JE, Milne DL (1984) Cytogenetical studies in wheat. XIII. Sr35—a third gene from Triticum monococcum for resistance to Puccinia graminis tritici. Z Pflanzenzücht 92:1–14Google Scholar
  34. McIntosh RA, Wellings CR, Park RF (1995) Wheat rusts: an atlas of resistance genes. CSIRO, East Melbourne, Victoria 3002, Australia, p 200Google Scholar
  35. Nayar SK, Prashar M, Bhardwaj SC (1997) Manual of current techniques in wheat rusts. Research Bull. No.2, Regional Station, Flowerdale, Shimla 171002, India, p 32Google Scholar
  36. Peng JH, Fahima T, Roder MS, Li YC, Dahan A, Grama A, Ronin YI, Korol AB, Nevo E (1999) Microsatellite tagging of the stripe-rust resistance gene YrH52 derived from wild emmer wheat, Triticum dicoccoides, and suggestive negative crossover interference on chromosome 1B. Theor Appl Genet 98:862–872CrossRefGoogle Scholar
  37. Peterson RF, Campbell AB, Hannah AE (1948) A diagrammatic scale for estimating rust intensity on leaves and stems of cereals. Can J Res 26:496–500Google Scholar
  38. Prashar M, Bhardwaj SC, Jain SK, Datta D (2007) Pathotypic evolution in Puccinia striiformis in India during 1995–2004. Austr J Agric Res 58:602–604CrossRefGoogle Scholar
  39. Prins R, Marais GF (1999) A genetic study of the gametocidal effect of the Lr19 translocation of common wheat. S Afr J Plant Soil 16:10–14Google Scholar
  40. Qiu YC, Zhou RH, Kong XY, Zhang SS, Jia JZ (2005) Microsatellite mapping of a Triticum urartu Tum. derived powdery mildew resistance gene transferred to common wheat (Triticum aestivum L.). Theor Appl Genet 111:1524–1531PubMedCrossRefGoogle Scholar
  41. Robert O, Abelard C, Dedryver F (1999) Identification of molecular markers for the detection of the yellow rust resistance gene Yr17 in wheat. Mol Breed 5:167–175CrossRefGoogle Scholar
  42. Roelfs AP, Singh RP, Saari EE (1992) Rust diseases of wheat: concept and methods of disease management. CIMMYT, MexicoGoogle Scholar
  43. Shi AN, Leath S, Murphy JP (1998) A Major gene for powdery mildew resistance transferred to common wheat from wild einkorn wheat. Phytopathology 88:144–147CrossRefPubMedGoogle Scholar
  44. Singh K, Chhuneja P, Ghai M, Kaur S, Goel RK, Bains NS, Keller B, Dhaliwal HS (2007a) Molecular mapping of leaf and stripe rust resistance genes in Triticum monococcum and their transfer to hexaploid wheat. In: Buck H, Nisi JE, Solomon N (eds) Wheat production in stressed environments. Springer, Netherlands, pp 779–786CrossRefGoogle Scholar
  45. Singh K, Ghai M, Garg M, Chhuneja P, Kaur P, Schnurbusch T, Keller B, Dhaliwal HS (2007b) An integrated molecular linkage map of diploid wheat based on a Triticum boeoticum X T. monococcum RIL population. Theor Appl Genet 115:301–312PubMedCrossRefGoogle Scholar
  46. Singh RP, Huerta-Espino J, William HM (2005) Genetics and breeding for durable resistance to leaf and stripe rusts in wheat. Turk J Agric For 29:121–127Google Scholar
  47. Singh RP, Nelson JC, Sorrells ME (2000) Mapping Yr28 and other genes for resistance to stripe rust in wheat. Crop Sci 40:1148–1155CrossRefGoogle Scholar
  48. Singh RP, William HM, Huerta-Espino J, Rosewarne G (2004) Wheat rust in Asia: meeting the challenges with old and new technologies. In: New directions for a diverse planet. Proceedings of the 4th international crop science congress, 26 September–1–October 2004, Brisbane, AustraliaGoogle Scholar
  49. Stakman EC, Stewart DH, Loegering WQ (1962) Identification of physiological pathotypes of Puccinia graminis var. tritici. USDA Agri Res Serv No. E617 (Rev), p 53Google Scholar
  50. The TT, Baker EP (1975) Basic studies relating to the transference of genetic characters from Triticum monococcum L. to hexaploid wheat. Aust J Biol Sci 28:189–199Google Scholar
  51. Uauy C, Brevis JC, Chen X, Khan I, Jackson L, Chicaiza O, Distelfeld A, Fahima T, Dubcovsky J (2005) High-temperature adult-plant (HTAP) stripe rust resistance gene Yr36 from Triticum turgidum ssp. dicoccoides is closely linked to the grain protein content locus Gpc-B1. Theor Appl Genet 112:97–105PubMedCrossRefGoogle Scholar
  52. Valkoun J, Kucerova D, Bartos P (1986) Transfer of leaf rust resistance from Triticum monococcum L. to hexaploid wheat. Z Pflanzenzucht 96:271–278Google Scholar
  53. Valkoun J, Kucerova D, Bartos P (1989) Transfer of a new gene for stem rust resistance from Triticum monoccocum L. to hexaploid wheat T. aestivum L. Genetika a Slechtini 25:209–212Google Scholar
  54. Villareal RL, Singh RP, Mujeeb-Kazi A (1992) Expression of resistance to Puccinia recondita f.sp. tritici in synthetic hexaploid wheats. Vortr Pflanzenzucht 24:253–255Google Scholar
  55. Worland AJ, Law CN (1986) Genetic analysis of chromosome 2D of wheat I. The location of genes affecting height, day-length insensitivity, hybrid dwarfism and yellow-rust resistance. Z Pflanzenzucht 96:331–345Google Scholar
  56. Yang J, Zhu J, Williams RW (2007) Mapping the genetic architecture of complex traits in experimental populations. Bioinformatics 23:1527–1536PubMedCrossRefGoogle Scholar
  57. Yang R, Yi N, Xu S (2006) Box-Cox transformation for QTL mapping. Genetica 128:133–143PubMedCrossRefGoogle Scholar
  58. Yao G, Zhang J, Yang L, Xu H, Jiang Y, Xiong L, Zhang C, Zhengzhi Z, Ma Z, Sorrells ME (2007) Genetic mapping of two powdery mildew resistance genes in einkorn (Triticum monococcum L.) accessions. Theor Appl Genet 114:351–358PubMedCrossRefGoogle Scholar
  59. Young ND, Tanksley SD (1989) RFLP analysis of the size of chromosomal segment retained around TM-2 locus of tomato during backcross breeding. Theor Appl Genet 77:353–359CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Parveen Chhuneja
    • 1
  • Satinder Kaur
    • 1
  • Tosh Garg
    • 1
  • Meenu Ghai
    • 1
  • Simarjit Kaur
    • 1
  • M. Prashar
    • 2
  • N. S. Bains
    • 1
  • R. K. Goel
    • 1
  • Beat Keller
    • 3
  • H. S. Dhaliwal
    • 1
    • 4
  • Kuldeep Singh
    • 1
  1. 1.Department of Plant Breeding, Genetics and BiotechnologyPunjab Agricultural UniversityLudhianaIndia
  2. 2.Directorate of Wheat Research, Regional StationShimlaIndia
  3. 3.Institute of Plant BiologyUniversity of ZurichZurichSwitzerland
  4. 4.Indian Institute of TechnologyRoorkeeIndia

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