Theoretical and Applied Genetics

, Volume 115, Issue 6, pp 777–792 | Cite as

The reference genetic linkage map for the multinational Brassica rapa genome sequencing project

  • Su Ryun Choi
  • Graham R. Teakle
  • Prikshit Plaha
  • Jeong Hee Kim
  • Charlotte J. Allender
  • Elena Beynon
  • Zhong Yun Piao
  • Pilar Soengas
  • Tae Ho Han
  • Graham J. King
  • Guy C. Barker
  • Paul Hand
  • Derek J. Lydiate
  • Jacqueline Batley
  • David Edwards
  • Dal Hoe Koo
  • Jae Wook Bang
  • Beom-Seok Park
  • Yong Pyo Lim
Original Paper

Abstract

We describe the construction of a reference genetic linkage map for the Brassica A genome, which will form the backbone for anchoring sequence contigs for the Multinational Brassica rapa Genome Sequencing Project. Seventy-eight doubled haploid lines derived from anther culture of the F1 of a cross between two diverse Chinese cabbage (B.rapa ssp. pekinensis) inbred lines, ‘Chiifu-401-42’ (C) and ‘Kenshin-402-43’ (K) were used to construct the map. The map comprises a total of 556 markers, including 278 AFLP, 235 SSR, 25 RAPD and 18 ESTP, STS and CAPS markers. Ten linkage groups were identified and designated as R1–R10 through alignment and orientation using SSR markers in common with existing B. napus reference linkage maps. The total length of the linkage map was 1,182 cM with an average interval of 2.83 cM between adjacent loci. The length of linkage groups ranged from 81 to 161 cM for R04 and R06, respectively. The use of 235 SSR markers allowed us to align the A-genome chromosomes of B. napus with those of B. rapa ssp. pekinensis. The development of this map is vital to the integration of genome sequence and genetic information and will enable the international research community to share resources and data for the improvement of B. rapa and other cultivated Brassica species.

Supplementary material

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Su Ryun Choi
    • 1
  • Graham R. Teakle
    • 3
  • Prikshit Plaha
    • 1
    • 11
  • Jeong Hee Kim
    • 1
  • Charlotte J. Allender
    • 3
  • Elena Beynon
    • 5
  • Zhong Yun Piao
    • 2
  • Pilar Soengas
    • 10
  • Tae Ho Han
    • 6
  • Graham J. King
    • 4
  • Guy C. Barker
    • 3
  • Paul Hand
    • 3
  • Derek J. Lydiate
    • 5
  • Jacqueline Batley
    • 7
  • David Edwards
    • 7
  • Dal Hoe Koo
    • 8
  • Jae Wook Bang
    • 8
  • Beom-Seok Park
    • 9
  • Yong Pyo Lim
    • 1
  1. 1.Department of Horticulture, Genome Research CenterChungnam National UniversityDaejeonSouth Korea
  2. 2.College of HorticultureShenyang Agricultural UniversityShenyangChina
  3. 3.Warwick HRIUniversity of WarwickWarwickUK
  4. 4.Centre for Crop Genetic ImprovementRothamsted ResearchHarpendenUK
  5. 5.Saskatoon Research Centre, Agriculture and Agri-Food CanadaSaskatoonCanada
  6. 6.Department of Applied Plant ScienceChonnam National UniversityGwangjuSouth Korea
  7. 7.Plant Biotechnology Centre, Primary Industries Research VictoriaBundooraAustralia
  8. 8.Department of BiologyChungnam National UniversityDaejeonSouth Korea
  9. 9.Brassica Genomics TeamNational Institute of Agricultural BiotechnologySuwonSouth Korea
  10. 10.Misión Biológica de Galicia (CSIC)PontevedraSpain
  11. 11.Advanced Centre of Hill Bioresources and BiotechnologyHP Agricultural UniversityPalampurIndia

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