Theoretical and Applied Genetics

, Volume 115, Issue 6, pp 859–867 | Cite as

Analyses of a multi-parent population derived from two diverse alfalfa germplasms: testcross evaluations and phenotype–DNA associations

  • I. J. Maureira-Butler
  • J. A. Udall
  • T. C. Osborn
Original Paper

Abstract

In a previous study, we showed that the genetic variation present in the Medicago sativa subsp. sativa Peruvian and M. sativa subsp. falcata WISFAL germplasms could be used to improve forage yields when favorable alleles were recombined and used in hybrid combination with cultivated alfalfa. In this paper, we present testcross forage yield and fall growth data for two seasons of a C0 population generated after intermating the Peruvian × WISFAL population for several generations. In addition, we conducted marker-trait association analysis as an attempt to identify Peruvian and WISFAL genomics regions affecting the targeted traits. Five and seven genomic regions were found significantly associated with forage yield and fall growth, respectively. In the case of fall growth, alleles from both accessions were positively associated with plant height. However, more alleles from WISFAL were positively associated with forage yield than from Peruvian. WISFAL is known for its winter hardiness and genomic regions with large effects on winter survival may have masked the effect of forage yield from Peruvian. The fact that most of the genomic regions discovered in this study have been previously associated with traits involved in winter hardiness validates our findings and suggests that associations between DNA fragments and agronomic traits can be detected without the necessity of developing bi-parental mapping populations.

References

  1. Barnes DK, Hanson CH (1971) Recurrent selection for bacterial wilt resistance in Alfalfa. Crop Sci 11:545–546CrossRefGoogle Scholar
  2. Barnes DK, Martin NP (1991) Varietal trials of selected farm crops. Minnesota Agric Exp Stn Rep 221-1991Google Scholar
  3. Bingham ET (1993) Registration of WISFAL alfalfa (Medicago sativa subsp falcata) tetraploid germplasm derived from diploids. Crop Sci 33:217–218CrossRefGoogle Scholar
  4. Bingham ET, Groose RW, Woodfield DR, Kidwell KK (1994) Complementary gene interactions in alfafa are greater in autotetraploids than diploids. Crop Sci 34:823–829CrossRefGoogle Scholar
  5. Bray RA, Irwin JAG (1989) Recurrent selection for resistance to Stemphylium versicarium within the lucerne cultivars Trifecta and Sequel. Austr J Exp Agr 29:189–192CrossRefGoogle Scholar
  6. Brouwer DJ, Osborn TC (1999) A molecular marker linkage map of tetraploid alfalfa (Medicago sativa L.). Theor Appl Genet 99:1194–1200CrossRefGoogle Scholar
  7. Brouwer DJ, Duke SH, Osborn TC (1998) Comparison of seedlings and cuttings for evaluating winter hardiness in alfalfa. Crop Sci 38:1704–1707CrossRefGoogle Scholar
  8. Brouwer DJ, Duke SH, Osborn TC (2000) Mapping genetic factors associated with winter hardiness, fall growth, and freezing injury in tetraploid alfalfa. Crop Sci 40:1387–1396CrossRefGoogle Scholar
  9. Brummer EC (1999) Capturing heterosis in forage crop cultivar development. Crop Sci 39:943–954CrossRefGoogle Scholar
  10. Brummer EC, Shah MM, Luth D (2000) Reexamining the relationship between fall dormancy and winter hardiness. Crop Sci 40:971–977CrossRefGoogle Scholar
  11. Busbice TH (1969) Inbreeding in synthetic varieties. Crop Sci 10:265–269CrossRefGoogle Scholar
  12. Busbice TH, Wilsie CP (1965) Fall growth, winter hardiness, recovery after cutting, and witl resistance in F2 progenies of Vernal × Dupuits alfalfa crosses. Crop Sci 5:429–432CrossRefGoogle Scholar
  13. Cao D, Craig BA, Doerge RW (2005) A model selection-based interval-mapping method for autotetraploids. Genetics 169:2371–2382PubMedCrossRefGoogle Scholar
  14. Champoux MC, Wang G, Sarkarung S, Mackill DJ, O’Toole JC, Huang N, McCouch SR (1995) Locating genes associated with root morphology and drought avoidance in rice via linkage to molecular markers. Theor Appl Genet 90:969–981CrossRefGoogle Scholar
  15. Churchill GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971PubMedGoogle Scholar
  16. Daday H, Greenham CC (1960) Genetic studies on cold hardiness in Medicago sativa L. J Hered 51:249–255Google Scholar
  17. Demment MW, Teuber LR, Bourque DP, Phillips DA (1986) Changes in forage quality of improved alfalfa populations. Crop Sci 26:1137–1143CrossRefGoogle Scholar
  18. Diwan N, Bouton JH, Kochert G, Creagan PB (2000) Mapping of simple sequence repeat (SSR) DNA markers in diploid and tetraploid alfalfa. Theor Appl Genet 101:165–172CrossRefGoogle Scholar
  19. Dobrenz AK, Smith SE, Poteet D, Miller WD (1993) Carbohydrates in alfalfa seed developed for salt tolerance during germination. Agron J 85:834–836CrossRefGoogle Scholar
  20. Doerge RW, Churchill GA (1996) Permutation tests for multiple loci affecting a quantitative character. Genetics 142:285–294PubMedGoogle Scholar
  21. Dudley JW, Busbice TH, Levings CS (1969) Estimates of genetic variance in Cherokee alfalfa (Medicago sativa L.). Crop Sci 9:228–231CrossRefGoogle Scholar
  22. Duvick DN (1984) Genetic contribution to yield gains of U.S. hybrid maize, 1930 to 1980. In: Fehr WR (eds) Genetic contributions to yield gains of five major crop plants.Special Publication Number 7. CSSA, Madison, pp 15–47Google Scholar
  23. Dunbier MW, Bingham ET (1975) Maximun heterozygosity in alfalfa: results using haploid-derived autotetraploids. Crop Sci 15:527–531CrossRefGoogle Scholar
  24. Echt CS, Kidwell KK, Knapp SJ, Osborn TC, McCoy TJ (1994) Linkage mapping in diploid alfalfa (Medicago sativa). Genome 37:61–71PubMedGoogle Scholar
  25. Elden TC, Elgin JH (1987) Recurrent seedling and individual plant selection for potato leafhopper (Homoptera: Cicadellidae) resistance in alfalfa. J Econ Entomol 80:690–695Google Scholar
  26. Groose RW, Talbert LE, Kojis WP, Bingham ET (1989) Progressive heterosis in autotetraploid alfalfa: studies using two types of inbreeds. Crop Sci 29:1173–1177CrossRefGoogle Scholar
  27. Heisey RF, Murphy RP (1985) Phenotypic recurrent selection for resistance to Phytophthora root rot in two diploid alfalfa populations. Crop Sci 25:693–694CrossRefGoogle Scholar
  28. Hill RR, Elgin JH (1981) Effects of number of parents on performance of alfalfa synthetics. Crop Sci 21:298–300CrossRefGoogle Scholar
  29. Hill RR, Kalton RR (1976) Current philosophies in breeding for yield. In: Barnes DK (ed) Rep 25th Alfalfa Improve Conf, Ithaca, 13–15 July. USDA-SEA, Peoria/IL, p 51Google Scholar
  30. Hill RR, Shenk JS, Barnes RF (1988) Breeding for yield and quality. In: Hanson AA, Barnes DK, Hill RR (eds) Alfalfa and alfalfa improvement Agron Monogr 29. ASA, CSSA, and SSSA, Madison, pp 809–825Google Scholar
  31. Holland JB, Bingham ET (1994) Genetic improvement for yield and fertility of alfalfa cultivars representing different eras of breeding. Crop Sci 34:953–957CrossRefGoogle Scholar
  32. Jones JS, Bingham ET (1995) Inbreeding depression in alfalfa and cross-pollinated crops. Plant Breed Rev 13:209–233Google Scholar
  33. Kaló P, Endre G, Zimányi L, Csanádi G, Kiss GB (2000) Construction of an improved linkage map of diploid alfalfa (Medicago sativa). TAG 100:641–657CrossRefGoogle Scholar
  34. Kehr WR, Gardner CO (1960) Genetic variability in Ranger alfalfa. Agron J 52:41–44CrossRefGoogle Scholar
  35. Kidwell KK, Osborn TC (1992) Simple plant DNA isolation procedures. In: Beckman J, Osborn TC (eds) Plant genomes: methods for genetic and physical mapping. Kluwer, Dordrecht, pp 1–13Google Scholar
  36. Kimbeng CA, Bingham ET (1998a) Population improvement in alfalfa: fertility and S1 forage yield performance in original and improved populations. Crop Sci 37:1509–1513CrossRefGoogle Scholar
  37. Kimbeng CA, Bingham ET (1998b) Population improvement in lucerne (Medicago sativa L.): components of inbreeding depression are different in original and improved populations. Austr J Exp Agr 38:831–836CrossRefGoogle Scholar
  38. Kimbeng CA, Bingham ET (1999) Population improvement in lucerne (Medicago sativa L.): genetic analyses in original and improved populations. Austr J Exp Agr 39:549–554CrossRefGoogle Scholar
  39. Maureira IJ, TC Osborn (2005) Molecular markers in genetics and breeding: Improvement of alfalfa (Medicago sativa L.). In: Lörz H, Wenzel G (eds) Biotechnology in Agriculture and Forestry. Molecular marker systems in plant breeding and crop improvement, vol 55. Springer-Verlag, Heidelberg, pp 139–154Google Scholar
  40. Maureira IJ, Ortega F, Campos H, Osborn TC (2004) Population structure and combining ability of diverse Medicago sativa germplasms. Theor Appl Genet 109:775–782PubMedCrossRefGoogle Scholar
  41. Miller FR, Kebede Y (1984) Genetic contributions to yield gains in sorghum, 1950 to 1980. In: Fehr WR (eds) Genetic contributions to yield gains of five major crop plants Special Publication Number 7. CSSA, Madison, pp 1–14Google Scholar
  42. Musial JM, Aitken KS, Mackie JM, Irwin JAG (2005) A genetic linkage map in autotetraploid Lucerne adapted to northern Australia, and use of the map to identify DNA markers linked to resistance to Phytophthora medicaginis. Austr J Agr Res 56:333–344CrossRefGoogle Scholar
  43. Obert DE, Skinner DZ, Stuteville DL (2000) Association of AFLP markers with mildew resistance in autotetraploid alfalfa. Mol Breed 6:287–294CrossRefGoogle Scholar
  44. Osborn TC, Brouwer DJ, Kidwell KK, Tavoletti S, Bingham ET (1998) Molecular marker applications to genetics and breeding of alfalfa. In: Brummer EC, Hill NS, Roberts CA (eds) Molecular and cellular technologies for forage improvement. CSSA special publication number 26, Madison, pp 25–31Google Scholar
  45. Pfeiffer TW, Bingham ET (1983) Improvement of fertility and herbage yield by selection within two-allele populations of tetraploid alfalfa. Crop Sci 23:633–636CrossRefGoogle Scholar
  46. Quiros CF (1982) Tetrasomic segregation for multiple alleles in alfalfa. Genetics 101:117–127PubMedGoogle Scholar
  47. Riday H, Brummer EC (2002) Forage yield heterosis in alfalfa. Crop Sci 42:716–723CrossRefGoogle Scholar
  48. Riday H, Brummer EC (2004) Morphological variation of Medicago sativa subsp falcata and their hybrid progeny. Euphytica 138:1–12CrossRefGoogle Scholar
  49. Robins JG, Bauchan GR, Brummer EC (2007a) Genetic mapping forage yield, plant height, and regrowth at multiple harvests in tetraploid alfalfa (Medicago sativa L.). Crop Sci 47:11–18CrossRefGoogle Scholar
  50. Robins JG, Luth D, Campbell TA, Bauchan GR He C, Viands RD, Hansen JL, Brummer EC (2007b) Genetic mapping of biomass production in tetraploid alfalfa. Crop Sci 47:1–10CrossRefGoogle Scholar
  51. Rotili P, Gnocchi G, Scotti C, Zannone L (1999) Some aspects of breeding methodology in alfalfa. In: Busbice T, Rotili P, Bingham T (eds) Proceedings of ‘The Alfalfa Genome’ Conf. (http://www.naaic.org/TAG/TAGpapers/rotili/rotili.html)
  52. Rowe DE, Hill RR (1981) Inter-population improvement procedures for alfalfa. Crop Sci 21:392–397CrossRefGoogle Scholar
  53. Salter R, Miller-Garvin JE, Viands DR (1994) Breeding for resistance to alfalfa root caused by Fusarium species. Crop Sci 34:1213–1217CrossRefGoogle Scholar
  54. SAS Institute (2001) SAS/STAT® Users guide, version 8.02. SAS Inst, CaryGoogle Scholar
  55. Schwab PM, Barnes DK, Sheaffer CC (1996) The relationship field winter injury and fall growth score for 251 alfalfa cultivars. Crop Sci 36:418–426CrossRefGoogle Scholar
  56. Scotti C, Pupilli F, Salvi S, Arcioni S (2000) Variation in vigour and in RFLP-estimated heterozygosity by selfing tetraploid alfalfa: new perspectives for the use of selfing in alfalfa breeding. TAG 101:120–125CrossRefGoogle Scholar
  57. Segovia-Lerma A, Murray LW, Townsend MS, Raym IM (2004) Population-based diallel analyses among nine historically recognized alfalfa germplasms. Theor Appl Genet 109:1568–1575PubMedCrossRefGoogle Scholar
  58. Sills GR, Bridges W, Al-Janabi SM, Sobral B (1995) Genetic analysis of agronomic traits in a cross between sugarcane (Saccharum officinarum L.) and its presumed progenitor (S robustum Brandes and Jews Ex Grassl). Mol Breed 1:355–363CrossRefGoogle Scholar
  59. Smith D (1961) Association of fall growth habit and winter survival in alfalfa. Agron J 41:244–251Google Scholar
  60. Sokal RR, Rohlf FJ (1995) Biometry. Freeman, New YorkGoogle Scholar
  61. Sriwatanapongse S, Wilsie CP (1968) Intra- and intervariety crosses of Medicago sativa L and Medicago falcata L. Crop Sci 8:465–466CrossRefGoogle Scholar
  62. Stanford EH (1951) Tetrasomic inheritance in alfalfa. Agron J 43:222–225CrossRefGoogle Scholar
  63. Sumberg JE, Murphy RP, Lowe CC (1983) Selection for fiber and protein concentration in a diverse alfalfa population. Crop Sci 23:11–14CrossRefGoogle Scholar
  64. Tavoletti S, Veronessi F, Osborn TC (1996) RFLP linkage map of a meiotic mutant based on an F1 population. J Hered 87:167–170Google Scholar
  65. Van Wijk AJP, Reheul D (1991) Achievements in fodder crop breeding in maritime Europe. In: Proceedings of the 16th meeting, fodder crops section, Eucarpia 13. Pudoc, Wageningin, pp 13–18Google Scholar
  66. Waldron LR (1920) First generation crosses between two alfalfa species. J Am Soc Agron 12:133–143Google Scholar
  67. Woodfield DR, Bingham ET (1995) Improvement in two-allele autotetraploid populations of alfalfa explained by accumulation of favorable alleles. Crop Sci 35:988–994CrossRefGoogle Scholar
  68. Woodfield DR, Caradus JR (1994) Genetic gain in white clover representing six decades of plant breeding. Crop Sci. 34:1205–1213CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • I. J. Maureira-Butler
    • 1
    • 2
  • J. A. Udall
    • 1
    • 3
  • T. C. Osborn
    • 1
    • 4
  1. 1.Plant Breeding and Plant Genetics Program and Department of AgronomyUniversity of WisconsinMadisonUSA
  2. 2.Agri aquaculture Nutritional Genomic Center, Plant Biotechnology UnitINIA CarillancaTemucoChile
  3. 3.Brigham Young UniversityProvoUSA
  4. 4.Seminis Vegetable seeds (A Division of Monsanto)WoodlandUSA

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