Analysis of the chromosome 2(2H) region of barley associated with the correlated traits Fusarium head blight resistance and heading date
- 277 Downloads
Fusarium head blight (FHB) is a major disease of barley (Hordeum vulgare L.) that results in reduced grain yield and quality through the accumulation of the mycotoxin deoxynivalenol (DON). Coincident QTL for FHB severity, DON concentration, and heading date (HD) map to a region of chromosome 2(2H) designated Qrgz-2H-8. It is unclear whether disease resistance at this locus is due to a pleiotropic effect of late HD by delaying the host exposure to the pathogen or a tightly linked resistance gene. The objectives of this study were to develop a set of near isogenic lines (NILs) for the Qrgz-2H-8 region and to genetically dissect the QTL region containing the coincident traits. Two NIL populations were developed consisting of F2- and F4-derived recombinants from a cross between a BC5 line carrying the donor parent (Chevron) alleles in the Qrgz-2H-8 region and the recurrent parent M69. Analysis of field and marker data from these NILs revealed that the Chevron alleles conditioning FHB resistance, late HD, and low DON concentration were successfully introgressed into the BC5 parent line and were segregating among NILs. QTL analysis of the F4-derived population showed that the HD QTL is adjacent to the FHB QTL. Furthermore, a single NIL was identified that was similar to the resistant BC5 parent for FHB severity and the early flowering parent M69 for HD. These results indicate that the relationship between FHB and HD at the Qrgz-2H-8 region is likely due to tight linkage rather than pleiotropy.
KeywordsQuantitative Trait Locus Simple Sequence Repeat Marker Marker Assisted Selection Fusarium Head Blight Chevron
We thank Ed Schiefelbein, Guillermo Velasquez, Charlie Gustus, Ruth Dill-Macky, Amar Elkkad, Karen Wennberg, Shane Heinen, Charla Hollingsworth, and Galen Thompson for technical assistance. We also thank Dr. Bingxin Zhang for establishing the FHB nursery at Zhejiang University in Hangzhou, China. This material is based upon work supported by the U.S. Department of Agriculture, under Agreement No.59-0790-4-120. This is a cooperative project with the U.S. Wheat & Barley Scab Initiative. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the U.S. Department of Agriculture. Partial support was also received from the U.S. Barley Genome Project. Additionally, we thank the University of Minnesota Super Computing Center for providing access to the software JoinMap.
- Beavis WD (1998) QTL analyses: power, precision and accuracy. In: Paterson AH (ed) Molecular analysis of complex traits. CRC, Boca Raton, FL, pp 145–161Google Scholar
- Holland JB (2004) New directions for a diverse planet. In: Proceedings for the 4th International Crop Science Congress, Brisbane, Australia, 26 September–1 October 2004Google Scholar
- Liu S, Zhang X, Pumphrey MO, Stack RW, Gill BS, Anderson JA (2006) Complex microcolinearity among wheat, rice, and barley revealed by fine mapping of the genomic region harboring a major QTL for resistance to Fusarium head blight in wheat. Functional and Integrative Genomics 6:83–89PubMedCrossRefGoogle Scholar
- Nganje WE., Johnson DD, Wilson WW, Leistritz FL, Bangsund DA, Tiapo NM ( 2001) Economic impacts of Fusarium head blight in wheat and barley:1998–2000. Agribusiness and Applied Economics Report No. 464. North Dakota State University, Fargo, NDGoogle Scholar
- Rostoks N, Ramsay L, MacKenzie K, Cardle L, Bhat PR, Roose ML, Svensson JT, Stein N, Varshney RK, Marshall DF, Graner A, Close TJ, Waugh R (2006) Recent history of artificial outcrossing facilitates whole-genome association mapping in elite inbred crop varieties. Proc Natl Acad Sci USA 103:18656–18661PubMedCrossRefGoogle Scholar
- SAS Institute (2000) The SAS system for windows, Version 8.2. SAS Inst., Cary, NCGoogle Scholar
- Schwartz PB (2003) Impact of Fusarium head blight on malting and brewing quality of barley. In: Leaonard KJ, Bushell WR (eds) Fusarium head blight of wheat and barley. APS, St. Paul, pp 395–419Google Scholar
- Steffenson BJ (2003) Fusarium head blight of barley: impact, epidemics, management, and strategies for identifying and utilizing genetic resistance. In: Leonard KJ, Bushnell WR (eds) American Phytopathological Society, St. PaulGoogle Scholar
- Van Ooijen JW, Voorrips RE (2001) Joinmap 3.0. Software for the calculation of genetic linkage maps. Plant Research International, Wageningen, The NetherlandsGoogle Scholar
- Wenzl P, Li H, Carling J, Zhou M, Raman H, Edie E, Hearnden P, Maier C, Xia L, Caig V, Ovesna J, Cakir M, Poulsen D, Wang J, Raman R, Smith KP, Muehlbauer GJ, Chalmers KJ, Kleinhofs A, Huttner E, Kilian A (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits. BMC Genomics 7:206PubMedCrossRefGoogle Scholar
- Zhu HL, Gilchrist L, Hayes P, Kleinhofs A, Kudrna D, Liu Z, Prom L, Steffenson B, Toojinda T, Vivar H (1999) Does function follow form? Principal QTLs for Fusarium head blight (FHB) resistance are coincident with QTLs for inflorescence traits and plant height in double-haploid population of barley. Theor Appl Genet 99:1221–1232CrossRefGoogle Scholar