Molecular detection of QTLs for agronomic and quality traits in a doubled haploid population derived from two Canadian wheats (Triticum aestivum L.)
- 1.3k Downloads
Development of high-yielding wheat varieties with good end-use quality has always been a major concern for wheat breeders. To genetically dissect quantitative trait loci (QTLs) for yield-related traits such as grain yield, plant height, maturity, lodging, test weight and thousand-grain weight, and for quality traits such as grain and flour protein content, gluten strength as evaluated by mixograph and SDS sedimentation volume, an F1-derived doubled haploid (DH) population of 185 individuals was developed from a cross between a Canadian wheat variety “AC Karma” and a breeding line 87E03-S2B1. A genetic map was constructed based on 167 marker loci, consisting of 160 microsatellite loci, three HMW glutenin subunit loci: Glu-A1, Glu-B1 and Glu-D1, and four STS-PCR markers. Data for investigated traits were collected from three to four environments in Manitoba, Canada. QTL analyses were performed using composite interval mapping. A total of 50 QTLs were detected, 24 for agronomic traits and 26 for quality-related traits. Many QTLs for correlated traits were mapped in the same genomic regions forming QTL clusters. The largest QTL clusters, consisting of up to nine QTLs, were found on chromosomes 1D and 4D. HMW glutenin subunits at Glu-1 loci had the largest effect on breadmaking quality; however, other genomic regions also contributed genetically to breadmaking quality. QTLs detected in the present study are compared with other QTL analyses in wheat.
KeywordsPlant Height Doubled Haploid Agronomic Trait Doubled Haploid Line Composite Interval Mapping
We would like to thank the breeding teams at Winnipeg and Brandon for the field experiments and evaluations, Elsa Reimer for excellent assistance in genotyping and Sheila Woods for statistical analysis advice. Review of the manuscript and useful comments provided by Andrzej Walichnowski, and Drs Curt McCartney and David De Koeyer are greatly acknowledged. X. Q. Huang received a visiting fellowship from the National Science and Engineering Research Council of Canada (NSERC). This research was supported in part by an NSERC post-graduate scholarship to N. Radovanovic. This publication is Agriculture and Agri-Food Canada contribution no. 1921.
- American Association of Cereal Chemists (2000) Approved Methods of the AACC, 10th edn St. Paul. Methods 26-95A, 39-25, and 56-70Google Scholar
- Basten CJ, Weir BS, Zeng Z-B (2001) QTL Cartographer, version 1.15. Department of Statistics, North Carolina State University, RaleighGoogle Scholar
- Knox RE, De Pauw RM, McCaig TN, Clarke JM, McLeod JG, Fernandez MR (1995) AC Karma white spring wheat. Can J Plant Sci 75: 899–901Google Scholar
- Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175Google Scholar
- Lukow OM, Forsyth SA, Payne PI (1992) Over-production of HMW glutenin subunits coded on chromosome 1B in common wheat, Triticum aestivum. J Genet Breed 46:187–192Google Scholar
- McIntosh RA, Hart GE, Devos KM, Gale MD, Rogers WJ (1998) Catalogue of gene symbols for wheat. In: Slinkard AE (ed) Proceedings of the 9th International Wheat Genet Symp, vol 5. University Extension Press, University of Saskatchewan, CanadaGoogle Scholar
- Röder MS, Huang XQ, Ganal MW (2004) Wheat microsatellites in plant breeding—potential and implications. In: Lörz H, Wenzel G (eds) Biotechnology in agriculture and forestry, vol 55, molecular marker systems in plant breeding and crop improvement. Springer, Berlin Heidelberg New York, pp 255–266Google Scholar
- Sourdille P, Singh S, Cadalen T, Brown-Guedira GL, Gay G, Qi L, Gill BS, Dufour P, Murigneux A, Bernard M (2004) Microsatellite-based deletion bin system for the establishment of genetic–physical map relationships in wheat (Triticum aestivum L.). Funct Integr Genomics 4:12–25PubMedCrossRefGoogle Scholar
- Wang S, Basten CJ, Zeng Z-B (2004) Windows QTL Cartographer 2.0. Department of Statistics, North Carolina State University, Raleigh (http://www.statgen.ncsu.edu/qtlcart/WQTLCart.htm)