Theoretical and Applied Genetics

, Volume 116, Issue 1, pp 63–75 | Cite as

Trigenomic chromosomes by recombination of Thinopyrum intermedium and Th. ponticum translocations in wheat

  • L. Ayala-Navarrete
  • H. S. Bariana
  • R. P. Singh
  • J. M. Gibson
  • A. A. Mechanicos
  • P. J. Larkin
Original Paper

Abstract

Rusts and barley yellow dwarf virus (BYDV) are among the main diseases affecting wheat production world wide for which wild relatives have been the source of a number of translocations carrying resistance genes. Nevertheless, along with desirable traits, alien translocations often carry deleterious genes. We have generated recombinants in a bread wheat background between two alien translocations: TC5, ex-Thinopyrum (Th) intermedium, carrying BYDV resistance gene Bdv2; and T4m, ex-Th. ponticum, carrying rust resistance genes Lr19 and Sr25. Because both these translocations are on the wheat chromosome arm 7DL, homoeologous recombination was attempted in the double hemizygote (TC5/T4m) in a background homozygous for the ph1b mutation. The identification of recombinants was facilitated by the use of newly developed molecular markers for each of the alien genomes represented in the two translocations and by studying derived F2, F3 and doubled haploid populations. The occurrence of recombination was confirmed with molecular markers and bioassays on families of testcrosses between putative recombinants and bread wheat, and in F2 populations derived from the testcrosses. As a consequence it has been possible to derive a genetic map of markers and resistance genes on these previously fixed alien linkage blocks. We have obtained fertile progeny carrying new tri-genomic recombinant chromosomes. Furthermore we have demonstrated that some of the recombinants carried resistance genes Lr19 and Bdv2 yet lacked the self-elimination trait associated with shortened T4 segments. We have also shown that the recombinant translocations are fixed and stable once removed from the influence of the ph1b. The molecular markers developed in this study will facilitate selection of individuals carrying recombinant Th. intermediumTh. ponticum translocations (Pontin series) in breeding programs.

Keywords

Leaf Rust Rust Resistance Gene Translocation Line Barley Yellow Dwarf Virus Stem Rust Resistance Gene 

Notes

Acknowledgments

Thanks to Dr. Wolfgang Spielmeyer for his generous and effective advice during the research and the manuscript preparation, to Dr. Richard Richards for his ongoing enthusiasm and support for this work; to Dr. David Bonnett for making useful early crosses of Batavia 19-1-1 containing the Tm4 EMS mutated translocation; to the Wheat Genetics Resource Center, Kansas State University for providing the set of deletion lines for chromosome 7DL; to Dr. Ian Dundas, University of Adelaide for providing ph1b gene in Angas background. We acknowledge the generous financial support of the Grains Research and Development Corporation.

Supplementary material

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • L. Ayala-Navarrete
    • 1
  • H. S. Bariana
    • 2
  • R. P. Singh
    • 3
  • J. M. Gibson
    • 1
  • A. A. Mechanicos
    • 1
  • P. J. Larkin
    • 1
  1. 1.CSIRO Plant IndustryCanberraAustralia
  2. 2.Plant Breeding Institute CobbittyThe University of SydneyCamdenAustralia
  3. 3.International Maize and Wheat Improvement Center (CIMMYT)Mexico DFMexico

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