Theoretical and Applied Genetics

, Volume 115, Issue 7, pp 971–980 | Cite as

Development and validation of a Viviparous-1 STS marker for pre-harvest sprouting tolerance in Chinese wheats

Original Paper

Abstract

Pre-harvest sprouting (PHS) of wheat reduces the quality of wheat grain, and improving PHS tolerance is a priority in certain wheat growing regions where conditions favorable for PHS exist. Two new Viviparous-1 allelic variants related to PHS tolerance were investigated on B genome of bread wheat, and designated as Vp-1Bb and Vp-1Bc, respectively. Sequence analysis showed that Vp-1Bb and Vp-1Bc had an insertion of 193-bp and a deletion of 83-bp fragment, respectively, located in the third intron region of the Vp-1B gene. The insertion and deletion affected the expression level of the Vp1 at mature seed stage, more correctly spliced transcripts were observed from the genotypes with either insertion or deletion than that of the wild type. Based on these insertions and deletions, a co-dominant STS marker of Vp-1B gene was developed and designated as Vp1B3, which in most cases could amplify either 845 or 569-bp fragment from the tolerant cultivars, and 652-bp from the susceptible ones. This Vp1B3 marker was mapped to chromosome 3BL using a set of Chinese Spring nulli-tetrasomic and ditelosomic lines. A total of 89 white-grained Chinese wheat cultivars and advanced lines, were used to validate the relationship between the polymorphic fragments of Vp1B3 and PHS tolerance. Statistical analysis indicated that Vp1B3 was strongly associated with PHS tolerance in this set of Chinese germplasm, suggesting that Vp1B3 could be used as an efficient and reliable co-dominant marker in the evaluation of wheat germplasm for PHS tolerance and marker-assisted breeding for PHS tolerant cultivars.

References

  1. Alexei F, Scott R, Larisa F, Walter G (2003) Mystery of intron gain. Genome Res 13:2236–2241CrossRefGoogle Scholar
  2. Atsushi T, Yoichi A (2002) Effect of seed coat color on seed dormancy in different environments. Euphytica 126:99–105CrossRefGoogle Scholar
  3. Bailey PC, McKibbin RS, Lenton JR (1999) Genetic map location for orthologous VP1 genes in wheat and rice. Theor Appl Genet 98:281–284CrossRefGoogle Scholar
  4. Chang S, Puryear J, Cairney J (1993) A simple and efficient method for isolating RNA from pine trees. Plant Mol Biol Rep 11:113–116CrossRefGoogle Scholar
  5. Derera NF (1990) A perspective of sprouting research. In: Ringlud K, Mosleth E, Mares DJ (eds) Fifth international symposium on pre-harvest sprouting in cereal, pp 1–11Google Scholar
  6. Flintham JE (1993) Grain color and sprout resistance in wheat. In: Derera NF (ed) Pre-harvest sprouting in cereals 1992, pp 111–128Google Scholar
  7. Flintham JE (2000) Different genetic components control coat-imposed and embryo-imposed dormancy in wheat. Seed Sci Res 10:43–50Google Scholar
  8. Flintham JE, Adlam R, Gale M (1999) Seed coat and embryo dormancy in wheat. In: Weipert D (ed) 8th international symposium on pre-harvest sprouting in cereals 1998, pp 67–76Google Scholar
  9. Gale MD, Lenton JR (1987) Preharvest sprouting in wheat: a complex genetic and physiological problem affecting breadmaking quality in UK wheat. Asp Appl Biol 15:115–124Google Scholar
  10. Gale KR, Ma W, Zhang W, Rampling L, Hill AS, Appels R, Morris P, Morrel M (2001) Simple high-throughput DNA markers for genotyping in wheat. In: Eastwood R et al (ed) 10th Australian wheat breeding assembly proceedings, pp 26–31Google Scholar
  11. Giraudat J, Hauge BM, Valon C, Smalle J, Parcy F, Goodman HM (1992) Isolation of the Arabidopsis ABI3 gene by positional cloning. Plant Cell 4:1251–1261PubMedCrossRefGoogle Scholar
  12. Groos C, Gay G, Perretant MR, Gervais L, Bernard M, Dedryver F, Charmet G (2002) Study of the relationship between pre-harvest sprouting and grain color by quantitative trait loci analysis in a white × red grain bread-wheat cross. Theor Appl Genet 104:39–47PubMedCrossRefGoogle Scholar
  13. Himi E, Noda K (2005) Red grain color gene (R) of wheat is a Myb-type transcription factor. Euphytica 143:239–242CrossRefGoogle Scholar
  14. Himi E, Mares DJ, Yanagisawa A, Noda K (2002) Effect of grain color gene (R) on grain dormancy and sensitivity of the embryo to abscisic acid (ABA) in wheat. J Exp Bot 53:1569–1574PubMedCrossRefGoogle Scholar
  15. Humphreys DG, Noll J (2002) Methods for characterization of preharvest sprouting tolerance in a wheat breeding program. Euphytica 126:61–65CrossRefGoogle Scholar
  16. Jones HD, Peters NC, Holdsworth MJ (1997) Genotype and environment interact to control dormancy and differential expression of the VIVIPAROUS 1 homologue in embryos of Avena fatua. Plant J 12:911–20PubMedCrossRefGoogle Scholar
  17. Kato K, Nakamura W, Tabiki T, Miura H, Sawada S (2001) Detection of loci controlling seed dormancy in group 4 chromosomes of wheat and comparative mapping with rice and barley genomes. Theor Appl Genet 102:980–985CrossRefGoogle Scholar
  18. Li CD, Ni PX, Francki M, Hunter A, Zhang Y, Schibed D, Li H, Tarr A, Wang J, Cakir M, Yu J, Bellgard M, Lance R, Appels R (2004) Genes controlling seed dormancy and pre-harvest sprouting in a rice–wheat–barley comparison. Funct Integr Genomics 4:84–93PubMedCrossRefGoogle Scholar
  19. Lohwasser U, Roder MS, Borner A (2005) QTL mapping of the domestication traits pre-harvest sprouting and dormancy in wheat (Triticum aestivum L.). Euphytica 143:247–249CrossRefGoogle Scholar
  20. Mares D, Mrva K, Cheong J, Williams K, Watson B, Storlie E, Sutherland M, Zou Y (2005) A QTL located on chromosome 4A associated with dormancy in white- and red-grained wheats of diverse origin. Theor Appl Genet 111:1357–1364PubMedCrossRefGoogle Scholar
  21. McCarty DR, Hattori T, Carson CB, Vasil V, Lazar M, Vasil IK (1991) The Viviparous-1 developmental gene of maize encodes a novel transcriptional activator. Cell 66:895–905PubMedCrossRefGoogle Scholar
  22. McKibbin RS, Bailey PC, Flintham JE, Gale MD, Lenton JR, Holdsworth MJ (1999) Molecular analysis of the wheat viviparous 1 (VP1) orthologue. In: Weipert D (ed) Eighth international symposium on pre-harvest sprouting in cereals 1998. Association of Cereal Research. Detmold pp 113–118Google Scholar
  23. McKibbin RS, Wilkinson MD, Bailey PC, Flintham JE, Andrew LM, Lazzeri PA, Gale MD, Lenton JR, Holdworth MJ (2002) Transcripts of Vp-1 homologues are misspliced in modern wheat and ancestral species. Proc Natl Acad Sci USA 99:10203–10208PubMedCrossRefGoogle Scholar
  24. Nakamura S, Toyama T (2001) Isolation of a VP1 homologue from wheat and analysis of its expression in embryos of dormant and non-dormant cultivars. J Exp Bot 52:875–876PubMedCrossRefGoogle Scholar
  25. Roy JK, Prasad M, Varshney RK (1999) Identification of a microsatellite on chromosomes 6B and a STS on 7D of bread wheat showing an association with pre-harvest sprouting tolerance. Theor Appl Genet 99:336–340CrossRefGoogle Scholar
  26. Tan MK, Sharp PJ, Lu MQ, Hows N (2006) Genetics of grain dormancy in a white wheat. Aust J Agric Res 57:1157–1165CrossRefGoogle Scholar
  27. Walker-Simmons MK (1988) Enhancement of ABA responsiveness in wheat embryos at higher temperature. Plant Cell Environ 11:769-775CrossRefGoogle Scholar
  28. Warner R, Kudrna D, Spaeth S, Jones S (2000) Dormancy in wheat grain-mutant of Chinese Spring wheat (Triticum aestivum L.). Seed Sci Res 10:51–60Google Scholar
  29. Wilkinson M, Lenton J, Holdsworth M (2005) Transcripts of VP-1 homologues are alternatively spliced within the Triticeae tribe. Euphytica 143:243–246CrossRefGoogle Scholar
  30. Xiao SH, Dai DQ, Jiang HR (1986) Researches on pre-harvest sprouting tolerance wheat cultivars. J Sichuan Agric Univ 4:219–224Google Scholar
  31. Xiao SH, Yan CS, Zhang HP, Sun GZ (2004) Studies for preharvest sprouting of wheat. China Press of Agricultural Science and Technology. pp 266–292Google Scholar
  32. Yan L, Bhave M, Fairclough R, Konik C, Rahman S, Appels R (2000) The gene encoding granule-bound starch syntheses at the waxy loci of the A, B, and D progenitors of common wheat. Genome 43:264–272PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  1. 1.Institute of Crop Science, National Wheat Improvement Center, The National Key Facility for Crop Gene Resources and Genetic ImprovementChinese Academy of Agricultural Sciences (CAAS)BeijingChina
  2. 2.College of AgronomyInner Mongolia Agricultural UniversityHohhotChina
  3. 3.Wheat Research InstituteHenan Academy of Agricultural SciencesZhengzhouChina
  4. 4.CIMMYT China Office, C/O CAASBeijingChina
  5. 5.Leibiniz Institute of Plant Genetics and Crop Plant Research (IPK)GaterslebenGermany

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