Comparative effectiveness of sugar beet microsatellite markers isolated from genomic libraries and GenBank ESTs to map the sugar beet genome
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Sugar beet (Beta vulgaris) is an important root crop for sucrose production. A study was conducted to find a new abundant source of microsatellite (SSR) markers in order to develop marker assistance for breeding. Different sources of existing microsatellites were used and new ones were developed to compare their efficiency to reveal diversity in mapping population and mapping coverage. Forty-one microsatellite markers were isolated from a B. vulgaris ssp maritima genomic library and 201 SSRs were extracted from a B. vulgaris ssp vulgaris library. Data mining was applied on GenBank B. vulgaris expressed sequence tags (ESTs), 803 EST-SSRs were identified over 19,709 ESTs. Characteristics, polymorphism and cross-species transferability of these microsatellites were compared. Based on these markers, a high density genetic map was constructed using 92 F2 individuals from a cross between a sugar and a table beet. The map contains 284 markers, spans over 555 cM and covers the nine chromosomes of the species with an average markers density of one marker every 2.2 cM. A set of markers for assignation to the nine chromosomes of sugar beet is provided.
KeywordsLinkage Group Sugar Beet Genomic Library Trinucleotide Repeat Dinucleotide Repeat
We thank K. Bounan for crossing and growing the plants, B. Devaux for primary selection of EST-SSRs on parents and its F1 and S Barnes and JF Arnaud for useful discussions. We are grateful to M. McGrath for the numerous exchanges during map construction and assignation to chromosomes. We thank Ets Florimond Desprez and the German Federal Ministry for Education and Research (BMBF Grant No 0312706A) for Financial support.
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