Theoretical and Applied Genetics

, Volume 111, Issue 3, pp 530–539 | Cite as

Patterns of AFLP variation in a core subset of cultivated hexaploid oat germplasm

  • Yong Bi Fu
  • Gregory W. Peterson
  • David Williams
  • Ken W. Richards
  • Jennifer Mitchell Fetch
Original Paper

Abstract

Many core collections have been developed from large collections of crop germplasm, but most of these have not been characterized, particularly using molecular techniques, for germplasm management and utilization. We have attempted to characterize a structured sample representing a world collection of 11,622 cultivated hexaploid oat accessions in the hope of understanding the genetic structure of the world collection. The amplified fragment length polymorphism (AFLP) technique was applied to screen 670 accessions representing 79 countries and one group of uncertain origin. For each accession, 170 AFLP polymorphic bands detected by five AFLP primer pairs were scored. Analyses of the AFLP data showed the effectiveness of the stratified sampling applied in capturing country-wise AFLP variation. The frequencies of polymorphic bands ranged from 0.11 to 0.99, with an average of 0.72. The majority (89.9%) of the AFLP variation resided within accessions of each country, and only 6.2% of the AFLP differences existed among accessions of major geographic regions. Accessions from the Mediterranean region were the most distinct, while those from Russia and the USA were the most diverse. The two distinct groups that were observed were separated largely on the basis of common oat and red oat. Red oat was genetically more diverse than its common and hull-less counterparts, and hull-less oat was more related to common oat than red oat. Landrace and non-landrace accessions displayed similar AFLP variation patterns. These patterns are significant for understanding the domestication of cultivated oat and are useful in classifying the intraspecific diversity of oat germplasm, developing specific core subsets of the oat collection, and exploring new sources of genes for oat improvement.

Notes

Acknowledgements

The authors would like to thank Drs. Axel Diederichsen, James Chong, Tom Fetch, and Brian Rossnagel for their stimulating discussions on the project; Mr. Dallas Kessler and Ms. Angela Taylor for their technical assistance in sampling, planting, and screening the germplasm; and Drs. Van Ripley and Axel Diederichsen for their helpful comments on the manuscript.

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Yong Bi Fu
    • 1
  • Gregory W. Peterson
    • 1
  • David Williams
    • 1
  • Ken W. Richards
    • 1
  • Jennifer Mitchell Fetch
    • 2
  1. 1.Plant Gene Resources of Canada, Saskatoon Research CentreAgriculture and Agri-Food CanadaSaskatoonCanada
  2. 2.Cereal Research CentreAgriculture and Agri-Food CanadaWinnipegCanada

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