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Theoretical and Applied Genetics

, Volume 110, Issue 8, pp 1458–1466 | Cite as

The Vrn-H2 locus is a major determinant of flowering time in a facultative × winter growth habit barley (Hordeum vulgare L.) mapping population

  • I. KarsaiEmail author
  • P. Szűcs
  • K. Mészáros
  • T. Filichkina
  • P. M. Hayes
  • J. S. Skinner
  • L. Láng
  • Z. Bedő
Original Paper

Abstract

With the aim of dissecting the genetic determinants of flowering time, vernalization response, and photoperiod sensitivity, we mapped the candidate genes for Vrn-H2 and Vrn-H1 in a facultative × winter barley mapping population and determined their relationships with flowering time and vernalization via QTL analysis. The Vrn-H2 candidate ZCCT-H genes were completely missing from the facultative parent and present in the winter barley parent. This gene was the major determinant of flowering time under long photoperiods in controlled environment experiments, irrespective of vernalization, and under spring-sown field experiments. It was the sole determinant of vernalization response, but the effect of the deletion was modulated by photoperiods when the vernalization requirement was fulfilled. There was no effect under short photoperiods. The Vrn-H1 candidate gene (HvBM5A) was mapped based on a microsatellite polymorphism we identified in the promoter of this gene. Otherwise, the HvBM5A alleles for the two parents were identical. Therefore, the significant flowering time QTL effect associated with this locus suggests tight linkage rather than pleiotropy. This QTL effect was smaller in magnitude than those associated with the Vrn-H2 locus and was significant in two-way interactions with Vrn-H2. The Vrn-H1 locus had no effect on vernalization response. Our results support the Vrn-H2/Vrn-H1 repressor/structural gene model for vernalization response in barley and suggest that photoperiod may also affect the Vrn genes or tightly linked loci.

Keywords

Flowering Time Doubled Haploid Doubled Haploid Line Short Photoperiod Photoperiod Sensitivity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This project was funded by the Hungarian National Research and Innovation Fund (NKFP) research grant no. 4/0020/2002 and the United States National Science Foundation project no. DBIO 110124.

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • I. Karsai
    • 1
    Email author
  • P. Szűcs
    • 1
  • K. Mészáros
    • 1
  • T. Filichkina
    • 2
  • P. M. Hayes
    • 2
  • J. S. Skinner
    • 2
  • L. Láng
    • 1
  • Z. Bedő
    • 1
  1. 1.Hungarian Academy of SciencesAgricultural Research InstituteMartonvásárHungary
  2. 2.Department of Crop and Soil ScienceOregon State UniversityCorvallisUSA

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