Extension and use of a physical map of the Thinopyrum-derived Lr19 translocation
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Twenty-nine deletion mutant lines were used to extend a physical map of the Lr19 translocated chromosome segment. One hundred and forty-four Sse8387I/MseI and 32 EcoRI/MseI primer combinations were used to obtain 95 Thinopyrum-specific AFLP markers. The physical map confirmed that terminal deletions had mostly occurred, however, it appears that intercalary deletions and primer or restriction site mutations were also induced. The markers allowed for grouping of the deletion mutant lines into 19 clusters, with 7 AFLP markers mapping in the same marker bin as Lr19. Primary and secondary Lr19 allosyndetic recombinants were subsequently physically mapped employing AFLP, RFLP, SCAR and microsatellite markers and the data integrated with the deletion map. A further shortened, tertiary Lr19 recombinant was derived following homologous recombination between the proximally shortest secondary recombinant, Lr19-149-299, and distally shortest recombinant, Lr19-149-478. The tertiary recombinant could be confirmed employing the mapped markers and it was possible to identify new markers on this recombinant that can be used to reduce the translocation still further.
Key wordsDeletion mapping Gamma irradiation Homoeologous pairing Allosyndetic pairing
The University of Stellenbosch, Winter Cereal Trust and National Research Foundation provided facilities and funding. AFLP primer sequences and RFLP probe PSR129 were kindly supplied by the John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom. RFLP probe TtksuE018 was obtained from the Wheat Genetics Resource Centre, Kansas State University, Manhattan, KS 66506-5502, USA.
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