Theoretical and Applied Genetics

, Volume 116, Issue 1, pp 63–75 | Cite as

Trigenomic chromosomes by recombination of Thinopyrum intermedium and Th. ponticum translocations in wheat

  • L. Ayala-Navarrete
  • H. S. Bariana
  • R. P. Singh
  • J. M. Gibson
  • A. A. Mechanicos
  • P. J. Larkin
Original Paper


Rusts and barley yellow dwarf virus (BYDV) are among the main diseases affecting wheat production world wide for which wild relatives have been the source of a number of translocations carrying resistance genes. Nevertheless, along with desirable traits, alien translocations often carry deleterious genes. We have generated recombinants in a bread wheat background between two alien translocations: TC5, ex-Thinopyrum (Th) intermedium, carrying BYDV resistance gene Bdv2; and T4m, ex-Th. ponticum, carrying rust resistance genes Lr19 and Sr25. Because both these translocations are on the wheat chromosome arm 7DL, homoeologous recombination was attempted in the double hemizygote (TC5/T4m) in a background homozygous for the ph1b mutation. The identification of recombinants was facilitated by the use of newly developed molecular markers for each of the alien genomes represented in the two translocations and by studying derived F2, F3 and doubled haploid populations. The occurrence of recombination was confirmed with molecular markers and bioassays on families of testcrosses between putative recombinants and bread wheat, and in F2 populations derived from the testcrosses. As a consequence it has been possible to derive a genetic map of markers and resistance genes on these previously fixed alien linkage blocks. We have obtained fertile progeny carrying new tri-genomic recombinant chromosomes. Furthermore we have demonstrated that some of the recombinants carried resistance genes Lr19 and Bdv2 yet lacked the self-elimination trait associated with shortened T4 segments. We have also shown that the recombinant translocations are fixed and stable once removed from the influence of the ph1b. The molecular markers developed in this study will facilitate selection of individuals carrying recombinant Th. intermediumTh. ponticum translocations (Pontin series) in breeding programs.


Leaf Rust Rust Resistance Gene Translocation Line Barley Yellow Dwarf Virus Stem Rust Resistance Gene 



Thanks to Dr. Wolfgang Spielmeyer for his generous and effective advice during the research and the manuscript preparation, to Dr. Richard Richards for his ongoing enthusiasm and support for this work; to Dr. David Bonnett for making useful early crosses of Batavia 19-1-1 containing the Tm4 EMS mutated translocation; to the Wheat Genetics Resource Center, Kansas State University for providing the set of deletion lines for chromosome 7DL; to Dr. Ian Dundas, University of Adelaide for providing ph1b gene in Angas background. We acknowledge the generous financial support of the Grains Research and Development Corporation.

Supplementary material


  1. Akhunov ED, Goodyear AW, Geng S, Qi L-L, Echalier B, Gill BS, Miftahudin, Gustafson JP, Lazo G, Chao SM, Anderson OD, Linkiewicz AM, Dubcovsky J, La Rota M, Sorrells ME, Zhang DS, Nguyen HT, Kalavacharla V, Hossain K, Kianian SF, Peng JH, Lapitan NLV, Gonzalez-Hernandeiz JL, Anderson JA, Choi DW, Close TJ, Dilbirligi M, Gill KS, Walker-Simmons MK, Steber C, McGuire PE, Qualset CO, Dvorak J (2003) The organization and rate of evolution of wheat genomes are correlated with recombination rates along chromosome arms. Genome Res 13:753–763PubMedCrossRefGoogle Scholar
  2. Ayala L, Henry M, Gonzalez-de-Leon D, van Ginkel M, Mujeeb-Kazi A, Keller B, Khairallah M (2001) A diagnostic molecular marker allowing the study of Th. intermedium-derived resistance to BYDV in bread wheat segregating populations. Theor Appl Genet 102:942–949CrossRefGoogle Scholar
  3. Banks PM, Larkin PJ, Bariana HS, Lagudah ES, Appels R, Waterhouse PM, Brettell RIS, Chen X, Xu HJ, Xin ZY, Qian YT, Zhou XM, Cheng ZM, Zhou GH (1995) The use of cell culture for sub-chromosomal introgressions of barley yellow dwarf virus resistance from Thinopyrum intermedium to wheat. Genome 38:395–405PubMedGoogle Scholar
  4. Bariana HS, McIntosh RA (1993) Cytogenetic studies in wheat XIV. Location of rust resistance genes in VPM1 and their genetic linkage with other disease resistance genes in chromosome 2A. Genome 36:476–482PubMedGoogle Scholar
  5. Bhardwaj SC, Prashar M, Kumar S, Jain SK, Datta D (2005) Lr19 resistance in wheat becomes susceptible to Puccinia triticina in India. Plant Dis 89:1360–1360CrossRefGoogle Scholar
  6. Cai X, Jones S (1997) Direct evidence for high level of autosyndetic pairing in hybrids of Thinopyrum intermedium and Th. ponticum with Triticum aestivum. Theor Appl Genet 95:568–572CrossRefGoogle Scholar
  7. Chen Q, Conner RL, Laroche A, Thomas JB (1998) Genome analysis of Thinopyrum intermedium and Thinopyrum ponticum using genomic in situ hybridization. Genome 41:580–586PubMedCrossRefGoogle Scholar
  8. Crasta OR, Francki MG, Bucholtz DB, Sharma HC, Zhang J, Wang RC, Ohm HW, Anderson JM (2000) Identification and characterization of wheat–wheatgrass translocation lines and localization of barley yellow dwarf virus resistance. Genome 43:698–706PubMedCrossRefGoogle Scholar
  9. Dvorak J (1981) Nonstructural chromosome differentiation among wheat cultivars, with special reference to differentiation of chromosomes in related species. Genetics 97:391PubMedGoogle Scholar
  10. Dvorak J, Knott DR (1977) Homoeologous chromatin exchange in a radiation-induced gene transfer. Can J Genet Cytol 19:125–131Google Scholar
  11. Elyasi-Gomari S, Panteleev VK (2006) Virulence polymorphism of Puccinia recondita f. sp tritici and effectiveness of Lr genes for leaf rust resistance of wheat in Ukraine. Plant Dis 90:853–857CrossRefGoogle Scholar
  12. Endo TR, Gill BS (1996) The deletion stocks of common wheat. J Hered 87:295–307Google Scholar
  13. Erayman M, Sandhu D, Sidhu D, Dilbirligi M, Baenziger PS, Gill KS (2004) Demarcating the gene-rich regions of the wheat genome. Nucleic Acids Res 32:3546–3565PubMedCrossRefGoogle Scholar
  14. Fedak G, Han F (2005) Characterization of derivatives from wheat–Thinopyrum wide crosses. Cytogenet Genome Res 109:360–367PubMedCrossRefGoogle Scholar
  15. Fedak G, Chen Q, Conner RL, Laroche A, Comeau A, St-Pierre CA (2001) Characterization of wheat–Thinopyrum partial amphiploids for resistance to barley yellow dwarf virus. Euphytica 120:373–378CrossRefGoogle Scholar
  16. Feuillet C, Keller B (2002) Comparative genomics in the grass family: molecular characterization of grass genome structure and evolution. Ann Bot 89:3–10PubMedCrossRefGoogle Scholar
  17. Francki MG, Ohm HW, Anderson JM (2001) Novel germplasm providing resistance to barley yellow dwarf virus in wheat. Aust J Agric Res 52:1375–1382CrossRefGoogle Scholar
  18. Groenewald JZ, Fourie M, Marais AS, Marais GF (2005) Extension and use of a physical map of the Thinopyrum-derived Lr19 translocation. Theor Appl Genet 112:131–138PubMedCrossRefGoogle Scholar
  19. Gupta SK, Charpe A, Prabhu KV, Haque QMR (2006) Identification and validation of molecular markers linked to the leaf rust resistance gene Lr19 in wheat. Theor Appl Genet 113:1027–1036PubMedCrossRefGoogle Scholar
  20. Han FP, Liu B, Fedak G, Liu ZH (2004) Genomic constitution and variation in five partial amphiploids of wheat–Thinopyrum intermedium as revealed by GISH, multicolor GISH and seed storage protein analysis. Theor Appl Genet 109:1070–1076PubMedCrossRefGoogle Scholar
  21. Hossain KG, Lazo GR, Hegstad J, Wentz MJ, Kianian PMA, Simons K, Gehlhar S, Rust JL, Syamala RR, Obeori K, Bhamidimarri S, Karunadharma P, Chao S, Anderson OD, Qi LL, Echalier B, Gill BS, Linkiewicz AM, Ratnasiri A, Dubcovsky J, Akhunov ED, Dvorak J, Miftahudin, Ross K, Gustafson JP, Radhawa HS, Dilbirligi M, Gill KS, Peng JH, Lapitan NLV, Greene RA, Bermudez-Kandianis CE, Sorrells ME, Feril O, Pathan MS, Nguyen HT, Gonzalez-Hernandez JL, Conley EJ, Anderson JA, Choi DW, Fenton D, Close TJ, McGuire PE, Qualset CO, Kianian SF (2004) A chromosome bin map of 2148 expressed sequence tag loci of wheat homoeologous group 7. Genetics 168:687–699PubMedCrossRefGoogle Scholar
  22. Huerta-Espino J, Singh RP (1994) First report of virulence to wheat with leaf rust resistance gene Lr19 in Mexico. Plant Dis 78:640CrossRefGoogle Scholar
  23. Jauhar PP (1995) Meiosis and fertility of F1 hybrids between hexaploid bread wheat and decaploid tall wheatgrass (Thinopyrum-ponticum) Theor Appl Genet 90:865–871Google Scholar
  24. Jubault M, Tangyu AM, Abelard P, Coriton O, Dusautoir JC, Jahier J (2006) Attempts to induce homoeologous pairing between wheat and Agropyron cristatum genomes. Genome 49:190–193PubMedGoogle Scholar
  25. Khan IA (2000) Molecular and agronomic characterization of wheat–Agropyron intermedium recombinant chromosomes. Plant Breed 119:25–29CrossRefGoogle Scholar
  26. Kishii M, Wang RRC, Tsujimoto H (2005) GISH analysis revealed new aspect of genomic constitution of Thinopyrum intermedium. In: 5th international Triticeae symposium, Book of abstracts. Prague, Czech Republic, p 21Google Scholar
  27. Knott DR (1980) Mutation of a gene for yellow pigment linked to Lr19 in wheat. Can J Genet Cytol 22:651–654Google Scholar
  28. Larkin PJ, Banks PM, Lagudah ES, Appels R, Xiao C, Xin Z, Ohm HW, McIntosh RA (1995) Disomic alien addition lines in wheat representing chromosomes from Thinopyrum intermedium with barley yellow dwarf virus (BYDV) resistance and with rust resistance. Genome 38:385–394PubMedGoogle Scholar
  29. Larkin PJ, Kleven S, Banks PM (2002) Developing wheat cultivars utilizing Bdv2, the Thinopyrum intermedium source of barley yellow dwarf virus resistance. In: Henry M, McNab A (eds) Barley yellow dwarf disease: recent advances and future strategies. CIMMYT, Mexico, pp 60–63Google Scholar
  30. Liu ZW, Wang RRC (1993) Genome analysis of Elytrigia-caespitosa, Lophopyrum-nodosum, Pseudoroegneria-geniculata spp scythica, and Thinopyrum intermedium (Triticeae, Gramineae). Genome 36:102–111PubMedGoogle Scholar
  31. Lukaszewski AJ (2000) Manipulation of the 1RS.1BL translocation in wheat by induced homoeologous recombination. Crop Sci 40:216–225CrossRefGoogle Scholar
  32. Lukaszewski AJ (2003) Registration of six germplasms of bread wheat having variations of cytogenetically engineered wheat–rye translocation 1RS.1BL. Crop Sci 43:1137–1138CrossRefGoogle Scholar
  33. Lyubimova VF (1970) Cytogenetic investigations of hybrids obtained from crossing Agropyron glaucum Roem. Et Schult with Agropyron elongatum (Host) PB. Genetika 6:5–15. Transl Sov Genet 6:1135–1143Google Scholar
  34. Marais GF (1991) Gamma irradiation induced deletions in an alien chromosome segment of the wheat ‘Indis’ and their use in gene mapping. Genome 35:225–229Google Scholar
  35. Marais GF (1992) The modification of a common wheat–Thinopyrum-distichum translocated chromosome with a locus homeoallelic to Lr19. Theor Appl Genet 85:73–78CrossRefGoogle Scholar
  36. Marais GF, Marais AS, Groenewald JZ (2001) Evaluation and reduction of Lr19-149, a recombined from of the Lr19 translocation of wheat. Euphytica 121:289–295CrossRefGoogle Scholar
  37. McCallum BD, Seto-Goh P (2006) Physiologic specialization of Puccinia triticina, the causal agent of wheat leaf rust, in Canada in 2003. Can J Plant Pathol 28:208–213CrossRefGoogle Scholar
  38. McIntosh RA, Wellings CR, Park RF (1995) Wheat rusts: an atlas of resistance genes. CSIRO Publications, East Melbourne, p 200Google Scholar
  39. Molnar-Lang M, Novotny C, Linc G, Nagy ED (2005) Changes in the meiotic pairing behaviour of a winter wheat–winter barley hybrid maintained for a long term in tissue culture, and tracing the barley chromatin in the progeny using GISH and SSR markers. Plant Breed 124:247–252CrossRefGoogle Scholar
  40. Monneveux P, Reynolds MP, González Aguilar J, Singh RP (2003) Effects of the 7DL.7Ag translocation from Lophopyrum elongatum on wheat yield and related morphophysiological traits under different environments. Plant Breed 122:379–384CrossRefGoogle Scholar
  41. Moor G, Devos KM, Wang Z, Gale MD (1995) Grasses, line up and form a circle. Curr Biol 5:737–739CrossRefGoogle Scholar
  42. Ohm HW, Anderson JM, Sharma HC, Ayala L, Thompson N, Uphaus JJ (2005) Registration of yellow dwarf viruses resistant wheat germplasm line P961341. Crop Sci 45:805–806CrossRefGoogle Scholar
  43. Pestova E, Salina E, Borner A, Korzun V, Maystrenki OI, Roder MS (2000) Microsatellites confirm the authenticity of inter-varietal chromosome substitution lines of what (Triticum aestivum L.). Theor Appl Genet 101:95–99CrossRefGoogle Scholar
  44. Prins R, Marais GF (1998) An extended deletion map of the Lr19 translocation and modified forms. Euphytica 103:95–102CrossRefGoogle Scholar
  45. Prins R, Marais GF, Janse BJH, Pretorius ZA, Marais AS (1996) A physical map of the Thinopyrum-derived Lr19 translocation. Genome 39:1013–1019PubMedGoogle Scholar
  46. Prins R, Marais GF, Pretorius ZA, Janse BJH, Marais AS (1997) A study of modified forms of the Lr19 translocation of common wheat. Theor Appl Genet 95:424–430CrossRefGoogle Scholar
  47. Prins R, Groenewald JZ, Marais GF, Snape JW (2001) AFLP and STS tagging of Lr19, a gene conferring resistance to leaf rust in wheat. Theor Appl Genet 103:618–624CrossRefGoogle Scholar
  48. Roder MS, Korzun V, Wendehake K, Plaschke J, Tixier M-H, Leroy P, Ganal M (1998) A microsatellite map of wheat. Genetics 149:2007–2023PubMedGoogle Scholar
  49. Sandhu D, Gill KS (2002) Gene-containing regions of wheat and other grass genomes. Plant Physiol 128:803–811PubMedCrossRefGoogle Scholar
  50. Sharma D, Knott DR (1966) The transfer of leaf rust resistance from Agropyron to Triticum by irradiation. Can J Genet Cytol 8:137–143Google Scholar
  51. Sharma H, Ohm H, Goulart L, Lister R, Applels R, Benlhabib O (1995) Introgression and characterization of barley yellow dwarf virus resistance from Thinopyrum intermedium into wheat. Genome 38:406–413PubMedGoogle Scholar
  52. Sibikeeva YE, Sibikeev SN, Krupnov VA (2004) The effect of Lr19-translocation on in vitro androgenesis and inheritance of leaf-rust resistance in DH3 lines and F2 hybrids of common wheat. Russ J Genet 40:1003–1006CrossRefGoogle Scholar
  53. Singh RP, Rajaram S (1991) Resistance to Puccinia recondita f.sp tritici in 50 Mexican bread wheat cultivars. Crop Sci 31:1472–1479CrossRefGoogle Scholar
  54. Singh RP, Burnett PA, Albarran M, Rajaram S (1993) Bdv1: a gene for tolerance to barley yellow dwarf virus in bread wheats. Crop Sci 33:231–234CrossRefGoogle Scholar
  55. Singh RP, Huerta-Espino J, Rajaram S, Crossa J (1998) Agronomic effects from chromosome translocations 7DL.7Ag and 1BL.1RS in spring wheat. Crop Sci 38:27–33CrossRefGoogle Scholar
  56. Sorrells ME, La Rota M, Bermudez-Kandianis CE, Greene RA, Kantety R et al (2003) Comparative DNA sequence analysis of wheat and rice genomes. Genome Res 13:1818–1827PubMedGoogle Scholar
  57. Stoutjesdijk P, Kammholz SJ, Kleven S, Matsay S, Banks PM, Larkin PJ (2001) PCR-based molecular marker for the Bdv2 Thinopyrum intermedium source of barley yellow dwarf virus resistance in wheat. Aust J Agric Res 52:1383–1388CrossRefGoogle Scholar
  58. Wang RR-C, Wei J-Z (1995) Variations of two repetitive DNA sequences in several Triticeae genomes revealed by polymerase chain reaction and sequencing. Genome 38:1221–1229PubMedCrossRefGoogle Scholar
  59. Wang RR-C, Zhang XY (1996) Characterization of the translocated chromosome using fluorescence in situ hybridization and random amplified polymorphic DNA on two Triticum aestivumThinopyrum intermedium translocation lines resistant to wheat streak mosaic or barley yellow dwarf virus. Chromosome Res 4:583–587PubMedCrossRefGoogle Scholar
  60. Wang RR-C, von Bothmer R, Dvorak J, Fedak G, Linde-Lauysen I, Muramatsu M (1994) Genomic symbols in Triticeae. In: Wang RR-C, Jensen KB, Jaussi C (eds) Proceedings of the 2nd international Triticeae symposium. Logan, Utah, 20–24 June 1994. Utah State University, Logan, pp 29–34Google Scholar
  61. Wang XW, Lai JR, Liu GT, Chen F (2002) Development of a SCAR marker for the Ph1 locus in common wheat and its application. Crop Sci 42:1365–1368CrossRefGoogle Scholar
  62. Xin ZY, Zhang ZY, Chen X, Lin ZS, Ma YZ, Xu HJ, Banks PM, Larkin PJ (2001) Development and characterization of common wheat–Thinopyrum intermedium translocation lines with resistance to barley yellow dwarf virus. Euphytica 119:161–165CrossRefGoogle Scholar
  63. Zhang XY (1992) Cytogenetic research on hybrids of Triticum with both Thinopyrum ponticum (2n = 70) and Th. intermedium (2n = 42) as well as their derivatives, Ph.D. dissertation. Graduate School of Chinese Academy of Agricultural SciencesGoogle Scholar
  64. Zhang HB, Dvorak J (1990) Isolation of repeated DNA-sequences from Lophopyrum-elongatum for detection of Lophopyrum chromatin in wheat genomes. Genome 33:283–293Google Scholar
  65. Zhang XY, Koul A, Petroski R, Ouellet T, Fedak G, Dong YS, Wang RRC (1996) Molecular verification and characterization of BYDV-resistant germplasms derived from hybrids of wheat with Thinopyrum ponticum and Th. intermedium. Theor Appl Genet 93:1033–1039CrossRefGoogle Scholar
  66. Zhang W, Lukaszewski JA, Kolmer J, Soria MA, Goyal S, Dubcovsky J (2005) Molecular characterization of durum and common wheat recombinant lines carrying leaf rust resistance (Lr19) and yellow pigment (Y) genes from Lophopyrum ponticum. Theor Appl Genet 111:573–582PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • L. Ayala-Navarrete
    • 1
  • H. S. Bariana
    • 2
  • R. P. Singh
    • 3
  • J. M. Gibson
    • 1
  • A. A. Mechanicos
    • 1
  • P. J. Larkin
    • 1
  1. 1.CSIRO Plant IndustryCanberraAustralia
  2. 2.Plant Breeding Institute CobbittyThe University of SydneyCamdenAustralia
  3. 3.International Maize and Wheat Improvement Center (CIMMYT)Mexico DFMexico

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