Theoretical and Applied Genetics

, Volume 114, Issue 4, pp 755–764 | Cite as

Assessment of the importance of α-amylase inhibitor-2 in bruchid resistance of wild common bean

  • Keito Nishizawa
  • Masayoshi Teraishi
  • Shigeru Utsumi
  • Masao IshimotoEmail author
Original Paper


Both α-amylase inhibitor-2 (αAI-2) and arcelin have been implicated in resistance of wild common bean (Phaseolus vulgaris L.) to the Mexican bean weevil (Zabrotes subfasciatus Boheman). Near isogenic lines (NILs) for arcelin 1–5 were generated by backcrossing wild common bean accessions with a cultivated variety. Whereas seeds of a wild accession (G12953) containing both αAI-2 and arcelin 4 were completely resistant to Z. subfasciatus, those of the corresponding NIL were susceptible to infestation, suggesting that the principal determinant of resistance was lost during backcrossing. Three independent lines of transgenic azuki bean [Vigna angularis (Willd.) Ohwi and Ohashi] expressing αAI-2 accumulated high levels of this protein in seeds. The expression of αAI-2 in these lines conferred protection against the azuki bean weevil (Callosobruchus chinensis L.), likely through inhibition of larval digestive α-amylase. However, although the seed content of αAI-2 in these transgenic lines was similar to that in a wild accession of common bean (G12953), it did not confer a level of resistance to Z. subfasciatus similar to that of the wild accession. These results suggest that αAI-2 alone does not provide a high level of resistance to Z. subfasciatus. However, αAI-2 is an effective insecticidal protein with a spectrum of activity distinct from that of αAI-1, and it may prove beneficial in genetic engineering of insect resistance in legumes.


Common Bean Wild Accession Azuki Bean Wild Parent Tepary Bean 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This research was supported by the Project for Development of Innovative Transgenic Plants of the Ministry of Agriculture, Forestry, and Fisheries of Japan. We thank Koichi Fujii (University of Tsukuba) for providing colonies of Z. subfasciatus, Elizabeth E. Hood (ProdiGene, College Station, TX) for providing A. tumefaciens strain EHA105, and Yumi Nakamoto for technical assistance.


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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Keito Nishizawa
    • 1
    • 2
  • Masayoshi Teraishi
    • 3
  • Shigeru Utsumi
    • 2
  • Masao Ishimoto
    • 1
    Email author
  1. 1.National Agricultural Research Center for Hokkaido RegionSapporoJapan
  2. 2.Laboratory of Food Quality Design and Development, Graduate School of AgricultureKyoto UniversityUjiJapan
  3. 3.Laboratory of Plant Production Control, Graduate School of AgricultureKyoto UniversityTakatsukiJapan

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