Theoretical and Applied Genetics

, Volume 112, Issue 3, pp 455–461 | Cite as

Targeting xa13, a recessive gene for bacterial blight resistance in rice

  • Zhaohui Chu
  • Binying Fu
  • Hong Yang
  • Caiguo Xu
  • Zhikang Li
  • A. Sanchez
  • Y. J. Park
  • J. L. Bennetzen
  • Qifa Zhang
  • Shiping Wang
Original Paper

Abstract

Bacterial blight, caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most serious diseases of rice worldwide. Thirty bacterial blight resistance (R) genes (21 dominant genes and 9 recessive genes) in rice have been identified. They are the main sources for the genetic improvement of rice for resistance to Xoo. However, little is known about the recessive R genes. To clone and characterize the recessive R genes, we fine-mapped xa13, a fully recessive gene for Xoo resistance, to a DNA fragment of 14.8 kb using the map-based cloning strategy and a series of sequence-based molecular markers. Sequence analysis of this fragment indicated that this region contains only two apparently intact candidate genes (an extensin-like gene and a homologue of nodulin MtN3) and the 5′ end of a predicted hypothetical gene. These results will greatly facilitate the isolation and characterization of xa13. Four PCR-based markers, E6a, SR6, ST9 and SR11 that were tightly linked to the xa13 locus, were also developed. These markers will be useful tools for the marker-assisted selection of xa13 in breeding programs.

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Zhaohui Chu
    • 1
  • Binying Fu
    • 2
    • 4
  • Hong Yang
    • 1
  • Caiguo Xu
    • 1
  • Zhikang Li
    • 2
    • 4
  • A. Sanchez
    • 2
  • Y. J. Park
    • 3
  • J. L. Bennetzen
    • 3
  • Qifa Zhang
    • 1
  • Shiping Wang
    • 1
  1. 1.National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan)Huazhong Agricultural UniversityWuhanChina
  2. 2.Plant Breeding, Genetics and Biotechnology DivisionInternational Rice Research InstituteManilaThe Philippines
  3. 3.Department of GeneticsUniversity of GeorgiaAthensUSA
  4. 4.Chinese Academy of Agricultural SciencesInstitute of Crop SciencesBeijingChina

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