Comparative field performance over 3 years and two sites of transgenic wheat lines expressing HMW subunit transgenes
- 149 Downloads
A series of transgenic wheat lines expressing additional high molecular weight (HMW) subunit genes and the corresponding control lines were grown in replicate field trials at two UK sites (Rothamsted Research, approximately 50 km north of London and Long Ashton, near Bristol) over 3 years (1998, 1999, 2000), with successive generations of the transgenic lines (T3, T4, T5) being planted. Four plots from each site were used to determine grain dry weight, grain nitrogen, dough strength (measured as peak resistance by Mixograph analysis) and the expression levels of the endogenous and “added” subunits. Detailed statistical analyses showed that the transgenic and non-transgenic lines did not differ in terms of stability of HMW subunit gene expression or in stability of grain nitrogen, dry weight or dough strength, either between the 3 years or between sites and plots. These results indicate that the transgenic and control lines can be regarded as substantially equivalent in terms of stability of gene expression between generations and environments.
KeywordsTransgenic Line Linear Discriminant Analysis Control Line Endogenous Gene Dough Strength
Rothamsted Research received grant-aided support from the Biotechnology and Biological Sciences Research Council of the United Kingdom. Part of this work was supported by a grant to Long Ashton Research Station from Zeneca Ltd (subsequently Syngenta) (1999–2002).
- Altpeter F, Baisakh N, Beachy R, Bock R, Capell T, Christou P, Daniell H, Datta K, Datta S, Dix PJ, Fauquet C, Huang N, Kohli A, Mooibroek H, Nicholson L, Nguyen TT, Nugent G, Raemakers K, Romano A, Somers DA, Stoger E, Taylor N, Visser R (2005) Particle bombardment and the genetic enhancement of crops: myths and realities. Mol Breed 15:305–327CrossRefGoogle Scholar
- Barcelo P, Rasco-Gaunt S, Thorpe C, Lazzeri PA (2001) Transformation and gene expression. In: Shewry PR, Lazzeri PA, Edwards KJ (eds) Advances in botanical research incorporating advances in plant pathology, vol 34, pp 59–126, (Series ed J.A Callow)Google Scholar
- Mardia KV, Kent JT, Bibby JM (1979) Multivariate analysis. Academic, London, pp 521Google Scholar
- Rath CR, Gras PW, Wrigley CW, Walker C.E. (1990) Evaluation of dough properties from two grams of flour using the Mixograph principle. Cereal Foods World 35:572–574Google Scholar
- Shewry PR, Tatham AS, Fido RJ (1995) Separation of plant proteins by electrophoresis. In: Jones H (ed) Methods in molecular biology—plant gene transfer and expression protocols, vol 49. Humana Press, Totowa, pp 399–422Google Scholar
- Shewry PR, Halford NG, Tatham AS, Popineau Y, Lafiandra D, Belton PS (2003) The high molecular weight subunits of wheat glutenin and their role in determining wheat processing properties. Adv Food Nutr Res 45:221–302Google Scholar
- Walker CE, Hazelton JL (1996) Dough rheological tests. Cereal Foods World 41:23–28Google Scholar