Theoretical and Applied Genetics

, Volume 113, Issue 2, pp 186–195

EST sequencing and phylogenetic analysis of the model grass Brachypodium distachyon

  • John P. Vogel
  • Yong Qiang Gu
  • Paul Twigg
  • Gerard R. Lazo
  • Debbie Laudencia-Chingcuanco
  • Daniel M. Hayden
  • Teresa J. Donze
  • Lindsay A. Vivian
  • Boryana Stamova
  • Devin Coleman-Derr
Original Paper

Abstract

Brachypodium distachyon (Brachypodium) is a temperate grass with the physical and genomic attributes necessary for a model system (small size, rapid generation time, self-fertile, small genome size, diploidy in some accessions). To increase the utility of Brachypodium as a model grass, we sequenced 20,440 expressed sequence tags (ESTs) from five cDNA libraries made from leaves, stems plus leaf sheaths, roots, callus and developing seed heads. The ESTs had an average trimmed length of 650 bp. Blast nucleotide alignments against SwissProt and GenBank non-redundant databases were performed and a total of 99.9% of the ESTs were found to have some similarity to existing protein or nucleotide sequences. Tentative functional classification of 77% of the sequences was possible by association with gene ontology or clusters of orthologous group’s index descriptors. To demonstrate the utility of this EST collection for studying cell wall composition, we identified homologs for the genes involved in the biosynthesis of lignin subunits. A subset of the ESTs was used for phylogenetic analysis that reinforced the close relationship of Brachypodium to wheat and barley.

Supplementary material

122_2006_285_MOESM1_ESM.fas.
Supplementary material

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • John P. Vogel
    • 1
  • Yong Qiang Gu
    • 1
  • Paul Twigg
    • 2
  • Gerard R. Lazo
    • 1
  • Debbie Laudencia-Chingcuanco
    • 1
  • Daniel M. Hayden
    • 1
  • Teresa J. Donze
    • 2
  • Lindsay A. Vivian
    • 2
  • Boryana Stamova
    • 1
  • Devin Coleman-Derr
    • 1
  1. 1.USDA Western Regional Research CenterAlbanyUSA
  2. 2.University of Nebraska at KearneyKearneyUSA

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