Theoretical and Applied Genetics

, Volume 115, Issue 2, pp 265–276

High-resolution mapping of the Alp locus and identification of a candidate gene HvMATE controlling aluminium tolerance in barley (Hordeum vulgare L.)

  • Junping Wang
  • Harsh Raman
  • Meixue Zhou
  • Peter R. Ryan
  • Emmanuel Delhaize
  • Diane M. Hebb
  • Neil Coombes
  • Neville Mendham
Original Paper

DOI: 10.1007/s00122-007-0562-9

Cite this article as:
Wang, J., Raman, H., Zhou, M. et al. Theor Appl Genet (2007) 115: 265. doi:10.1007/s00122-007-0562-9

Abstract

Aluminium (Al) tolerance in barley is conditioned by the Alp locus on the long arm of chromosome 4H, which is associated with Al-activated release of citrate from roots. We developed a high-resolution map of the Alp locus using 132 doubled haploid (DH) lines from a cross between Dayton (Al-tolerant) and Zhepi 2 (Al-sensitive) and 2,070 F2 individuals from a cross between Dayton and Gairdner (Al-sensitive). The Al-activated efflux of citrate from the root apices of Al-tolerant Dayton was 10-fold greater than from the Al-sensitive parents Zhepi 2 and Gairdner. A suite of markers (ABG715, Bmag353, GBM1071, GWM165, HvMATE and HvGABP) exhibited complete linkage with the Alp locus in the DH population accounting 72% of the variation for Al tolerance evaluated as relative root elongation. These markers were used to map this genomic region in the Dayton/Gairdner population in more detail. Flanking markers HvGABP and ABG715 delineated the Alp locus to a 0.2 cM interval. Since the HvMATE marker was not polymorphic in the Dayton/Gairdner population we instead investigated the expression of the HvMATE gene. Relative expression of the HvMATE gene was 30-fold greater in Dayton than Gardiner. Furthermore, HvMATE expression in the F2:3 families tested, including all the informative recombinant lines identified between HvGABP and ABG715 was significantly correlated with Al tolerance and Al-activated citrate efflux. These results identify HvMATE, a gene encoding a multidrug and toxic compound extrusion protein, as a candidate controlling Al tolerance in barley.

Keywords

Aluminium tolerance Barley Citrate efflux Comparative mapping MATE Molecular markers 

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Junping Wang
    • 1
    • 2
  • Harsh Raman
    • 2
  • Meixue Zhou
    • 1
  • Peter R. Ryan
    • 3
  • Emmanuel Delhaize
    • 3
  • Diane M. Hebb
    • 3
  • Neil Coombes
    • 2
  • Neville Mendham
    • 4
  1. 1.Tasmanian Institute of Agricultural Research and School of Agricultural ScienceUniversity of TasmaniaKings MeadowsAustralia
  2. 2.NSW Department of Primary IndustriesWagga Wagga Agricultural InstituteWagga WaggaAustralia
  3. 3.CSIRO Plant IndustryCanberraAustralia
  4. 4.Tasmanian Institute of Agricultural Research and School of Agricultural ScienceUniversity of TasmaniaHobartAustralia

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