Theoretical and Applied Genetics

, Volume 112, Issue 5, pp 913–923

Molecular mapping of genes for resistance to the bean pod weevil (Apion godmani Wagner) in common bean

Original Paper

Abstract

The bean pod weevil (Apion godmani Wagner) is a serious insect pest of common beans (Phaseolus vulgaris L.) grown in Mexico and Central America that is best controlled by host-plant resistance available in Durango or Jalisco genotypes such as J-117. Given unreliable infestation by the insect, the use of marker-assisted selection is desirable. In the present study, we developed a set of nine molecular markers for Apion resistance and mapped them to loci on chromosomes 2, 3, 4 and 6 (linkage groups b01, b08, b07and b11, respectively) based on genetic analysis of an F5:10 susceptible × resistant recombinant inbred line population (Jamapa × J-117) and two reference mapping populations (DOR364 × G19833 and BAT93 × JaloEEP558) for which chromosome and linkage group designations are known. All the markers were derived from randomly amplified polymorphic DNA (RAPD) bands that were identified through bulked segregant analysis and cloned for conversion to sequence tagged site (STS) markers. One of the markers was dominant while four detected polymorphism upon digestion with restriction enzymes. The other markers were mapped as RAPD fragments. Phenotypic data for the population was based on the evaluation of percentage seed damage in replicated trials conducted over four seasons in Mexico. In single point regression analysis, individual markers explained from 3.5 to 22.5% of the variance for the resistance trait with the most significant markers overall being F10-500S, U1-1400R, R20-1200S, W9-1300S and Z4-800S, all markers that mapped to chromosome 2 (b01). Two additional significant markers, B1-1400R and W6-800R, were mapped to chromosome 6 (b11) and explained from 4.3 to 10.2% of variance depending on the season. The latter of these markers was a dominant STS marker that may find immediate utility in marker-assisted selection. The association of these two loci with the Agr and Agm genes is discussed as well as the possibility of additional resistance genes on chromosome 4 (b07) and chromosome 3 (b08). These are among the first specific markers developed for tagging insect resistance in common bean and are expected to be useful for evaluating the mechanism of resistance to A. godmani.

Keywords

Insect resistance genes Seed coat peroxidase Hypersensitive response 

References

  1. Afanador LK, Hadley SD, Kelly JD (1998) Adoption of a mini-prep DNA extraction method for RAPD marker analysis in common bean (Phaseolus vulgaris L). Annu Rep Bean Improv Coop 36:10–11Google Scholar
  2. Beebe S, Cardona C, Díaz O, Rodríguez F, Mancia E, Ajquejay S (1993) Development of common bean (Phaseolus vulgaris L.) lines resistant to the bean pod weevil, Apion godmani Wagner, in Central America. Euphytica 96:83–88CrossRefGoogle Scholar
  3. Biradar SK, Sundaram RM, Thirumurugan T, Bentur JS, Amudhan S, Shenoy VV, Mishra B, Bennett J, Sharma NP (2004) Identification of flanking SSR markers for a major rice gall midge resistance gene GM1 and their validation. Theor Appl Genet 109:1468–1473CrossRefPubMedGoogle Scholar
  4. Blair MW, Pedraza F, Buendia HF, Gaitán-Solís E, Beebe SE, Gepts P, Tohme J (2003) Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.). Theor Appl Genet 107:1362–1374CrossRefPubMedGoogle Scholar
  5. Bolwell GP, Bindschedler LV, Blee KA, Butt VS, Davies DR, Gardner SL, Gerrish C, Minibayeva F (2002) The apoplastic oxidative burst in response to biotic stress in plants: a three-component system. J Exp Bot 53:1367–1376CrossRefPubMedGoogle Scholar
  6. Cardona C (1989) Insects and other invertebrate bean pests in Latin America, pp 505–570. In: Schwartz HF, Pastor-Corrales A (eds) Bean production problems in the tropics, 2nd edn. Centro Internacional de Agricultura Tropical, CIAT, CaliGoogle Scholar
  7. Cardona C, Kornegay J (1999) Bean germplasm for insect resistance. In: Clement SL, Quisenberry SS (eds) Global plant genetic resources for insect-resistant crops. CRC Press, BostonGoogle Scholar
  8. Dwiekat I, Ohm H, Patterson F, Cambron S (1997) Identification of RAPD markers for 11 Hessian fly resistance genes in wheat. Theor Appl Genet 94:419–423CrossRefGoogle Scholar
  9. Fernandes GW (1990) Hypersensitivity: a neglected plant resistance mechanism against insect herbivores. Environ Entomol 19:1173–1182Google Scholar
  10. Frei A, Blair MW, Cardona C, Beebe SE, Gu H, Dorn S (2005) Identification of quantitative trait loci for resistance to Thrips palmi Karny in Common Bean (Phaseolus vulgaris L.). Crop Sci 45:379–387CrossRefGoogle Scholar
  11. Freyre R, Skroch PW, Geffory V, Adam-Blondon AF, Shirmohamadali A, Johnson WC, Llaca V, Nodari RO, Periera PA, Tsai SM, Tohme J, Dron M, Nienhuis J, Vallejos CE, Gepts P (1998) Towards an integrated linkage map of common bean. 4. Development of a core linkage map and alignment of RFLP maps. Theor Appl Genet 97:847–856CrossRefGoogle Scholar
  12. Garza R, Muruaga JS (1993) Resistencia al ataque del picudo del ejote Apion spp. In: Frijol Phaseolus spp. Agron Mesoam 4:77–80Google Scholar
  13. Garza R, Cardona C, Singh SP (1996) Inheritance of resistance to the bean-pod weevil (Apion godmani Wagner) in common beans from Mexico. Theor Appl Genet 92:357–362CrossRefGoogle Scholar
  14. Garza R, Vera J, Cardona C, Barcenas N, Singh SP (2001) Hypersensitive response of beans to Apion godmani (Coleoptera: Curculionidae). J Econ Entomol 94:958–962PubMedCrossRefGoogle Scholar
  15. Grover PB Jr (1995) Hypersensitive response of wheat to the Hessian fly. Entomol Exp Appl 74:283–294CrossRefGoogle Scholar
  16. Hammond-Kosack KE, Jones JDG (1997) Plant disease resistance genes. Annu Rev Plant Physiol Plant Mol Biol 48:575–607CrossRefPubMedGoogle Scholar
  17. Kaloshian I (2004) Gene-for-gene disease resistance: bridging insect pest and pathogen defense. J Chem Ecol 30:2419–2438CrossRefPubMedGoogle Scholar
  18. Katiyar SK, Tan Y, Huang B, Chandel B, Xu Y, Zhang Y, Xie Z, Bennett J (2001) Molecular mapping of gene GM6(t) which confers resistance against four biotypes of Asian rice gall midge in China. Theor Appl Genet 103:953–961CrossRefGoogle Scholar
  19. Kelly JD, Miklas PN (1998) The role of RAPD markers in breeding for disease resistance in common bean. Mol Breed 4:1–11CrossRefGoogle Scholar
  20. Kelly JD, Gepts P, Miklas PN, Coyne DP (2003) Tagging and mapping of genes and QTL and molecular marker-assisted selection for traits of economic importance in bean and cowpea. Field Crops Res 82:135–154CrossRefGoogle Scholar
  21. Kornegay J, Cardona C (1991) Breeding for insect resistance in beans. In: van Schoonhoven A, Voysest O (eds) Common beans: research for crop improvement, pp 619–648 Google Scholar
  22. Lamb C, Dixon RA (1997) The oxidative burst in plant disease resistance. Annu Rev Plant Physiol Plant Mol Biol 48:251–275CrossRefPubMedGoogle Scholar
  23. Lander ES, Green P, Abrahamson J, Barlow A, Daly M, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181CrossRefPubMedGoogle Scholar
  24. Liu XM, Smith CM, Gill BS, Tolmay B (2001) Microsatellite markers linked to six Russian wheat aphid resistance in wheat. Theor Appl Genet 102:504–510CrossRefGoogle Scholar
  25. Liu XM, Smith CM, Gill BS (2002) Identification of microsatellite markers linked to Russian wheat aphid resistance genes Dn4 and Dn6. Theor Appl Genet 104:1042–1048CrossRefPubMedGoogle Scholar
  26. Llaca V, Gepts P (1996) Pulsed-field gel electrophoresis analysis of the phaseolin locus region in Phaseolus vulgaris. Genome 39:722–729CrossRefPubMedGoogle Scholar
  27. López CE, Acosta IF, Jara C, Pedraza F, Gaitán-Solís E, Gallego G, Beebe S, Tohme J (2003) Identifying resistance gene analogs associated with resistances to different pathogens in common bean. Phytopathology 93:88–95CrossRefPubMedGoogle Scholar
  28. Moore JP, Paul ND, Whittaker JB, Taylor JE (2003) Exogenous jasmonic acid mimics herbivore-induced systemic increases in cell wall bound peroxidase activity and reduction in leaf expansion. Funct Ecol 17:549–554CrossRefGoogle Scholar
  29. Nelson JC (1997) QGENE: software for marker-based genomic analysis and breeding. Mol Breed 3:229–235CrossRefGoogle Scholar
  30. Osborn TC, Blake T, Gepts P, Bliss F (1986) Bean Arcelin. Part 2. Genetic variation, inheritance and linkage relationships of a novel seed protein of Phaseolus vulgaris L. Theor Appl Genet 71:847–855CrossRefGoogle Scholar
  31. Pedrosa A, Vallejos CE, Bachmair A, Schwizer D(2003) Integration of common bean (Phaseolus vulgaris) linkage and chromosomal maps. Theor Appl Genet 106:205–212PubMedGoogle Scholar
  32. Rivkin MI, Vallejos CE, McClean PE (1999) Disease-resistance related sequences in common bean. Genome 42:41–47CrossRefPubMedGoogle Scholar
  33. Sifuentes AJA (1981) Plagas del frijol en México. SARH, INIA. México, D.F. Folleto Técnico No. 78. 28 pGoogle Scholar
  34. Voysest OV (2000) Mejoramiento genético de frijol (Phaseolus vulgaris L.): legado de variedades de América Latina 1930–1999. CIAT publication no. 321, 195 pGoogle Scholar
  35. Yencho GC, Cohen MB, Byrne PF (2000) Applications of tagging and mapping insect resistance loci in plants. Annu Rev Entomol 45:393–422CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  1. 1.CIAT - International Center for Tropical AgricultureMedleyUSA
  2. 2.CIAT - International Center for Tropical AgricultureCaliColombia
  3. 3.Entomology ProgramINIFAPChapingoMexico

Personalised recommendations