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Theoretical and Applied Genetics

, Volume 111, Issue 4, pp 696–701 | Cite as

Molecular mapping of the fertility restorer gene for ms-CW-type cytoplasmic male sterility of rice

  • Sota Fujii
  • Kinya ToriyamaEmail author
Original Paper

Abstract

Cytoplasmic male sterility (CMS) of rice (Oryza sativa L.) was first reported using the cytoplasm of a Chinese wild rice, Oryza rufipogon Griff. strain W1. However, it was not possible to characterize this ms-CW-type CMS in more detail until a restorer line had been developed due to the lack of restorer genes among cultivars thus far tested. The breeding of a restorer line (W1-R) was eventually achieved by transferring the restorer gene(s) of W1 to a cultivar. We report here the characterization of the ms-CW pollen grains and mapping of the restorer gene for ms-CW-type CMS. Pollen grains of the male-sterile plants appeared to be normal and viable based on the fluorochromatic reaction test, but they did not germinate on normal stigmas. The 1:1 segregation of fertile and sterile plants in a BC1F1 population from a cross between W1-R and a maintainer line demonstrated that fertility restoration is controlled by a single gene. The fertile seed set of all the F2 plants examined indicated that the fertility restoration functions gametophytically. We designated the fertility restorer gene Rfcw. Using cleaved amplified polymorphic sequence (CAPS) and simple sequence repeat (SSR) markers, we localized Rfcw to chromosome 4 with a genetic distance of 0.6 cM from the nearest SSR marker.

Keywords

Simple Sequence Repeat Marker Cytoplasmic Male Sterility Cleave Amplify Polymorphic Sequence Fertility Restoration Restorer Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This study was partially supported by a grant for “Functional analysis of genes relevant to agriculturally important traits in rice genome” from the Ministry of Agriculture, Forestry and Fisheries, Japan, and by a Grant-in-Aid from the Ministry of Education, Science and Culture, Japan.

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  1. 1.Laboratory of Environmental Biotechnology, Graduate School of Agricultural ScienceTohoku UniversitySendaiJapan

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