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Theoretical and Applied Genetics

, Volume 111, Issue 2, pp 226–232 | Cite as

Segregation patterns of isozyme loci and microsatellite markers show the diploidy of African yam Dioscorea rotundata (2n=40)

  • Nora Scarcelli
  • Ogoubi Daïnou
  • Clément Agbangla
  • Serge Tostain
  • Jean-Louis Pham
Original Paper

Abstract

The cultivated yam species Dioscorea rotundata (2n=40) has been considered by most authors as a tetraploid species with a basic chromosome number of ten. In this paper, we analysed the segregation of two isozyme loci and six microsatellite markers in the progeny of a self-fertilised monoecious plant. For the eight markers, segregation patterns could be explained by only two genetic models: diploidy or tetraploidy with two null alleles. Given the nature of studied markers, the most parsimonious hypothesis was that the parental plant was diploid. These results, data from a diversity survey and results of other authors led to the conclusion that D. rotundata is a diploid species.

Keywords

Yam Dioscorea rotundata Ploidy level Microsatellite Isozyme Segregation 

Notes

Acknowledgements

We thank Jacques David, Serge Hamon and Joelle Ronfort for their comments on earlier drafts of the manuscript.

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Nora Scarcelli
    • 1
  • Ogoubi Daïnou
    • 2
  • Clément Agbangla
    • 2
  • Serge Tostain
    • 1
  • Jean-Louis Pham
    • 1
  1. 1.Equipe DYNADIV, UMR 1097 Diversité et Génomes des Plantes CultivéesInstitut de Recherche pour le Développement (IRD)Montpellier cedex 5France
  2. 2.Laboratoire de GénétiqueFAST-Université d’Abomey-CalaviCotonou, Bénin

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