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Genetic mapping reveals a single major QTL for bacterial wilt resistance in Italian ryegrass (Lolium multiflorum Lam.)

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Abstract

Bacterial wilt caused by Xanthomonas translucens pv. graminis (Xtg) is a major disease of economically important forage crops such as ryegrasses and fescues. Targeted breeding based on seedling inoculation has resulted in cultivars with considerable levels of resistance. However, the mechanisms of inheritance of resistance are poorly understood and further breeding progress is difficult to obtain. This study aimed to assess the relevance of the seedling screening in the glasshouse for adult plant resistance in the field and to investigate genetic control of resistance to bacterial wilt in Italian ryegrass (Lolium multiflorum Lam.). A mapping population consisting of 306 F1 individuals was established and resistance to bacterial wilt was assessed in glasshouse and field experiments. Highly correlated data (r = 0.67–0.77, P < 0.01) between trial locations demonstrated the suitability of glasshouse screens for phenotypic selection. Analysis of quantitative trait loci (QTL) based on a high density genetic linkage map consisting of 368 amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers revealed a single major QTL on linkage group (LG) 4 explaining 67% of the total phenotypic variance (Vp). In addition, a minor QTL was observed on LG 5. Field experiments confirmed the major QTL on LG 4 to explain 43% (in 2004) to 84% (in 2005) of Vp and also revealed additional minor QTLs on LG 1, LG 4 and LG 6. The identified QTLs and the closely linked markers represent important targets for marker-assisted selection of Italian ryegrass.

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Acknowledgements

We would like to acknowledge CRCMPB, Australia and NILGS, Japan for granting research licences for the use of SSR markers, Dr. F.X. Schubiger for providing the Xtg 29 isolate and P. Streckeisen and Y. Häfele for excellent technical support. We further thank Prof. Dr. agr. H.F. Utz and Dr. C.-C. Schön, University of Hohenheim for support regarding field trial design, QTL and statistical analyses. The present study was funded by the Swiss National Science Foundation (grant 3100-065417) and kindly supported by grants from the “Bundesministerium für Verbraucherschutz, Ernährung und Landwirtschaft” (BMVEL) and “Gemeinschaft zur Förderung der privaten deutschen Pflanzenzüchtung e.V.” (GFP, grant 01 HS 006).

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  1. Agroscope Reckenholz-Tänikon Research Station ART, Reckenholzstr. 191, 8046, Zurich, Switzerland

    Bruno Studer, Beat Boller, Doris Herrmann, Franco Widmer & Roland Kölliker

  2. State Plant Breeding Institute, University of Hohenheim, 70593, Stuttgart, Germany

    Eva Bauer & Ulrich K. Posselt

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  1. Bruno Studer
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  2. Beat Boller
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  3. Doris Herrmann
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  6. Franco Widmer
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  7. Roland Kölliker
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Correspondence to Roland Kölliker.

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Communicated by T. Lübberstedt

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Studer, B., Boller, B., Herrmann, D. et al. Genetic mapping reveals a single major QTL for bacterial wilt resistance in Italian ryegrass (Lolium multiflorum Lam.). Theor Appl Genet 113, 661–671 (2006). https://doi.org/10.1007/s00122-006-0330-2

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