Theoretical and Applied Genetics

, Volume 115, Issue 8, pp 1137–1145 | Cite as

Identification of a novel mitochondrial genome type and development of molecular markers for cytoplasm classification in radish (Raphanus sativus L.)

  • Sunggil Kim
  • Heerae Lim
  • Suhyung Park
  • Kang-Hee Cho
  • Soon-Kee Sung
  • Dae-Geun Oh
  • Ki-Taek KimEmail author
Original Paper


Plant mitochondrial genomes have complex configurations resulting from the multipartite structures and highly rearranged substoichiometric molecules created by repetitive sequences. To expedite the reliable classification of the diverse radish (Raphanus sativus L.) cytoplasmic types, we have developed consistent molecular markers within their complex mitochondrial genomes. orf138, a gene responsible for Ogura male-sterility, was detected in normal cultivars in the form of low-copy-number substoichiometric molecules. In addition to the dominant orf138-atp8 Ogura mitochondrial DNA (mtDNA) organization, three novel substoichiometric organizations linked to the atp8 gene were identified in this study. PCR amplification profiles of seven atp8- and atp6-linked sequences were divided into three groups. Interestingly, the normal cytoplasm type, which had previously been considered a single group, showed two patterns by PCR amplification. The most prominent difference between the two normal mtDNAs was size variation within four short-repeat sequences linked to the atp6 gene. This variation appeared to be the result of a double crossover, mediated by these homologous, short-repeat sequences. Specific PCR amplification profiles reflecting the stoichiometry of different mtDNA fragments were conserved within cultivars and across generations. Therefore, the specific sequences detected in these profiles were used as molecular markers for the classification of diverse radish germplasm. Using this classification system, a total of 90 radish cultivars, or accessions, were successfully assigned to three different mitotypes.


Mitochondrial Genome Atp8 Gene Orf138 Gene Plant Mitochondrial Genome Normal Radish 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by a grant (Code#20050401034824) from BioGreen 21 Program, Rural Development Administration, Republic of Korea.

Supplementary material

122_2007_639_MOESM1_ESM.doc (78 kb)
(DOC 77 kb)


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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Sunggil Kim
    • 1
    • 3
  • Heerae Lim
    • 1
  • Suhyung Park
    • 2
  • Kang-Hee Cho
    • 2
  • Soon-Kee Sung
    • 1
  • Dae-Geun Oh
    • 2
  • Ki-Taek Kim
    • 2
    Email author
  1. 1.Biotech Research CenterDongbu Advanced Research InstituteDaejeonSouth Korea
  2. 2.National Horticultural Research InstituteRDASuwonSouth Korea
  3. 3.Department of Plant BiotechnologyChonnam National UniversityGwangjuSouth Korea

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