Fine mapping Fhb1, a major gene controlling fusarium head blight resistance in bread wheat (Triticum aestivum L.)

  • Patricia A. Cuthbert
  • Daryl J. SomersEmail author
  • Julian Thomas
  • Sylvie Cloutier
  • Anita Brulé-Babel
Original Paper


A major fusarium head blight (FHB) resistance gene Fhb1 (syn. Qfhs.ndsu-3BS) was fine mapped on the distal segment of chromosome 3BS of spring wheat (Triticum aestivum L.) as a Mendelian factor. FHB resistant parents, Sumai 3 and Nyubai, were used as sources of this gene. Two mapping populations were developed to facilitate segregation of Qfhs.ndsu-3BS in either a fixed resistant (Sumai 3*5/Thatcher) (S/T) or fixed susceptible (HC374/3*98B69-L47) (HC/98) genetic background (HC374 = Wuhan1/Nyubai) for Type II resistance. Type II resistance (disease spread within the spike) was phenotyped in the greenhouse using single floret injections with a mixture of macro-conidia of three virulent strains of Fusarium graminearum. Due to the limited heterogeneity in the genetic background of the crosses and based on the spread of infection, fixed recombinants in the interval between molecular markers XGWM533 and XGWM493 on 3BS could be assigned to discrete “resistant” and “susceptible” classes. The phenotypic distribution was bimodal with progeny clearly resembling either the resistant or susceptible parent. Marker order for the two maps was identical with the exception of marker STS-3BS 142, which was not polymorphic in the HC/98 population. The major gene Fhb1 was successfully fine mapped on chromosome 3BS in the same location in the two populations within a 1.27-cM interval (S/T) and a 6.05-cM interval (HC/98). Fine mapping of Fhb1 in wheat provides tightly linked markers that can reduce linkage drag associated with marker-assisted selection of Fhb1 and assist in the isolation, sequencing and functional identification of the underlying resistance gene.


Mapping Population Fusarium Head Blight Fusarium Head Blight Resistance Inoculation Point Recombinant Plant 
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The authors would like to acknowledge the expert assistance of the following persons who made significant contributions to the completion of the present study: Xuiling Zhang, University of Minnesota, for guidance in acquiring specimen bags for greenhouse inoculations; Monika Eng, Allison Brown-Hoeppner, Mira Popovic, Zlatko Popovic, and Yanfen Zheng for assistance in collecting a portion of the genotypic data; Ron Kaethler, Kevin Morgan, and Kirsten Slusarenko for preparation of F. graminearum inoculum for phenotyping.


  1. Andersen AL (1948) The development of Gibberella zeae head blight of wheat. Phytopathology 38:595–611Google Scholar
  2. Anderson JA, Stack RW, Liu S, Waldron BL, Fjeld AD, Coyne C, Moreno-Sevilla B, Mitchell-Fetch J, Song QJ, Cregan PB, Frohberg RC (2001) DNA markers for Fusarium head blight resistance QTLs in two wheat populations. Theor Appl Genet 102:1164–1168CrossRefGoogle Scholar
  3. Bai G, Shaner G (1994) Scab of wheat: prospects for control. Plant Dis 78:760–766Google Scholar
  4. Bai G, Xiao QP (1989) Genetic analysis on resistance to scab in six wheat cultivars. Acta Agric Shanghai 5:17–23Google Scholar
  5. Bai G, Zhou CF, Qian CM, Ge YF (1990) Genetic analysis on resistance genes to scab spread in wheat cultivars. In: Zhu LH (ed) Advances in genetic researches on resistance to diseases in major crops. Jiangsu Sci Tech Publ House, Nanjing, China, pp. 171–177Google Scholar
  6. Bai G, Kolb FL, Shaner G, Domier LL (1999) Amplified fragment length polymorphism markers linked to a major quantitative trait locus controlling scab resistance in wheat. Phytopathology 89:343–348CrossRefPubMedGoogle Scholar
  7. Ban T (2000) Review studies on the genetics of resistance to Fusarium head blight caused by Fusarium graminearum in wheat. In: Raupp J, Ma Z, Chen P, Liu D (eds) Proceedings of the international symposium wheat improve scab resistance, 5–11 May 2000, Suzhou and Nanjing, China, pp 82–93Google Scholar
  8. Buerstmayr H, Lemmens M, Hartl L, Doldi L, Steiner B, Stierschneider M, Ruckenbauer P (2002) Molecular mapping of QTLs for Fusarium head blight resistance in spring wheat. I. Resistance to fungal spread (Type II resistance). Theor Appl Genet 104:84–91PubMedCrossRefGoogle Scholar
  9. Faris JD, Haen KM, Gill BS (2000) Saturation mapping of a gene-rich recombination hot spot region in wheat. Genetics 154:823–835PubMedGoogle Scholar
  10. Gilbert J, Tekauz A (2000) Review: recent developments in research on fusarium head blight of wheat in Canada. Can J Plant Pathol 22:1–8Google Scholar
  11. Gill KS, Gill BS, Endo TR, Taylor T (1996) Identification and high-density mapping of gene-rich regions in chromosome group I of wheat. Genetics 144:1883–1891PubMedGoogle Scholar
  12. Gu JQ (1983) A study on genetics of wheat scab resistance. Scientia Agric Sin 6:61–64Google Scholar
  13. Han FP, Fedak G, Ouellet T, Dan H, Somers DJ (2005) Mapping of genes expressed in Fusarium graminearum-infected heads of wheat cultivar ‘Frontana’. Genome 48:88–96PubMedCrossRefGoogle Scholar
  14. Hanson EW, Ausemus ER, Stakman EC (1950) Varietal resistance of spring wheats to fusarial head blight. Phytopathology 40:902–914Google Scholar
  15. Liu S, Anderson JA (2003) Targeted molecular mapping of a major wheat QTL for Fusarium head blight using ESTs and synteny with rice. Genome 46:817–823PubMedCrossRefGoogle Scholar
  16. Liu S, Pumphrey M, Anderson JA (2003) Fine mapping a major wheat QTL for Fusarium head blight resistance. In: 10th international proceeding on the wheat genetics symposium, Vol. 1, Section 6, Paestum, Italy, 1–6 September 2003, pp 368–371Google Scholar
  17. Mesterhazy A (1995) Types and components of resistance to Fusarium head blight of wheat. Plant Breed 114:377–386CrossRefGoogle Scholar
  18. Röder MS, Korzun V, Wandehake K, Planscheke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023PubMedGoogle Scholar
  19. Schroeder HW, Christensen JJ (1963) Factors affecting resistance of wheat to scab by Gibberella zeae. Phytopathology 53:831–838Google Scholar
  20. Schuelke M (2000) An economic method for the fluorescent labelling of PCR fragments. Nat Biotechnol 18:233–234PubMedCrossRefGoogle Scholar
  21. Scott IT (1927) Varietal resistance and susceptibility to wheat scab. Missouri Agr Exp Stn Res Bull 111:14Google Scholar
  22. Somers DJ, Fedak G, Savard M (2003) Molecular mapping of novel genes controlling Fusarium head blight resistance and deoxynivalenol accumulation in spring wheat. Genome 46:555–564PubMedCrossRefGoogle Scholar
  23. Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114PubMedCrossRefGoogle Scholar
  24. Steiner B, Lemmens M, Griesser M, Scholz U, Schondelmaier J, Buerstmayr H (2004) Molecular mapping of resistance to Fusarium head blight in the spring wheat cultivar Frontana. Theor Appl Genet 109:215–224PubMedCrossRefGoogle Scholar
  25. Sung JM, Cook RJ (1981) Effect of water potential on reproduction and spore germination by Fusarium roseum ‘Graminearum’, ‘Culmorum’ and ‘Avenaceum’. Phytopathology 71:499–504CrossRefGoogle Scholar
  26. Waldron BL, Moreno-Sevilla B, Anderson JA, Stack RW, Frohberg RC (1999) RFLP mapping of QTL for Fusarium head blight resistance in wheat. Crop Sci 39:805–811Google Scholar
  27. Yang ZP, Gilbert J, Somers DJ, Fedak G, Procunier JD, McKenzie R (2003) Marker-assisted selection of fusarium head blight resistance genes in two doubled-haploid populations of wheat. Mol Breed 12:309–317CrossRefGoogle Scholar
  28. Yang Z, Gilbert J, Fedak G, Somers DJ (2005) Genetic characterization of QTL associated with resistance to Fusarium head blight in a doubled-haploid spring wheat population. Genome 48:187–196PubMedGoogle Scholar
  29. Zhou WC, Kolb FL, Bai G, Shaner G, Domier LL (2002) Genetic analysis of scab resistance QTL in wheat with microsatellite and AFLP markers. Genome 45:719–727PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Patricia A. Cuthbert
    • 1
  • Daryl J. Somers
    • 2
    Email author
  • Julian Thomas
    • 2
  • Sylvie Cloutier
    • 2
  • Anita Brulé-Babel
    • 1
  1. 1.Department of Plant ScienceUniversity of ManitobaWinnipegCanada
  2. 2.Agriculture and Agri-Food CanadaCereal Research CentreWinnipegCanada

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