Theoretical and Applied Genetics

, Volume 112, Issue 3, pp 562–569

Stacking quantitative trait loci (QTL) for Fusarium head blight resistance from non-adapted sources in an European elite spring wheat background and assessing their effects on deoxynivalenol (DON) content and disease severity

  • T. Miedaner
  • F. Wilde
  • B. Steiner
  • H. Buerstmayr
  • V. Korzun
  • E. Ebmeyer
Original Paper

Abstract

Fusarium head blight (FHB) is a devastating disease in wheat that reduces grain yield, grain quality and contaminates the harvest with deoxynivalenol (DON). As potent resistance sources Sumai 3 and its descendants from China and Frontana from Brazil had been analysed by quantitative trait loci (QTL) mapping. We introgressed and stacked two donor QTL from CM82036 (Sumai 3/Thornbird) located on chromosomes 3B and 5A and one donor QTL from Frontana on chromosome 3A in elite European spring wheat and estimated the effects of the three individual donor QTL and their four combinations on DON, Fusarium exoantigen content, and FHB rating adjusted to heading date. One class with the susceptible QTL alleles served as control. Each of the eight QTL classes was represented by 12–15 F3-derived lines tested in F5 generation as bulked progeny possessing the respective marker alleles homozygously. Traits were evaluated in a field experiment across four locations with spray inoculation of Fusarium culmorum. All three individual donor-QTL alleles significantly reduced DON content and FHB severity compared to the marker class with no donor QTL. The only exception was the donor-QTL allele 3A that had a low, but non-significant effect on FHB severity. The highest effect had the stacked donor-QTL alleles 3B and 5A for both traits. They jointly reduced DON content by 78% and FHB rating by 55% compared to the susceptible QTL class. Analysis of Fusarium exoantigen content illustrates that lower disease severity is associated with less mycelium content in the grain. In conclusion, QTL from non-adapted sources could be verified in a genetic background of German elite spring wheat. Within the QTL classes significant (P<0.05) genotypic differences were found among the individual genotypes. An additional phenotypic selection would, therefore, be advantageous after performing a marker-based selection.

References

  1. Anderson JA, Stack RW, Liu S, Waldron BL, Fjeld AD, Coyne C, Moreno-Sevilla B, Fetch JM, Song QJ, Cregan PB, Frohberg RC (2001) DNA markers for Fusarium head blight resistance QTL in two wheat populations. Theor Appl Genet 102:1164–1168CrossRefGoogle Scholar
  2. Bai G, Kolb FL, Shaner G, Dornier LL (1999) Amplified fragment length polymorphism markers linked to a major quantitative trait locus controlling scab resistance in wheat. Phytopathology 89:343–347CrossRefPubMedGoogle Scholar
  3. Buerstmayr H, Lemmens M, Hartl L, Doldi L, Steiner B, Stierschneider M, Ruckenbauer P (2002) Molecular mapping of QTL for Fusarium head blight resistance in spring wheat. I. Resistance to fungal spread (type II resistance). Theor Appl Genet 104:84–91PubMedCrossRefGoogle Scholar
  4. Buerstmayr H, Steiner B, Hartl L, Griesser M, Angerer N, Lengauer D, Miedaner T, Schneider B, Lemmens M (2003) Molecular mapping of QTL for Fusarium head blight resistance in spring wheat. II. Resistance to fungal penetration and spread. Theor Appl Genet 107:503–508PubMedCrossRefGoogle Scholar
  5. Dudley JW (1993) Molecular markers in plant improvement: manipulation of genes affecting quantitative traits. Crop Sci 33:660–668CrossRefGoogle Scholar
  6. Eshed Y, Zamir D (1996) Less-than-additive epistatic interactions of quantitative trait loci in tomato. Genetics 143:1807–1817PubMedGoogle Scholar
  7. Fehr WR (1987) Principles of cultivar development, vol 1. Theory and technique. Macmillan Publishing Company, New YorkGoogle Scholar
  8. Littell RC, Milliken GA, Stroup WW, Wolfinger RD (1996) SAS system for mixed models. SAS Institute Inc., Cary, publications order no. 55235Google Scholar
  9. Mesterházy Á, Bartok T, Mirocha CG, Komoroczy R (1999) Nature of wheat resistance to Fusarium head blight and the role of deoxynivalenol for breeding. Plant Breed 118:97–110CrossRefGoogle Scholar
  10. Miedaner T, Gang G, Geiger HH (1996) Quantitative-genetic basis of aggressiveness of 42 isolates of Fusarium culmorum for winter rye head blight. Plant Dis 80:500–504CrossRefGoogle Scholar
  11. Miedaner T, Reinbrecht C, Lauber U, Schollenberger M, Geiger HH (2001) Effects of genotype and genotype × environment interaction on deoxynivalenol accumulation and resistance to Fusarium head blight in rye, triticale, and wheat. Plant Breed 120:97–105CrossRefGoogle Scholar
  12. Miedaner T, Moldovan M, Ittu M (2003a). Comparison of spray and point inoculation to assess resistance to Fusarium head blight in a multienvironment wheat trial. Phytopathology 93:1068–1072CrossRefPubMedGoogle Scholar
  13. Miedaner T, Schneider B, Geiger HH (2003b) Deoxynivalenol (DON) content and Fusarium head blight resistance in segregating populations of winter rye and winter wheat. Crop Sci 43:519–526CrossRefGoogle Scholar
  14. Miedaner T, Heinrich N, Schneider B, Oettler G, Rohde S, Rabenstein F (2004) Estimation of deoxynivalenol (DON) content by symptom rating and exoantigen content for resistance selection in wheat and triticale. Euphytica 139:123–132CrossRefGoogle Scholar
  15. Mueller H-M, Reimann J, Schumacher U, Schwadorf K (1997) Fusarium toxins in wheat harvested during six years in an area of Southwest Germany. Nat Toxins 5:24–30PubMedGoogle Scholar
  16. Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier M-H, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023PubMedGoogle Scholar
  17. Saghai Maroof MAK, Soliman RA, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer length polymorphism in barley: mendelian inheritance, chromosomal location and population dynamics. Proc Natl Acad Sci USA 81:8014–8018PubMedCrossRefGoogle Scholar
  18. Sanchez AC, Brar DS, Huang N, Li Z, Khush GS (2000). Sequence tagged site marker-assisted selection for three bacterial blight resistance genes in rice. Crop Sci 40:792–797CrossRefGoogle Scholar
  19. SAS Institute Inc. (2001) SAS/STAT user’s guide, version 6, 4th edn. Cary, NCGoogle Scholar
  20. Schroeder HW, Christensen JJ (1963) Factors affecting resistance of wheat to scab caused by Gibberella zeae. Phytopathology 53:831–838Google Scholar
  21. Shen X, Zhou M, Lu W, Ohm H (2003) Detection of Fusarium head blight resistance QTL in a wheat population using bulked segregant analysis. Theor Appl Genet 106:1041–1047PubMedGoogle Scholar
  22. Smith KP, Evans CK, Dill-Macky R, Gustus C, Xie W, Dong Y (2004) Host genetic effect on deoxynivalenol accumulation in Fusarium head blight of barley. Phytopathology 94:766–771CrossRefPubMedGoogle Scholar
  23. Snijders CHA (1990) The inheritance of resistance to head blight caused by Fusarium culmorum in winter wheat. Euphytica 50:11–18CrossRefGoogle Scholar
  24. Somers DJ, Fedak G, Savard M (2003) Molecular mapping of novel genes controlling Fusarium head blight resistance and deoxynivalenol in spring wheat. Genome 46:555–564PubMedCrossRefGoogle Scholar
  25. Steiner B, Lemmens M, Griesser M, Scholz U, Schondelmaier J, Buerstmayr H (2004) Molecular mapping of resistance to Fusarium head blight in the spring wheat cultivar Frontana. Theor Appl Genet 109:215–224PubMedCrossRefGoogle Scholar
  26. Utz HF, Melchinger AE, Schön CC (2000) Bias and sampling error of the estimated proportion of genotypic variance explained by quantitative trait loci determined from experimental data in maize using cross validation and validation with independent samples. Genetics 154:1839–1849PubMedGoogle Scholar
  27. Waldron BL, Moreno-Sevilla B, Anderson JA, Stack RW, Frohberg RC (1999) RFLP mapping of QTL for Fusarium head blight resistance in wheat. Crop Sci 39:805–811CrossRefGoogle Scholar
  28. Zhou WC, Kolb FL, Bai GH, Shaner G, Domier LL (2002) Genetic analysis of scab resistance QTL in wheat with microsatellite and AFLP markers. Genome 45:719–727PubMedCrossRefGoogle Scholar
  29. Zhou WC, Kolb FL, Bai GH, Domier LL, Boze LK, Smith NJ (2003) Validation of a major QTL for scab resistance with SSR markers and use of marker-assisted selection in wheat. Plant Breed 122:40–46CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • T. Miedaner
    • 1
  • F. Wilde
    • 1
  • B. Steiner
    • 2
  • H. Buerstmayr
    • 2
  • V. Korzun
    • 3
  • E. Ebmeyer
    • 3
  1. 1.State Plant Breeding InstituteUniversity of Hohenheim (720)StuttgartGermany
  2. 2.Department for Agrobiotechnology Tulln, Institute of Plant Production BiotechnologyBOKU-University of Natural Resources and Applied Life Sciences ViennaTullnAustria
  3. 3.Lochow-Petkus GmbHBergenGermany

Personalised recommendations