Molecular mapping of a quantitative trait locus for aluminum tolerance in wheat cultivar Atlas 66
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Genetic improvement of aluminum (Al) tolerance is one of the cost-effective solutions to improve wheat (Triticum aestivum) productivity in acidic soils. The objectives of the present study were to identify quantitative trait loci (QTL) for Al-tolerance and associated PCR-based markers for marker-assisted breeding utilizing cultivar Atlas 66. A population of recombinant inbred lines (RILs) from the cross Atlas 66/Century was screened for Al-tolerance by measuring root-growth rate during Al treatment in hydroponics and root response to hematoxylin stain of Al treatment. After 797 pairs of SSR primers were screened for polymorphisms between the parents, 131 pairs were selected for bulk segregant analysis (BSA). A QTL analysis based on SSR markers revealed one QTL on the distal region of chromosome arm 4DL where a malate transporter gene was mapped. This major QTL accounted for nearly 50% of the phenotypic variation for Al-tolerance. The SSR markers Xgdm125 and Xwmc331 were the flanking markers for the QTL and have the potential to be used for high-throughput, marker-assisted selection in wheat-breeding programs.
KeywordsTriticum aestivum Aluminum tolerance SSR marker QTL mapping
This paper reports the results of research only. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. This is contribution No. 05-78-J of the Kansas Agricultural Experiment Station, Manhattan, KS, USA.
- Basu U, McDonald JL, Archamhault DJ, Good AG, Briggs KG, Aung T, Taylor GJ (1997) Genetic and physiological analysis of doubled-haploid, aluminum-resistant lines of wheat provide evidence for the involvement of a 23 kDa, root exudate polypeptide in mediating resistance. Plant Soil 196:283–288CrossRefGoogle Scholar
- Gupta PK, Balyan HS, Edwards, KJ, Isaac P, Korzun V, Roder M, Gautier MF, Joudrier P, Schlatter AR, Dubcovsky J, Pena RC, Khairallah M, Penner G, Hayden MJ, Sharp P, Keller B, Wang RCC, Hardouin JP, Jack P, Leroy P (2002) Genetic mapping of 66 new microsatellite (SSR) loci in bread wheat. Theor Appl Genet 105:413–422CrossRefPubMedGoogle Scholar
- Guyomarc’h H, Sourdille P, Edwards KJ, Bernard M (2002) Studies of the transferability of microsatellite derived from Triticum taushchii to hexaploid wheat and to diploid related species using amplification, hybridization and sequence comparisons. Theor Appl Genet 105:736–744CrossRefPubMedGoogle Scholar
- Hoekenga OA, Vision TJ, Shaff JE, Monforte AJ, Lee GP, Howell SH, Kochian LV (2003) Identification and charcterization of aluminum tolerance loci in Arabidopsis (Landsberg erecta × Columbia) by quantitative trait locus mapping. A physiologically simple but genetically complex trait. Plant Physiol 132:936–948CrossRefPubMedPubMedCentralGoogle Scholar
- Jackson T, Reisenauer H (1984) Crop response to lime in western United States. In: Adams F (ed) Soil acidity and liming. American Society of Agronomy, Crop Science Society of America, Soil society of America, Madison, WI, pp 333–347Google Scholar
- Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulk segregant analysis. A rapid method to detect markers in specific renomic regions by using sepregating population. Proc Natl Acad Sci USA 88:9828–9832CrossRefPubMedPubMedCentralGoogle Scholar
- Prestes AM, Konzak CF, Hendrix JW (1975) An improved seedling culture method for screening wheat for tolerance to toxic levels of aluminum. In: Agronomy abstracts. ASA, Madison, WI, p 60Google Scholar
- SAS institute Inc. (1989) SAS/STAT user’s guide, Version 6, 4th edn. Cary, NCGoogle Scholar
- Wood S, Seastian K, Scherr S (2000) Soil resource condition. In: Pilot analysis of global ecosystems. International Food Policy Research Institute and The World Resources Institute, Washington, DC, pp 45–54Google Scholar