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Theoretical and Applied Genetics

, Volume 111, Issue 4, pp 711–722 | Cite as

Genetic modification of potato against microbial diseases: in vitro and in planta activity of a dermaseptin B1 derivative, MsrA2

  • Milan Osusky
  • Lubica Osuska
  • William Kay
  • Santosh Misra
Original Paper

Abstract

Dermaseptin B1 is a potent cationic antimicrobial peptide found in skin secretions of the arboreal frog Phyllomedusa bicolor. A synthetic derivative of dermaseptin B1, MsrA2 (N-Met-dermaseptin B1), elicited strong antimicrobial activities against various phytopathogenic fungi and bacteria in vitro. To assess its potential for plant protection, MsrA2 was expressed at low levels (1–5 μg/g of fresh tissue) in the transgenic potato (Solanum tuberosum L.) cv. Desiree. Stringent challenges of these transgenic potato plants with a variety of highly virulent fungal phytopathogens—Alternaria, Cercospora, Fusarium, Phytophthora, Pythium, Rhizoctonia and Verticillium species—and with the bacterial pathogen Erwinia carotovora demonstrated that the plants had an unusually broad-spectrum and powerful resistance to infection. MsrA2 profoundly protected both plants and tubers from diseases such as late blight, dry rot and pink rot and markedly extended the storage life of tubers. Due to these properties in planta, MsrA2 is proposed as an ideal antimicrobial peptide candidate to significantly increase resistance to phytopathogens and improve quality in a variety of crops worldwide with the potential to obviate fungicides and facilitate storage under difficult conditions.

Keywords

Transgenic Plant Late Blight Phytophthora Transgenic Potato Transgenic Potato Plant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

We thank X. Yu, B. Forward, T. Stevenson and B. Allen for expert technical assistance, Dr. Zamir Punja (Simon Fraser University, Burnaby, B.C., Canada) and Dr. Harold Platt (Agriculture & Agri-Food Canada) for providing fungal pathogens. This work was funded by a grant to SM from the Canadian Bacterial Diseases Network.

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Milan Osusky
    • 1
  • Lubica Osuska
    • 1
  • William Kay
    • 1
  • Santosh Misra
    • 1
  1. 1.Department of Biochemistry and MicrobiologyUniversity of VictoriaVictoriaCanada

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