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Theoretical and Applied Genetics

, Volume 113, Issue 1, pp 73–80 | Cite as

Complete assignment of the chromosomes of Gossypium hirsutum L. by translocation and fluorescence in situ hybridization mapping

  • Kai Wang
  • Xianliang Song
  • Zhiguo Han
  • Wangzhen Guo
  • John Z. Yu
  • Jing Sun
  • Jiaju Pan
  • Russell J. Kohel
  • Tianzhen ZhangEmail author
Original Paper

Abstract

Significant progress has been made in the construction of genetic maps in the tetraploid cotton Gossypium hirsutum. However, six linkage groups (LGs) have still not been assigned to specific chromosomes, which is a hindrance for integrated genetic map construction. In the present research, specific bacterial artificial chromosome (BAC) clones constructed in G. hirsutum acc. TM-1 for these six LGs were identified by screening the BAC library using linkage group-specific simple-sequence repeats markers. These BAC clones were hybridized to ten translocation heterozygotes of G. hirsutum. L as BAC-fluorescence in situ hybridization probes, which allowed us to assign these six LGs A01, A02, A03, D02, D03, and D08 to chromosomes 13, 8, 11, 21, 24, and 19, respectively. Therefore, the 13 homeologous chromosome pairs have been established, and we have proposed a new chromosome nomenclature for tetraploid cotton.

Keywords

Bacterial Artificial Chromosome Bacterial Artificial Chromosome Clone Bacterial Artificial Chromosome Library Tetraploid Cotton Positive Bacterial Artificial Chromosome Clone 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This program was supported financially in part by Program for Changjiang Scholars and Innovative Research Team in University, the Ministry of Education, National Science Foundation in China (30070483, 30270806) Visiting Scholar Foundation of Key Laboratory in University (ZW2002003), and China Postdoctoral Science Foundation (2005037736). We thank Dr. X E Wang and Ms B Zhou, NAU, China for technical assistance.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Kai Wang
    • 1
  • Xianliang Song
    • 1
  • Zhiguo Han
    • 1
  • Wangzhen Guo
    • 1
  • John Z. Yu
    • 2
  • Jing Sun
    • 1
  • Jiaju Pan
    • 1
  • Russell J. Kohel
    • 2
  • Tianzhen Zhang
    • 1
    Email author
  1. 1.National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Research InstituteNanjing Agricultural UniversityNanjingChina
  2. 2.USDA-ARS, Southern Plains Agricultural Research CenterCrop Germplasm Research UnitCollege StationUSA

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