Theoretical and Applied Genetics

, Volume 115, Issue 1, pp 35–46

Over-expression of a LEA gene in rice improves drought resistance under the field conditions

  • Benze Xiao
  • Yuemin Huang
  • Ning Tang
  • Lizhong Xiong
Original Paper

DOI: 10.1007/s00122-007-0538-9

Cite this article as:
Xiao, B., Huang, Y., Tang, N. et al. Theor Appl Genet (2007) 115: 35. doi:10.1007/s00122-007-0538-9

Abstract

Late embryogenesis abundant (LEA) proteins have been implicated in many stress responses of plants. In this report, a LEA protein gene OsLEA3-1 was identified and over-expressed in rice to test the drought resistance of transgenic lines under the field conditions. OsLEA3-1 is induced by drought, salt and abscisic acid (ABA), but not by cold stress. The promoter of OsLEA3-1 isolated from the upland rice IRAT109 exhibits strong activity under drought- and salt-stress conditions. Three expression constructs consisting of the full-length cDNA driven by the drought-inducible promoter of OsLEA3-1 (OsLEA3-H), the CaMV 35S promoter (OsLEA3-S), and the rice Actin1 promoter (OsLEA3-A) were transformed into the drought-sensitive japonica rice Zhonghua 11. Drought resistance pre-screening of T1 families at anthesis stage revealed that the over-expressing families with OsLEA3-S and OsLEA3-H constructs had significantly higher relative yield (yield under drought stress treatment/yield under normal growth conditions) than the wild type under drought stress conditions, although a yield penalty existed in T1 families under normal growth conditions. Nine homozygous families, exhibiting over-expression of a single-copy of the transgene and relatively low yield penalty in the T1 generation, were tested in the field for drought resistance in the T2 and T3 generations and in the PVC pipes for drought tolerance in the T2 generation. Except for two families (transformed with OsLEA3-A), all the other families (transformed with OsLEA3-S and OsLEA3-H constructs) had higher grain yield than the wild type under drought stress in both the field and the PVC pipes conditions. No significant yield penalty was detected for these T2 and T3 families. These results indicate that transgenic rice with significantly enhanced drought resistance and without yield penalty can be generated by over-expressing OsLEA3-1 gene with appropriate promoters and following a bipartite (stress and non-stress) in-field screening protocol.

Keywords

Abiotic stress Grain yield Promoter Oryza sativa 

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Benze Xiao
    • 1
  • Yuemin Huang
    • 1
  • Ning Tang
    • 1
  • Lizhong Xiong
    • 1
  1. 1.National Center of Plant Gene Research (Wuhan), National Key Laboratory of Crop Genetic ImprovementHuazhong Agricultural UniversityWuhanChina

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