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QTL mapping of yield, agronomic and quality traits in tetraploid potato (Solanum tuberosum subsp. tuberosum)

Abstract

Interval mapping of quantitative trait loci (QTL) for 16 yield, agronomic and quality traits in potato was performed on a tetraploid full-sib family comprising 227 clones from a cross between processing clone 12601ab1 and table cultivar Stirling. Thirty-eight AFLP primer combinations and six SSRs provided 514 informative markers which formed a molecular marker map comprising 12 linkage groups (LGs) in 12601ab1 (nine with four homologous chromosomes) which were aligned with 12 in Stirling (11 with four homologous chromosomes), with four partial groups remaining in 12601ab1. Two LGs were identified unequivocally as chromosomes IV and V and eight others were tentatively assigned with chromosomes VII and X unidentified. All of the traits scored had moderately high heritabilities with 54–92% of the variation in clone means over 3 years and two replicates being due to genetic differences. A total of 39 QTLs were identified. A QTL for maturity was identified on chromosome V which explained 56% of the phenotypic variance, whereas the other QTLs individually explained between 5.4 and 16.5%. However, six QTLs were detected for after-cooking blackening and four for each of regularity of tuber shape, fry colour both after storage at 4 and 10°C and sprouting. Just two QTLs were found for each of yield, the two ‘overall’ scores, crop emergence, tuber size and common scab and just one QTL was detected for each of dry matter content, keeping quality, growth cracks and internal condition. The implications for practical potato breeding and for practical linkage and QTL analysis in autotetraploids are discussed.

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Acknowledgements

Thanks are due to Margaret McInroy, Karen McLean, Drummond Todd and Ralph Wilson for technical support, Jim McNicol for statistical advice, and to the British Potato Council (Ph.D. studentship to B.P.), the Scottish Executive Environment and Rural Affairs Department and the United Kingdom Biotechnology and Biological Sciences Research Council (GAIT Project) for funding. Dr. Zewei Luo, now at the University of Birmingham, developed the theory and software for linkage analysis whilst on the GAIT project.

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Correspondence to John E. Bradshaw.

Additional information

Communicated by M. Kearsey.

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Bradshaw, J.E., Hackett, C.A., Pande, B. et al. QTL mapping of yield, agronomic and quality traits in tetraploid potato (Solanum tuberosum subsp. tuberosum). Theor Appl Genet 116, 193–211 (2008). https://doi.org/10.1007/s00122-007-0659-1

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Keywords

  • Quantitative Trait Locus
  • Late Blight
  • Homology Group
  • Homologous Chromosome
  • Common Scab