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Fine mapping of the recessive genic male sterility gene (Bnms3) in Brassica napus L.

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The Brassica napus oilseed rape line, 7-7365AB, is a recessive epistatic genic male sterile (RGMS) two-type line system. The sterility is controlled by two pairs of recessive duplicate genes (Bnms3 and Bnms4) and one pair of recessive epistatic inhibitor gene (Bnrf). Homozygosity at the Bnrf locus (Bnrfrf) inhibits the expression of the two recessive male sterility genes in homozygous Bnms3ms3ms4ms4 plants and produces a male fertile phenotype. This line has a good potential for heterosis utilization but it is difficult to breed heterotic hybrids without molecular markers. To develop markers linked to the BnMs3 gene, amplified fragment length polymorphism (AFLP) technology was applied to screen the bulks of sterile and fertile individuals selected randomly from a population of near-isogenic lines (NIL) consisting of 2,000 plants. From a survey of 1,024 primer combinations, we identified 17 AFLP markers linked to the BnMs3 gene. By integrating the previous markers linked to the BnMs3 gene into the genetic map of the NIL population, two markers, EA01MC12 and EA09P06, were located on either side of the BnMs3 gene at a distance of 0.1 and 0.3 cM, respectively. In order to use the markers for male sterile line breeding, five AFLP markers, P05MG05, P03MG04, P11MG02, P05MC11250, and EA09P06, were successfully converted into sequence characterized amplified region (SCAR) markers. Two of these, P06MG04 and sR12384, were subsequently mapped on to linkage group N19 using two doubled-haploid mapping populations available at our laboratory derived from the crosses Tapidor × Ningyou7 and Quantum × No2127-17. The markers found in the present study should improve our knowledge of recessive genic male sterility (RGMS), and accelerate the development of male sterile line breeding and map-based cloning.

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  1. Chen FX, Hu BC, Li C, Li QS, Chen WS, Zhang ML (1998) Genetic studies on GMS in Brassica napus L. I. Inheritance of recessive GMS line9012A. Acta Agron Sin 24:431–438

  2. Cho YG, Blair WM, Panaud O, McCouch SR (1996) Cloning and mapping of variety-specific rice genomic DNA sequences: amplified fragment length polymorphisms (AFLP) from silver stained polyacrylamide gels. Genome 39:373–378

  3. Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15

  4. Foisset N, Delourme R, Barret P, Hubert N, Landry BS, Renard M (1996) Molecular-mapping analysis in Brassica napus using isozyme, RAPD and RFLP markers on a doubled-haploid progeny. Theor Appl Genet 93:1017–1025

  5. Fu TD, Tu JX (2002) Present situation and prospects on the research and utilization of hybrid rapeseed (in Chinese). In: Liu HL (ed) Analects of crop breeding. China Agricultural University Press, Beijing, pp 235–250

  6. Hou GZ, Wang H, Zhang RM (1990) Genetic study on genic male sterility (GMS) material No. 117A in Brassica napus L. (in Chinese). Oil Crop China 2:7–10

  7. Ke LP, Sun YQ, Hong DF, Liu PW, Yang GS (2005) Identification of AFLP markers linked to one recessive genic male sterility gene in oilseed rape, Brassica napus. Plant Breed 124:367–370

  8. Ke LP, Sun YQ, Liu PW, Yang GS (2004) Identification of AFLP fragments linked to one recessive genic male sterility (RGMS) in rapeseed (Brassica napus L.) and conversion to SCAR markers for marker-aided selection. Euphytica 138:163–168

  9. Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175

  10. Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln S, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

  11. Li SL, Qian YX, Wu ZH (1985) Genetic study on genic male sterility and its utilization in Brassica napus L. (in Chinese). Acta Agric Shanghai 1:1–12

  12. Li SL, Zhou XR, Zhou ZJ, Qian YX (1990) Inheritance of genetic male sterility (GMS) and its utilization in rape (Brassica napus L.) (in Chinese). Crop Res 4:27–32

  13. Lincoln S, Daly M, Lander E (1992) Constructing genetic maps with MAPMAKER/EXP 3.0. Whitehead institute technical report, 3rd edn. Whitehead Technical Institute, Cambridge, MA

  14. Lowe AJ, Jones AE, Raybould AF, Trick M, Moule CJ, Edwards KJ (2002) Transferability and genome specificity of a new set of microsatellite primers among Brassica species of the U triangle. Mol Ecol Notes 2:7–11

  15. Lu GY, Yang GS, Fu TD (2001) Silver stained AFLP—a novel assay for DNA fingerprinting in Brassica napus (in Chinese). J Huazhong Agric Univ 20:413–415

  16. Lu GY, Yang GS, Fu TD (2004) Molecular mapping of a dominant genic male sterility gene (Ms) in rapeseed (Brassica napus L.). Plant Breed 123:262–265

  17. Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci 88:9828–9832

  18. Mienie CM, Liebenberg MM, Pretorius ZA, Miklas PN (2005) SCAR markers linked to the common bean rust resistance gene Ur-13. Theor Appl Genet 111:972–979

  19. Negi MS, Devic M, Delseny M, Lakshmikumaran M (2000) Identification of AFLP fragments linked to seed coat colour in Brassica juncea and conversion to a SCAR marker for rapid selection. Theor Appl Genet 101:146–152

  20. Piao ZY, Deng YQ, Choi SR, Park YJ, Lim YP (2004) SCAR and CAPS mapping of CRb, a gene conferring resistance to Plasmodiophora brassicae in Chinese cabbage (Brassica rapa ssp. pekinensis). Theor Appl Genet 108:1458–1465

  21. Qiu D, Morgan C, Shi J, Long Y, Liu J, Li R, Zhuang X, Wang Y, Tan X, Dietrich E, Weihmann T, Everett C, Vanstraelen S, Beckett P, Fraser F, Trick M, Barnes S, Wilmer J, Schmidt R, Li J, Li D, Meng J, Bancroft I (2006) A comparative linkage map of oilseed rape and its use for QTL analysis of seed oil and erucic acid content. Theor Appl Genet 114(1):67–80

  22. Song LQ, Fu TD, Tu JX, Ma CZ, Yang GS (2006) Molecular validation of multiple allele inheritance for dominant genic male sterility gene in Brassica napus L. Theor Appl Genet 113:55–62

  23. Tu JX, Fu TD, Zheng YL (1997) Analysis on inheritance and isolocus of the rapeseed GMS 90-2441A (B. napus L.). J Huazhong Agric Univ 16:255–258

  24. Vos P, Hogers R, Bleeker M, Reijians M, Van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414

  25. Yi b, Chen YL, Lei SL, Tu JX, Fu TD (2006) Fine mapping of the recessive genic male- sterile gene (Bnms1) in Brassica napus L. Theor Appl Genet 113(4):643–650

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The authors wish to thank Prof. Meng and Dr. Chen for providing DNA of DH populations. This research was financed by the funds from the High-tech program “863”(20060110z1093), the High-tech program “863”(2006AA10A), the Program for Changjiang Scholar and Innovative Research Team in university (IRT0442), and the Program for “948” (2003-Q04).

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Correspondence to Jinxing Tu.

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Communicated by M. Kearsey.

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Huang, Z., Chen, Y., Yi, B. et al. Fine mapping of the recessive genic male sterility gene (Bnms3) in Brassica napus L.. Theor Appl Genet 115, 113–118 (2007). https://doi.org/10.1007/s00122-007-0547-8

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  • Amplify Fragment Length Polymorphism
  • Simple Sequence Repeat Marker
  • Sequence Characterize Amplify Region Marker
  • Fertile Individual
  • Recessive Genic Male Sterility