A comparative linkage map of oilseed rape and its use for QTL analysis of seed oil and erucic acid content
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We have developed a new DH mapping population for oilseed rape, named TNDH, using genetically and phenotypically diverse parental lines. We used the population in the construction of a high stringency genetic linkage map, consisting of 277 loci, for use in quantitative genetic analysis. A proportion of the markers had been used previously in the construction of linkage maps for Brassica species, thus permitting the alignment of maps. The map includes 68 newly developed Sequence Tagged Site (STS) markers targeted to the homologues of defined genes of A. thaliana. The use of these markers permits the alignment of our linkage map with the A. thaliana genome sequence. An additional 74 loci (31 newly developed STS markers and 43 loci defined by SSR and RFLP markers that had previously been used in published linkage maps) were added to the map. These markers increased the resolution of alignment of the newly constructed linkage map with existing Brassica linkage maps and the A. thaliana genome sequence. We conducted field trials with the TNDH population at two sites, and over 2 years, and identified reproducible QTL for seed oil content and erucic acid content. The results provide new insights into the genetic control of seed oil and erucic acid content in oilseed rape, and demonstrate the utility of the linkage map and population.
KeywordsQuantitative Trait Locus Bacterial Artificial Chromosome Double Haploid Oilseed Rape Erucic Acid
The TNDH population is available from the corresponding author. Marker scoring data, and the latest version of the linkage map, are available via the Web site of the senior authors (http://www.jic.bbsrc.ac.uk/staff/ian-bancroft/research_page3.htm#linkage and http://croplab.hzau.edu.cn/brassica/). We thank the EU Framework 5 Programme for financial support through INCO-DEV grant ICA4-CT-10067 and the National Grand Fundamental Research Program of Education of China under Grant No. 104171.
- Hu JG, Vick BA (2003) Target region amplification polymorphism: a novel marker technique for plant genotyping. Plant Mol Biol Rep 21:289–294Google Scholar
- Lowe AJ, Jones AE, Raybould AF, Trick M, Moule CL, Edwards KJ (2002) Transferability and genome specificity of a new set of microsatellite primers among Brassica species of the U triangle. Mol Ecol Notes 2:7–11Google Scholar
- Lühs W, Friedt W (1993) Nonfood uses of vegetable oils and fatty acids. In: Murphy DJ (ed) Designer oil crops: breeding, processing and biotechnology. VCH, Cambridge, pp 73–130Google Scholar
- Lydiate D, Sharpe A (2003) Aliging genetic maps of Brassica napus Using microsatellite markers. In: Plant & animal genomes XI conference, p 473Google Scholar
- Meng J, Sharpe A, Bowman C, Tian Z, Qian X, Lydiate D (1996) Genetic diversity of Brassica napus accessions mainly from China detected with RFLP markers. Chin J Genet 23(3):221–232Google Scholar
- Mika V, Tillmann P, Koprna R, Nerusil P, Kucera V (2003) Fast prediction of quality parameters in whole seeds of oilseed rape (Brassica napus L.). Plant Soil Environ 49:141–145Google Scholar
- Szewc-McFadden AK, Kresovich S, Bliek SM, Mitchell SE, McFerson JR (1996) Identification of polymorphic, conserved simple sequence repeats (SSRs) in cultivated Brassica species. Theor Appl Genet 93:534–538Google Scholar
- Van Ooijen JW (2004) MapQTL 5, Software for the mapping of quantitative trait loci in experimental populations. Kyazma BV, WageningenGoogle Scholar