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Theoretical and Applied Genetics

, Volume 114, Issue 2, pp 341–349 | Cite as

A microsatellite marker based linkage map of tobacco

  • Gregor Bindler
  • Rutger van der Hoeven
  • Irfan Gunduz
  • Jörg Plieske
  • Martin Ganal
  • Luca Rossi
  • Ferruccio Gadani
  • Paolo DoniniEmail author
Original Paper

Abstract

We report the first linkage map of tobacco (Nicotiana tabacum L.) generated through microsatellite markers. The microsatellite markers were predominantly derived from genomic sequences of the Tobacco Genome Initiative (TGI) through bioinformatics screening for microsatellite motives. A total of 684 primer pairs were screened for functionality in a panel of 16 tobacco lines. Of those, 637 primer pairs were functional. Potential parents for mapping populations were evaluated for their polymorphism level through genetic similarity analysis. The similarity analysis revealed that the known groups of tobacco varieties (Burley, Flue-cured, Oriental and Dark) form distinct clusters. A mapping population, based on a cross between varieties Hicks Broad Leaf and Red Russian, and consisting of 186 F2 individuals, was selected for mapping. A total of 282 functional microsatellite markers were polymorphic in this population and 293 loci could be mapped together with the morphological trait flower color. Twenty-four tentative linkage groups spanning 1,920 cM could be identified. This map will provide the basis for the genetic mapping of traits in tobacco and for further analyses of the tobacco genome.

Keywords

Linkage Group Microsatellite Marker Mapping Population Tobacco Genome Solanaceous Species 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

Prof. Ramsey Lewis is acknowledged for providing the seeds for the mapping population. TraitGenetics GmbH is thankful to Anika Küttner and Doris Kriseleit for their qualified technical assistance. Furthermore, the efforts of Alec Hayes (Philip Morris USA), C. Opperman and S. Lommel (both North Carolina State University) within the TGI are acknowledged.

Supplementary material

122_2006_437_MOESM1_ESM.doc (407 kb)
Supplementary material

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Gregor Bindler
    • 1
  • Rutger van der Hoeven
    • 1
  • Irfan Gunduz
    • 2
  • Jörg Plieske
    • 3
  • Martin Ganal
    • 3
  • Luca Rossi
    • 1
  • Ferruccio Gadani
    • 1
  • Paolo Donini
    • 1
    Email author
  1. 1.Applied Research DepartmentPhilip Morris International, R&DNeuchâtelSwitzerland
  2. 2.Philip Morris USA, Research CenterRichmondUSA
  3. 3.TraitGenetics GmbHGaterslebenGermany

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