Theoretical and Applied Genetics

, Volume 113, Issue 1, pp 73–80 | Cite as

Complete assignment of the chromosomes of Gossypium hirsutum L. by translocation and fluorescence in situ hybridization mapping

  • Kai Wang
  • Xianliang Song
  • Zhiguo Han
  • Wangzhen Guo
  • John Z. Yu
  • Jing Sun
  • Jiaju Pan
  • Russell J. Kohel
  • Tianzhen ZhangEmail author
Original Paper


Significant progress has been made in the construction of genetic maps in the tetraploid cotton Gossypium hirsutum. However, six linkage groups (LGs) have still not been assigned to specific chromosomes, which is a hindrance for integrated genetic map construction. In the present research, specific bacterial artificial chromosome (BAC) clones constructed in G. hirsutum acc. TM-1 for these six LGs were identified by screening the BAC library using linkage group-specific simple-sequence repeats markers. These BAC clones were hybridized to ten translocation heterozygotes of G. hirsutum. L as BAC-fluorescence in situ hybridization probes, which allowed us to assign these six LGs A01, A02, A03, D02, D03, and D08 to chromosomes 13, 8, 11, 21, 24, and 19, respectively. Therefore, the 13 homeologous chromosome pairs have been established, and we have proposed a new chromosome nomenclature for tetraploid cotton.


Bacterial Artificial Chromosome Bacterial Artificial Chromosome Clone Bacterial Artificial Chromosome Library Tetraploid Cotton Positive Bacterial Artificial Chromosome Clone 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This program was supported financially in part by Program for Changjiang Scholars and Innovative Research Team in University, the Ministry of Education, National Science Foundation in China (30070483, 30270806) Visiting Scholar Foundation of Key Laboratory in University (ZW2002003), and China Postdoctoral Science Foundation (2005037736). We thank Dr. X E Wang and Ms B Zhou, NAU, China for technical assistance.


  1. Baker RJ, Longmire JL, Van Den Bussche RA (1995) Organization of repetitive elements in the upland cotton genome (Gossypium hirsutum). J Hered 86:178–185Google Scholar
  2. Beasley JO (1942) Meiotic chromosome behaviour in species hybrids, haploids, and induced polyploids of Gossypium. Genetics 27:25–54PubMedGoogle Scholar
  3. Brown MS (1980) The identification of the chromosomes of Gossypium hirsutum L. by means of translocations. J Hered 71:266–274Google Scholar
  4. Brown MS, Menzel MY, Hasenkampf CA, Naqi S (1981) Chromosome configurations and orientations in 58 heterozygous translocations in Gossypium hirsutum. J Hered 72:161–168Google Scholar
  5. Brubaker CL, Paterson AH, Wendel JF (1999) Comparative genetic mapping of allotetraploid cotton and its diploid progenitors. Genome 42:184–203CrossRefGoogle Scholar
  6. Cheng Z, Buell CR, Wing RA, Gu M, Jiang J (2001) Toward a cytological characterization of the rice genome. Genome Res 11:2133–2141CrossRefPubMedGoogle Scholar
  7. Crane CF, Price HJ, Stelly DM, Czeschin DG (1993) Identification of a homeologous chromosome pair by in situ DNA hybridization to ribosomal RNA loci in meiotic chromosomes of cotton (Gossypium hirsutum). Genome 36:1015–1022PubMedCrossRefGoogle Scholar
  8. Dong F, Song J, Naess SK, Helgeson JP, Gebhardt C, Jiang J (2000) Development and applications of a set of chromosome-specific cytogenetic DNA markers in potato. Theor Appl Genet 101:1001–1007CrossRefGoogle Scholar
  9. Endrizzi JE, Turcotte EL, Kohel RJ (1984) Qualitative genetics, cytology, and cytogenetics. In: Kohel RJ, Lewis CF (eds) Cotton. American Society of Agronomy, Madison, pp 81–129Google Scholar
  10. Endrizzi JE, Turcotte EL, Kohel RJ (1985) Genetics, cytology, and evolution of Gossypium. Adv Genet 23:271–375CrossRefGoogle Scholar
  11. Fryxell PA (1992) A revised taxonomic interpretation of Gossypium L. (Malvaceae). Rheedea 2:108–165Google Scholar
  12. Han ZG, Guo WZ, Song XL, Zhang TZ (2004) Genetic mapping of EST-derived microsatellites from the diploid Gossypium arboreumin allotetraploid cotton. Mol Gen Genomics 272:308–327CrossRefGoogle Scholar
  13. Han ZG, Wang CB, Song XL, Guo WZ, Gou JY, Li CH, Chen XY, Zhang TZ (2006) Characteristics, development and mapping of G.hirsutum derived-EST-SSRs in allotetraploid cotton. Theor Appl Genet 112:430–439CrossRefPubMedGoogle Scholar
  14. Hanson RE, Zwick MS, Choi S, Islam-Faridi MN, McKnight TD, Wing RA, Price HJ, Stelly DM (1995) Fluorescent in situ hybridization of a bacterial artificial chromosome. Genome 38:646–651PubMedCrossRefGoogle Scholar
  15. Ji YF, De Donato M, Cranel CF, Raska WA, Islam-Faridi MN, McKnight TD, Price HJ, Stelly DM (1999) New ribosomal RNA gene locations in Gossypium hirsutum mapped by meiotic FISH. Chromosoma 108:200–207CrossRefPubMedGoogle Scholar
  16. Jiang J, Gill BS, Wang GL, Ronald PC, Ward DC (1995) Metaphase and interphase fluorescence in situ hybridization mapping of the rice genome with bacterial artificial chromosomes. Proc Natl Acad Sci USA 92:4487–4491PubMedCrossRefGoogle Scholar
  17. Kim JS, Childs KL, Islam-Faridi MN, Menz MA, Klein RR, Klein PE, Price HJ, Mullet JE, Stelly DM (2002) Integrated karyotyping of sorghum by in situ hybridization of landed BACs. Genome 45:402–412CrossRefPubMedGoogle Scholar
  18. Kohel RJ (1973) Genentic nomenclature in cotton. J Hered 64:291–295Google Scholar
  19. Lacape JM, Nguyen TB, Thibivilliers S, Bojinov B, Courtois B, Cantrell RG, Burr B, Hau B (2003) A combined RFLP–SSR–AFLP map of tetraploid cotton based on a Gossypium hirsutum × Gossypium barbadense backcross population. Genome 46:612–626CrossRefPubMedGoogle Scholar
  20. Lee JA (1984) Cotton as a world crop. In: Kohel RJ, Lewis CL (eds) Cotton. Agronomy Monograph, no. 24, 1–25. Crop Science Society of America, MadisonGoogle Scholar
  21. Li G, Quiros CF (2001) Sequence-related amplified polymorphisim (SRAP), a new marker system based on a simple PCR reaction: Its application to mapping and gene tagging in Brassica. Theor Appl Genet 103:455–461CrossRefGoogle Scholar
  22. Mei M, Syed NH, Gao W, Thaxton PM, Smith CW, Stelly DM, Chen ZJ (2004) Genetic mapping and QTL analysis of fiber-related traits in cotton (Gossypium). Theor Appl Genet 108:280–291CrossRefPubMedGoogle Scholar
  23. Menzel MY, Richmond KL, Dougherty BJ (1985) A chromosome translocation breakpoint map of the Gossypium hirsutum genome. J Hered 76:406–414Google Scholar
  24. Nguyen TB, Giband M, Brottier P, Risterucci AM, Lacape JM (2004) Wide coverage of the tetraploid cotton genome using newly developed microsatellite markers. Theor Appl Genet 109:167–175CrossRefPubMedGoogle Scholar
  25. Percival AE, Wendel JF, Stewart JM (1999) Taxonomy and germplasm resources. In: Smith CW, Cothren JT (eds) Cotton: origin, history, technology, and production. Wiley, New York, pp 33–63Google Scholar
  26. Price HJ, Stelly DM, McKnight TD, Scheuring CF, Raska D, Michaelson MJ, Bergey D (1990) Molecular cytogenetic mapping of a nucleolar organizer region in cotton. J Hered 81:365–370Google Scholar
  27. Reinisch AJ, Dong JM, Brubaker CL, Stelly DM, Wendel JF, Paterson AH (1994) A detailed RFLP map of cotton (Gossypium hirsutum × Gossypium barbadense): chromosome organization and evolution in a disomic polyploid genome. Genetics 138:829–847PubMedGoogle Scholar
  28. Rong JK, Abbey C, Bowers JE, Brubaker CL, Chang C, Chee PW, Delmonte TA, Ding X, Garza JJ, Marler BS, Park C, Pierce GJ, Rainey KM, Rastogi VK, Schulze SR, Trolinder NL, Wendel JF, Wilkins TA, Williams-Coplin TD, Wing RA, Wright RJ, Zhao X, Zhu L, Paterson AH (2004) A 3347-locus genetic recombination map of sequence-tagged sites reveals features of genome organization, transmission and evolution of cotton (Gossypium). Genetics 166:389–417CrossRefPubMedGoogle Scholar
  29. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring HarborGoogle Scholar
  30. Shappley ZW, Jenkins JN, Meredith WR, McCarty JCJ (1998) An RFLP linkage map of Upland cotton, Gossypium hirsutum L. Theor Appl Genet 97:756–761CrossRefGoogle Scholar
  31. Song XL, Wang K, Guo WZ, Zhang J, Zhang TZ (2005) A comparison of genetic maps constructed from haploid and BC mapping populations from the same crossing between Gossypium hirsutum L. and Gossypium barbadense L. Genome 48:378–390CrossRefPubMedGoogle Scholar
  32. Stelly DM (1993) Interfacing cytogenetics with the cotton genome mapping effort. Beltwild Cotton Confence, pp 1545–1550Google Scholar
  33. Ulloa M, Meredith WR (2000) Genetic linkage map and QTL analysis of agronomic and fiber quality traits in an intraspecific population. J Cotton Sci 4:161–170Google Scholar
  34. Ulloa M, Meredith WR, Shappley ZW, Kahler AL (2002) RFLP genetic linkage maps from four F2–3 populations and a joinmap of Gossypium hirsutum L. Theor Appl Genet 104:200–208CrossRefPubMedGoogle Scholar
  35. Wang CY, Wang KB, Wang WK, Li MX, Song GL, Cui RX, Li SH, Zhang XD, Zhang JM (1999) Protocol of cotton FISH of somatic chromosomes with gDNA as probes. Cotton Sci 11:79–83Google Scholar
  36. Wang K, Zhang YJ, Zhang TZ (2005) A high throughput approach for cotton BAC-DNA isolation. Cotton Sci 17:125–126Google Scholar
  37. Wendel JF (1989) New world cottons contain Old World cytoplasm. Proc Natl Acad Sci USA 86:4132–4136PubMedCrossRefGoogle Scholar
  38. Wendel JF, Schnabel A, Seleman T (1995) An unusual ribosomal DNA sequence from Gossypium gossypioides reveals ancient, cryptic, intergenomic introgression. Mol Phylogenet Evol 4:298–313CrossRefPubMedGoogle Scholar
  39. Zhang J, Guo WZ, Zhang TZ (2002) Molecular linkage map of allotetraploid cotton (Gossypium hirsutum L. × Gossypium barbadense L.) with a haploid population. Theor Appl Genet 105:166–1174Google Scholar
  40. Zhao XP, Si Y, Hanson RE, Crane CF, Price HJ, Stelly DM, Wendel JF, Paterson AH (1998) Dispersed repetitive DNA has spread to new genomes since polyploid formation in cotton. Genome Res 5:479–492Google Scholar
  41. Zwick MS, Hanson RE, McKnight TD, Islam-Faridi MN, Stelly DM,Wing RA, Price HJ (1997) A rapid procedure for the isolation of Cot-l DNA from plants. Genome 40:138–142CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Kai Wang
    • 1
  • Xianliang Song
    • 1
  • Zhiguo Han
    • 1
  • Wangzhen Guo
    • 1
  • John Z. Yu
    • 2
  • Jing Sun
    • 1
  • Jiaju Pan
    • 1
  • Russell J. Kohel
    • 2
  • Tianzhen Zhang
    • 1
    Email author
  1. 1.National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Research InstituteNanjing Agricultural UniversityNanjingChina
  2. 2.USDA-ARS, Southern Plains Agricultural Research CenterCrop Germplasm Research UnitCollege StationUSA

Personalised recommendations