An enhanced microsatellite map of diploid Fragaria
- 476 Downloads
A total of 45 microsatellites (SSRs) were developed for mapping in Fragaria. They included 31 newly isolated codominant genomic SSRs from F. nubicola and a further 14 SSRs, derived from an expressed sequence tagged library (EST-SSRs) of the cultivated strawberry, F. × ananassa. These, and an additional 64 previously characterised but unmapped SSRs and EST-SSRs, were scored in the diploid Fragaria interspecific F2 mapping population (FV×FN) derived from a cross between F. vesca 815 and F. nubicola 601. The cosegregation data of these 109 SSRs, and of 73 previously mapped molecular markers, were used to elaborate an enhanced linkage map. The map is composed of 182 molecular markers (175 microsatellites, six gene specific markers and one sequence-characterised amplified region) and spans 424 cM over seven linkage groups. The average marker spacing is 2.3 cM/marker and the map now contains just eight gaps longer than 10 cM. The transferability of the new SSR markers to the cultivated strawberry was demonstrated using eight cultivars. Because of the transferable nature of these markers, the map produced will provide a useful reference framework for the development of linkage maps of the cultivated strawberry and for the development of other key resources for Fragaria such as a physical map. In addition, the map now provides a framework upon which to place transferable markers, such as genes of known function, for comparative mapping purposes within Rosaceae.
KeywordsFragaria Genetic mapping Microsatellites EST Functional genomics
This research was supported at EMR by funds from Defra, the University of Reading Research Endowment Trust Fund, the East Malling Trust for Horticultural Research and the Worshipful Company of Fruiterers. Funding at IRTA was provided by grant no. AGL2003-04691 from CICYT (Spain), and a fellowship to S. Vilanova from MEC of Spain (EX2003-1083). Funding at INRA was provided by Région Aquitaine and the European Community (FEDER funds). The authors also to acknowledge the North American Strawberry Growers’ Association for funding allocated toward the sequencing of ESTs used in this study at the University of Florida.
- Davis TM, Yu H (1997) A linkage map of the diploid strawberry, Fragaria vesca. J Hered 88:215–221Google Scholar
- Lewers KS, Styan SMN, Hokanson SC, Bassil NV (2005) Strawberry GenBank-derived and genomic simple sequence repeat (SSR) markers and their utility with strawberry, blackberry, and red and black raspberry. J Am Soc Hortic Sci 130:102–115Google Scholar
- Monfort A, Vilanova S, Davis TM, Arús P (2005) A new set of polymorphic simple sequence repeat (SSR) markers from a wild strawberry (Fragaria vesca) are transferable to other diploid Fragaria species and to Fragaria × ananassa. Mol Ecol Notes; doi:10.1111/j.1471–8286.2005.01191.xGoogle Scholar
- Rozen S, Skaletsky HJ (1998) PRIMER 3. Code available at http://www.genome.wi.mit.edu/genome_software/other/primer3.html
- Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring HarborGoogle Scholar
- Van Ooijen JW, Voorrips RE (2001) JoinMap 3.0: Software for the calculation of genetic linkage maps. Plant Research International, WageningenGoogle Scholar
- Yamamoto Y, Sato E, Shimizu T, Nakamich N, Sato S, Kato T, Tabata S, Nagatani A, Yamashino T, Mizuno T (2003) Comparative genetic studies on the APRR5 and APRR7 genes belonging to the APRR1/TOC1 quintet implicated in circadian rhythm, control of flowering time, and early photomorphogenesis. Plant Cell Physiol 44:1119–1130CrossRefPubMedGoogle Scholar