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Theoretical and Applied Genetics

, Volume 113, Issue 1, pp 163–175 | Cite as

Genetic variations of cell wall digestibility related traits in floral stems of Arabidopsis thaliana accessions as a basis for the improvement of the feeding value in maize and forage plants

  • Y. BarrièreEmail author
  • D. Denoue
  • M. Briand
  • M. Simon
  • L. Jouanin
  • M. Durand-Tardif
Original Paper

Abstract

Floral stems of Arabidopsis thaliana accessions were used as a model system relative to forage plant stems in genetic variation studies of lignin content and cell wall digestibility related traits. Successive investigations were developed in a core collection of 24 Arabidopsis accessions and in a larger collection of 280 accessions. Significant genetic variation for lignin content in the cell wall, and for the two in vitro cell wall digestibility investigated traits, were found both in the core collection and in the large collection. Genotype × environment interactions, investigated in the core collection, were significant with a few genotypes contributing greatly to interactions, based on ecovalence value estimates. In the core collection, genotypes 42AV, 224AV, and 8AV had low cell wall digestibility values, whatever be the environmental conditions. Genotype 157AV, observed only in one environment, also appeared to have a low cell wall digestibility. Conversely, genotypes 236AV, 162AV, 70AV, 101AV, 83AV had high cell wall digestibility values, genotype 83AV having a slightly greater instability across differing environments than others. The well-known accession Col-0 (186AV) appeared with a medium level of cell wall digestibility and a weak to medium level of interaction between environments. The ranges of variation in cell wall digestibility traits were higher in the large collection than in the core collection of 24 accessions, these results needing confirmation due to the lower number of replicates. Accessions 295AV, 148AV, and 309AV could be models for low stem cell wall digestibility values, with variable lignin content. Similarly, accessions 83AV and 162AV, already identified from the study of the core collection, and five accessions (6AV, 20AV, 91AV, 114AV, and 223AV) could be models for high stem cell wall digestibility values. The large variations observed between Arabidopsis accessions for both lignin content and cell wall digestibility in floral stems have strengthened the use this species as a powerful tool for discovering genes involved in cell wall biosynthesis and lignification of dicotyledons forage plants. Investigations of this kind might also be applicable to monocotyledons forage plants due to the basic similarity of the genes involved in the lignin pathway of Angiosperms and the partial homology of the cell wall composition and organization of the mature vascular system in grasses and Arabidopsis.

Keywords

Lignin Lignin Content Core Collection Cinnamyl Alcohol Dehydrogenase Cell Wall Biosynthesis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This work was partly supported by a grant from the Génoplante—GABI Arabidopsis thaliana program coordinated by Mylène Durand-Tardif (INRA Versailles). Simone Allerit, Christiane Minault, René Minault, and Pascal Vernoux (INRA Lusignan) are thanked for plant cropping, sample preparation and biochemical analyses. We are grateful towards two anonymous reviewers for their fruitful criticisms. We also acknowledge the Cornell University Forage Breeding Project that puts the alfalfa pectin NIRS calibration available.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Y. Barrière
    • 1
    Email author
  • D. Denoue
    • 1
  • M. Briand
    • 1
  • M. Simon
    • 2
  • L. Jouanin
    • 3
  • M. Durand-Tardif
    • 2
  1. 1.INRA, Unité de Génétique et Amélioration des Plantes FourragèresLusignanFrance
  2. 2.INRA, Station de Génétique et Amélioration des PlantesVersailles CedexFrance
  3. 3.INRA, Laboratoire de Biologie CellulaireVersailles CedexFrance

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