Advertisement

Theoretical and Applied Genetics

, Volume 110, Issue 7, pp 1218–1225 | Cite as

DNA markers associated with low Fusarium head blight incidence and narrow flower opening in wheat

  • J. Gilsinger
  • L. Kong
  • X. Shen
  • H. OhmEmail author
Original Paper

Abstract

Fusarium head blight (FHB) of wheat, caused by Fusarium graminearum, is an important fungal disease in many wheat-growing areas of the world. The objectives of this study were to determine the relationship between width and duration of flower opening and incidence of FHB in wheat, and to identify DNA markers associated with narrow flower opening and low FHB incidence. It was hypothesized that wheat lines whose flowers open briefly and narrowly have a reduced risk of infection. To test the hypothesis, we crossed wheat cultivars Patterson and Goldfield to generate a population of 100 random F2-derived recombinant inbred lines (RILs). Florets of Patterson open wide; florets of Goldfield tend to stay closed. The population of RILs was characterized for FHB incidence and flower opening width (FOW) and duration in the F7:9 and F7:10 generations. Of the 305 simple sequence repeat primer pairs screened on the parents, 79 amplified polymorphic DNA bands. Pooled DNA from each of the two bulks was tested with these 79 SSR primer pairs. Four markers were found to have significant marker-trait association with low FHB incidence and narrow flower opening. The major QTL effect associated with narrow flower opening and low FHB incidence was found between the map interval Xbarc200Xgwm210, explaining 29% of the phenotypic variation for FHB incidence averaged over six replicated tests in Indiana in 2002 and 2003. This adds credence to the hypothesis that narrow flower opening is responsible for low FHB incidence in this population. Breeding wheat lines for both morphological avoidance, such as narrow flower opening, and physiological resistance to FHB may be valuable in future breeding research to reduce crop production and grain quality losses in wheat due to FHB.

Keywords

Recombinant Inbred Line Fusarium Head Blight Flower Opening Fusarium Head Blight Resistance Fusarium Head Blight Severity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

The authors acknowledge the financial support from USDA-ARS, USWBSI competitive grant no. 59-0790-9-057, and Purdue University.

References

  1. Bai G, Shaner G (1994) Scab of wheat: prospects for control. Plant Dis 78:760–765Google Scholar
  2. Chhabra AK, Sethi SK (1991) Inheritance of cleistogamous flowering in durum wheat (Triticum durum). Euphytica 55:147–150Google Scholar
  3. Doerge RW, Churchill GA (1996) Permutation tests for multiple loci affecting a quantitative character. Genetics 142:285–294PubMedGoogle Scholar
  4. Kolb FL, Bai GH, Muehlbauer GJ, Anderson JA, Smith KP, Fedak G (2001) Host plant resistance genes for Fusarium head blight: mapping and manipulation with molecular markers. Crop Sci 41:611–619Google Scholar
  5. McMullen M, Jones R, Gallenberg D (1997) Scab of wheat and barley: a re-emerging disease of devastating impact. Plant Dis 81:12Google Scholar
  6. Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832PubMedGoogle Scholar
  7. Ohm HW, Shaner GE, Ratcliffe RH, Huber DM, Buechley GC, Cambron SE (1998) Registration of ‘Patterson’ wheat. Crop Sci 38:553Google Scholar
  8. Ohm HW, Shaner GE, Ratcliffe RH, Huber DM, Sharma H, Perry KL, Buechley GC, Cambron SE (2000) Registration of ‘Goldfield’ wheat. Crop Sci 40:581–582Google Scholar
  9. Percival J (1921) The wheat plant. Duckworth, LondonGoogle Scholar
  10. Pestsova E, Ganal MW, Röder MS (2000) Isolation and mapping of microsatellite markers specific for the D genome of bread wheat. Genome 43:689–697CrossRefPubMedGoogle Scholar
  11. Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023PubMedGoogle Scholar
  12. Rudd JC, Horsley RD, McKendry AL, Elias EM (2001) Host plant resistance genes for Fusarium head blight: I. Sources, mechanisms, and utility in conventional breeding systems. Crop Sci 41:620–627Google Scholar
  13. Saghai-Maroof MA, Soliman K, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mmendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 81:8014–8018PubMedGoogle Scholar
  14. Schroder HW, Christensen JJ (1963) Factors affecting resistance of wheat to scab caused by Gibberella zeae. Phytopathology 53:831–838Google Scholar
  15. Snijders CHA (1990) Genetic variation for resistance to Fusarium head blight in bread wheat. Euphytica 50:171–179Google Scholar
  16. Steiner B, Lemmens M, Griesser M, Scholz U, Schondelmaier J, Buerstmayr H (2004) Molecular mapping of resistance to Fusarium head blight in the spring wheat cultivar Frontana. Theor Appl Genet 109:215–224CrossRefPubMedGoogle Scholar
  17. Ward R, Cregan P, Song Q, Shi J, Gill B, Singh S (2003) 544 BARC SSRs from Rick Ward. http://wheat.pw.usda.gov/ggpages/whatsnew/2003.shtml
  18. Wiese MV (1987) Compendium of wheat diseases, 2nd edn. APS Press, St. PaulGoogle Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  1. 1.Department of AgronomyPurdue UniversityWest LafayetteUSA

Personalised recommendations